O-Antigen Description

Serogroup:O128 ab
Serotype H:
Serotype K:
Strain:
Structure:
  ->6)bDGal(1->3)bDGalNAc(1->4)aDGal(1->3)bDGalNAc(1->
         |
aLFuc(1->2)
Comment:- Structure [1]
- Signals from O-acetylation at 2.16ppm and 2.18ppm in a 2:1 ratio have also been reported in [3]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbsH

aDGal(1->

->3)bDGalNAc

1 | 1
GT NameDonorAcceptorScore / e-value
O55-WbgM


372 / 4e-130
O55-WbgM

aDGal(1->

->3)bDGalNAc

371 / 1e-129
O110-O110_02


336 / 1e-115
O113-WbnE


254 / 2e-83
O69-O069_12


244 / 9e-80
O3-WclR


243 / 4e-79
O18-wekW


228 / 2e-73
O40-WffO


215 / 2e-68
O19-O019_11


164 / 5e-49
O148-WbbG

aDGlc(1->

->3)aDGlcNAc

160 / 1e-47
O51-O051_12


152 / 2e-44
O87-WfgO


145 / 5e-42
O120-O120_15


138 / 3e-39
O22-WbhP


114 / 2e-30
O142-O142_12


103 / 3e-27
O76-O076_08


94 / 1e-23
O89-O089_06


88 / 9e-22
O142-O142_03


84 / 2e-20
O101-O101_06


83 / 8e-20
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

82 / 1e-19
O86-WbnH


81 / 3e-19
O86-WbwH


81 / 3e-19
O86-WcmA

aDGalNAc(1->

->3)bDGalNAc

81 / 4e-19
O127-WbiN


81 / 3e-19
O90-O090_04


81 / 3e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbsJ

aLFuc(1->

->2)bDGal

2 | 4
GT NameDonorAcceptorScore / e-value
O55-WbgN


157 / 4e-47
O55-WbgN

aCol(1->

->2)bDGal

157 / 4e-47
O86-WbnK

aLFuc(1->

->2)bDGal

145 / 1e-42
O86-WbwK

aLFuc(1->

->2)bDGal

145 / 2e-42
O86-WcmD

aLFuc(1->

->2)bDGal

145 / 1e-42
O126-WbgL


140 / 1e-40
O127-WbiQ


123 / 2e-34
O90-O090_14


123 / 2e-34
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbsK

bDGalNAc(1->

->4)aDGal

3 | 2
GT NameDonorAcceptorScore / e-value
O125-O125_04


150 / 3e-44
O55-WbgO


134 / 2e-38
O55-WbgO


134 / 2e-38
O113-WbnA


134 / 2e-38
O167-WfdH


133 / 6e-38
O86-WbnJ

bDGal(1->

->3)aDGalNAc

126 / 6e-36
O86-WcmC

bDGal(1->

->3)aDGalNAc

126 / 8e-36
O86-WbwJ


125 / 2e-35
O46-O046_08


120 / 1e-33
O134-O134_08


120 / 1e-33
O127-WbiP


117 / 1e-32
O90-O090_13


117 / 1e-32
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

113 / 7e-31
O23-O023_06


112 / 8e-31
O48-O048_06


110 / 1e-29
O102-WelM

bDGalNAc(1->

->3)aDGal

107 / 2e-28
O27-WejU


107 / 8e-29
O157-WbdN


105 / 5e-28
O74-O074_09


104 / 7e-28
O61-WeiL


102 / 2e-27
O165-WcnW


102 / 2e-27
O24-WfaM

bDGlc(1->

->3)bDGalNAc

99 / 1e-25
O54-O054_06


99 / 9e-26
O81-O081_11


99 / 8e-26
O130-WffJ


97 / 2e-25
O130-WffH'


97 / 8e-25
O91-WbsA


97 / 2e-25
O112-WfeI


96 / 7e-25
O21-WclQ


95 / 2e-24
O37-O037_11


95 / 2e-24
O82-O082_09


94 / 3e-24
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

94 / 3e-24
O102-WelO

bDGlc(1->

->3)bDGalNAc

94 / 3e-24
O30-O030_06


94 / 3e-24
O126-WbsT


93 / 8e-24
O71-WdaK

bDGal(1->

->3)aDGalNAc

93 / 1e-23
O93-O093_02


91 / 5e-23
O64-O064_03


90 / 1e-22
O143-WfdV


89 / 2e-22
O22-WbhQ


89 / 2e-22
O38-O038_09


88 / 3e-22
O117-WbeA


86 / 2e-21
O185-O185_03


86 / 4e-21
O107-WclW


85 / 4e-21
O63-WbuP


84 / 1e-20
O70-O070_09


84 / 1e-20
O92-O092_07


84 / 1e-20
O146-WbwW


81 / 1e-19
O182-O182_10


81 / 8e-20
O140-O140_14


81 / 6e-20
O43-O043_04


80 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbsL

bDGal(1->

->3)bDGalNAc

4 | 3
GT NameDonorAcceptorScore / e-value
O76-O076_03


132 / 2e-37
NMR
1H-NMR4.53 3.99 3.90 4.05 3.68 3.80 3.80 5.08 3.68 3.87 4.11 3.82 4.59 4.03 3.81 4.15 3.66 3.80 3.80 4.61 3.65 3.81 3.90 3.77 3.79 3.79 5.24 3.78 3.66 3.73 4.22 1.21 2.05 2.05
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [1]
13C-NMR104.70 52.80 78.20 69.60 75.90 62.40 96.90 69.60 70.50 78.00 71.90 61.50 102.60 51.90 77.20 65.40 76.10 62.40 103.30 77.30 74.90 70.00 74.20 69.30 100.40 69.50 70.90 73.10 68.00 16.50 23.60 23.80 175.25 175.35
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 31.45 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMNKKEIIILLIARILQILISLFTMRLMTTILSNEQMGEYYIFLAIYMLASLGIINPIGQY VNRNTYLWWREGVLTQYINRYINFVLCFSLIYVILYGLGECLFKGINTPFWMLFCYLYIV CLSLNQFLLHTLNILNFKIVFAITTLMTSVLSLSGAYLFIEYSIAKQTVSIYYWVAGIII GNFISLCICVVYLRSKNTFQKSILKSINVSIKEVFNFCCPIMITTLFIWFQSSGYRFAVE QMFGLKFLGILAVCFAVSGQIMSVIESLVTQIFQPTLFKHMDNVKNRKQHLDFYINENIA IYASVLFFCTFFMREIFLVLVDNKYLPYFYIGIIALWSEFFRISTNTLSLVFFCEKKMKV GIFPYLIGSLSLAFSIVLFRLLNVNLGILIAFAIGVAQLASFIVCLITVKKISYFKINSI FLMKRIIIVLPSVLIAHCIPKFEMINAIHFIIIFGVGLFYLISLATSFMFIKQPGGVYGS
Trembl: Q6XQ54
PolymeraseMISYGVLFLFSYINAVLQKRVTVTLLFFIMIIVSFFKYNIGFDWGNYHEIYLQQGDGFFF YEPLFSLLIILSNKVALEYKYFISMCSLITLIVYYSGMRCFARDIKELALALTYFVVSYC YFEHFNIIRQTLAIALFFYSWKYIIETNFIMYSLFIFFASMIHAAAVVLLPFYFFLRIDN FKPVYYGIVVLFIIIVVRFDINSILLSSLNLFKNFDFVARYLANSTDLADFASKKSDLGL SKMYPAIIFVFCCFWGGIRLESRRDYVFFNAGFIAITLLLLSYQVDILFRFFSFFETSFV LLYCIIFSGFNKKSKIILHMVNLIVYTVMTFNMLSVMGLIPYESYIF
Trembl: Q6XQ50
Literature
  • First Author: Sengupta, P. "Structure of the O-specific side chain of the Escherichia coli O128 lipopolysaccharide". Carbohydrate Research. 277. (1995), pp 283-290 PubMed: [8556736]
  • First Author: Shao, J. "Sequence of Escherichia coli O128 antigen biosynthesis cluster and functional identification of an alpha-1,2-fucosyltransferase". FEBS Letters . 553. (2003), pp 99-103 PubMed: [14550554]
  • First Author: Ali T. "Structural studies of the O-antigenic polysaccharides from the enteroaggregative Escherichia coli strain 87/D2 and international type strains from E. coli O128". Carbohydrate Research. 343. (2008), pp 695-702 PubMed: [18237721]