O-Antigen Description

Serogroup:O56
Serotype H:
Serotype K:
Strain:
Structure:
->7)aNeu5Ac(2->3)bDGlc(1->3)bDGlcNAc(1->
                    |
           aDGal(1->2)
Comment:- Revision [1,2] of previous structure [4]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfaP

bDGlc(1->

->3)bDGlcNAc

1 | 1
GT NameDonorAcceptorScore / e-value
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

218 / 2e-71
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

191 / 9e-61
O165-WcnW


185 / 9e-59
O82-O082_09


182 / 7e-58
O30-O030_06


150 / 1e-45
O143-WfdV


147 / 7e-44
O140-O140_14


140 / 2e-41
O93-O093_02


134 / 3e-39
O91-WbsA


134 / 4e-39
O161-weiG

bDGlcA(1->

->3)bDGlcNAc

133 / 6e-39
O112-WfeI


131 / 3e-38
O102-WelO

bDGlc(1->

->3)bDGalNAc

131 / 3e-38
O182-O182_10


128 / 5e-37
O38-O038_09


123 / 3e-35
O21-WclQ


120 / 4e-34
O24-WfaM

bDGlc(1->

->3)bDGalNAc

118 / 5e-33
O130-WffJ


115 / 5e-32
O134-O134_08


113 / 1e-31
O46-O046_08


112 / 5e-31
O27-WejU


112 / 3e-31
O157-WbdN


111 / 1e-30
O61-WeiL


108 / 6e-30
O79-WbdX


107 / 4e-29
O81-O081_11


105 / 3e-28
O128-WbsK

bDGalNAc(1->

->4)aDGal

84 / 7e-21
O113-WbnA


82 / 3e-20
O46-O046_05


82 / 3e-20
O134-O134_05


82 / 3e-20
O55-WbgP


81 / 8e-20
O55-WbgP


81 / 8e-20
O12-O012_O2


80 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfaQ

aDGal(1->

GT NameDonorAcceptorScore / e-value
O24-WfaO

aDGlc(1->

->2)bDGlc

275 / 5e-91
O152-WfgE


275 / 2e-91
O173-WcmX

aDGlc(1->

->2)bDGlc

263 / 2e-86
O29-WffY


188 / 9e-58
O130-WffI


160 / 6e-47
O81-O081_10


159 / 5e-47
O18-wcxD


158 / 1e-46
O144-O144_03


150 / 7e-44
O169-O169_04


134 / 2e-37
O66-WfaB


128 / 2e-35
O183-wbaT


127 / 2e-35
O85-WfbQ


125 / 2e-34
O102-WelN

aDGal(1->

->6)bDGlc

123 / 8e-34
O27-WejX


109 / 9e-29
O110-O110_03


104 / 4e-27
O187-O187_10


103 / 4e-27
O178-O178_05


90 / 2e-22
O153-O153_02


90 / 2e-22
O101-O101_06


86 / 1e-20
O34-O034_11


86 / 9e-21
O87-WfgO


83 / 1e-19
O89-O089_06


83 / 8e-20
O65-O065_10


82 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfaR

aNeu5Ac(2->

->3)bDGlc

3 | 2/3
GT NameDonorAcceptorScore / e-value
O24-WfaN

aNeu5Ac(2->

->3)bDGlc

582 / 0.0
O104-WbwA

aNeu5Ac(2->

->3)bDGal

90 / 2e-22
NMR
1H-NMR2.00 2.51 3.89 4.06 3.73 3.77 3.80 3.50 4.69 3.66 4.16 3.51 3.51 3.98 3.79 4.73 3.65 4.07 3.51 3.57 4.04 3.81 5.52 3.82 3.92 4.00 4.12 3.80 3.80
Solvent: not stated
Temp: NULL
pD:
Ref: Not Stated NULL ppm
Comment: 1H-NMR [4]
13C-NMR173.30 101.95 37.45 72.00 53.70 72.60 78.40 69.15 63.20 102.80 56.90 78.20 70.10 76.30 61.90 101.60 73.20 80.20 69.40 75.80 61.90 96.80 69.15 70.10 70.00 71.00 61.90
Solvent: not stated
Temp: NULL
pD:
Ref: Not Stated NULL ppm
Comment: 13C-NMR [4]
Enzymes
FlippaseMKLMKDRFFYFFIELINKGVPFFLLPYVSSAVGVEVYGKIELFTTNYIILSFLFALGFEGWMNSHYFKIS ENNFITILSSFYFLSFLIFFISVITTLLYSSDWTALVIVGYIQSLLNITTLHLRLKGKYIRSGALLLAAS IVNAVMVYVCFELFGKDYNARIEAFLFSSVLLVIFIILCNINIWGGGGKNKINFSFLNKDILLFCLPLCL TMVINWAKGNIDKYFIANLLDLKSLGIYAVAYQLASVINVVGIILNRTLQADLLKSMADGFYQTKKIILI FLSVLAIFFLLYVIAVCYGFNYVFSADYAPSRNMALLLIFCFLFFSTSSFLINFVLFYGRPKLILLQSLL ITTIHVALSFLLIQKYALFGALFTQVITSILTFISTLLLCYILMKASHK
Trembl: Q077Q8
PolymeraseMIPYIIFYSLSLTLVVLSLVLKKYDWFWLLFLLFISIIFIGLRVNVGADYTEYVRIYNQSYDTNFELGFD IIFNLFKRFGYDYVFVSLFVFILTSFFFLYSIKSLQHKTLIYFCFLLFMFVPLTSTIRQGLAIPFFIICL LNADRPKVYFMSIAIGCFFHYSILFMLFFFFVRYFKQSYCRAYFIILFFLGLSVFNIVEYMIILLHYIPS LGTLSSKLLMYTQRYNVTLSLTSVAYKLAGLLLVTSFMSFIKINKKLLCSYNLYYISLCLFILFKDNSVF TNRLMYATNISLVTFFSFLLSMMNAFYRKAIVTFLFVIYFSINYFKFTATDLRHSIESAYMPYKSVLIN
Trembl: Q077Q9
Literature
  • First Author: Torgov, VI. "NMR reinvestigation of two N-acetylneuraminic acid-containing O-specific polysaccharides (O56 and O24) of Escherichia coli". Carbohydrate Research. 272(1). (1995), pp 73-90 PubMed: [7544238]
  • First Author: Gamian, A. "Structure of the Escherichia coli O24 and O56 O-specific sialic-acid-containing polysaccharides and linkage of these structures to the core region in lipopolysaccharides". European Journal of Biochemistry. 225(3). (1994), pp 1211-1220 PubMed: [7525286]
  • First Author: Cheng, J. "Characterization of E. coli O24 and O56 O antigen gene clusters reveals a complex evolutionary history of the O24 gene cluster". Current Microbiology. 53(6). (2006), pp 470-476 PubMed: [17072668]
  • First Author: Kogan, G. "Structure of the O56 antigen of Escherichia coli, a polysaccharide containing 7-substituted alpha-N-acetylneuraminic acid". Carbohydrate Research. 238. (1993), pp 261-70 PubMed: [7679326]
  • First Author: Brockhausen, I. "Characterization of two beta-1,3-glucosyltransferases from Escherichia coli serotypes O56 and O152". Journal of Bacteriology. 190. (2008), pp 4922-32 PubMed: [18487334]