O-Antigen Description

Serogroup:O173
Serotype H:H-
Serotype K:K-
Strain:CCUG 36541
Structure:
aLFuc(1->4)
         |
  ->3)aDGlc(1->P-6)aDGlc(1->2)bDGlc(1->3)bDGlcNAc(1->
Comment:- Structure [1].
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcmU

aLFuc(->

->4)aDGlc

1 | 4
GT NameDonorAcceptorScore / e-value
O41-O041_06


123 / 3e-35
O43-O043_13


122 / 2e-34
O11-WdaG


119 / 1e-33
O125-O125_13


119 / 9e-34
O43-O043_04


119 / 9e-34
O11-WdaD


117 / 4e-33
O36-O036_08


117 / 1e-32
O168-WfeY


108 / 1e-29
O184-O184_15


108 / 2e-29
O156-O156_14


108 / 1e-29
O41-O041_12


107 / 5e-29
O84-O084_13


107 / 2e-29
O36-O036_15


105 / 2e-28
O159-WfgS


103 / 6e-28
O168-WfeV


98 / 1e-25
O157-WbdO


90 / 5e-23
O111-WbdL

aDGlc(1->

->4)aDGal

86 / 2e-21
O159-WfgP


81 / 8e-20
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcmV

aDGlc(1->

P->6)aDGlc

2 | 3
GT NameDonorAcceptorScore / e-value
O152-WfgC


344 / 3e-118
O160-O160_07


196 / 5e-61
O181-O181_03


145 / 6e-42
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcmW

bDGlc(1->

->3)bDGlcNAc

3 | 1
GT NameDonorAcceptorScore / e-value
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

235 / 4e-78
O165-WcnW


209 / 5e-68
O82-O082_09


192 / 1e-61
O56-WfaP

bDGlc(1->

->3)bDGlcNAc

176 / 3e-55
O38-O038_09


147 / 3e-44
O112-WfeI


145 / 3e-43
O61-WeiL


139 / 4e-41
O93-O093_02


138 / 1e-40
O130-WffJ


138 / 1e-40
O143-WfdV


138 / 2e-40
O140-O140_14


137 / 3e-40
O46-O046_08


136 / 5e-40
O182-O182_10


135 / 1e-39
O134-O134_08


131 / 5e-38
O102-WelO

bDGlc(1->

->3)bDGalNAc

126 / 3e-36
O21-WclQ


124 / 3e-35
O30-O030_06


123 / 6e-35
O161-weiG

bDGlcA(1->

->3)bDGlcNAc

122 / 9e-35
O27-WejU


122 / 7e-35
O81-O081_11


118 / 7e-33
O79-WbdX


115 / 3e-32
O91-WbsA


115 / 3e-32
O128-WbsK

bDGalNAc(1->

->4)aDGal

113 / 6e-31
O157-WbdN


111 / 1e-30
O24-WfaM

bDGlc(1->

->3)bDGalNAc

108 / 1e-29
O55-WbgP


102 / 3e-27
O55-WbgP


101 / 4e-27
O113-WbnA


92 / 2e-23
O102-WelM

bDGalNAc(1->

->3)aDGal

87 / 6e-22
O12-O012_O2


87 / 1e-21
O48-O048_06


86 / 2e-21
O46-O046_05


86 / 3e-21
O134-O134_05


86 / 3e-21
O51-O051_11


86 / 2e-21
O111-WbdL

aDGlc(1->

->4)aDGal

84 / 7e-21
O55-WbgO


83 / 1e-20
O32-WfeA


83 / 3e-20
O130-WffH'


83 / 2e-20
O55-WbgO


83 / 1e-20
O117-WbeA


83 / 2e-20
O125-O125_04


83 / 3e-20
O107-WclW


81 / 6e-20
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcmX

aDGlc(1->

->2)bDGlc

4 | 2
GT NameDonorAcceptorScore / e-value
O152-WfgE


325 / 1e-110
O24-WfaO

aDGlc(1->

->2)bDGlc

324 / 2e-110
O56-WfaQ


263 / 2e-86
O29-WffY


177 / 1e-53
O81-O081_10


160 / 2e-47
O18-wcxD


151 / 4e-44
O85-WfbQ


146 / 8e-42
O130-WffI


137 / 2e-38
O144-O144_03


131 / 1e-36
O102-WelN

aDGal(1->

->6)bDGlc

123 / 8e-34
O187-O187_10


121 / 6e-33
O27-WejX


120 / 2e-32
O169-O169_04


117 / 9e-32
O183-wbaT


115 / 6e-31
O66-WfaB


103 / 9e-27
O34-O034_11


102 / 2e-26
O110-O110_03


92 / 5e-23
O65-O065_10


89 / 6e-22
O101-O101_06


82 / 2e-19
O178-O178_05


81 / 4e-19
O153-O153_02


81 / 4e-19
O89-O089_06


80 / 9e-19
NMR
1H-NMR5.44 3.63 4.03 3.68 3.91 3.86 3.86 5.24 3.52 3.73 4.06 3.51 4.06 4.12 4.97 3.76 3.86 3.77 4.84 1.25 4.93 3.78 3.99 3.39 3.42 3.61 3.93 4.68 3.42 3.53 3.43 3.40 3.70 3.89 2.06
Solvent: D2O
Temp: 50C
pD: 8
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1], pD=8
13C-NMR96.10 73.40 77.90 72.80 73.20 60.40 98.80 72.60 73.50 71.70 69.80 65.10 99.20 68.80 70.20 72.90 67.40 16.30 101.80 56.40 80.00 70.10 76.10 62.60 102.00 78.80 75.70 70.60 76.70 61.40
Solvent: D2O
Temp: 50C
pD: 8
Ref: Acetone 31.00 ppm
Comment: 13C-NMR [1], pD=8
Non-Sugar Components
- Phosphate
Enzymes
FlippaseMNKKVLLNIFSLLSIQGVGYILPLITLPYLVRILGPNNYGVYGYSLAIIQYSILIIDFGFNLTVTKKISL NRDDKLIVSSVFWNVMACKLLLCIFTFLITVAWSRIDNTFAGDNLAVLMSSYTMVIGILIFPIWLFQGKE EITKIALINISSKFLTVPLIFILIDKPSDGWKVGLINGSGYIVAGIIGLHIVSRRNWIVWVKPTFSQMFR EYASAWHIFISNASINIYTSSITVILGALTSPVTVGYFVAADKIRLAFQGLINPVSQALYPRINALMKES PAAGFKTIRLLLRTQGVFFFFCSGFIFYLSPNIISFLYGESYKESIPILKILAWLPFIIAISNVLGYQTL LVLNMERIFSRLVLVGGGIALVIIFPLIIYFGGYGAALTILIAEVSVNL
Trembl: E2DNQ8
PolymeraseMLETYFIYNFIMLCGIFFAFVKENTHSIIFKVASSLMLIFVIWIPSGIRYGIGTDYFSYLRIFQSALEGR VTTEIGYYLINYLVVVTGGEGYYIFLISSLIIALVIVINMPRHYSCLYVLIFFCIIYLPSFSLVRQSLAV VFLLVSIKHAIENKILLSYFFALVAISFHLSSLLILPFIILRKVRVPPLLALCMIVFCIIFFLSTNILQD IINSSFIASTKYGFYADTKFGGEAIIGTGGGVLIKALLPLMIILQSSKIVKSQPNCNIIILLSIVAICAS IASLRIHIFSRVSDLFMFLPLLGVTYLMNSFSKKITKQICACLIFLSMIVLFERTIATNLSENNSGLGVS PYTTIFDVR
Trembl: E2DNR6
Literature
  • First Author: Linnerborg, M. "Structural studies of the O-antigen polysaccharide from the enteroinvasive Escherichia coli O173". Carbohydrate Research. 320. (1999), pp 200-208 PubMed: [10573858]
  • First Author: Quan Wang. "Development of a serogroup-specific multiplex PCR assay to detect a set of Escherichia coli serogroups based on the identification of their O-antigen gene clusters". Molecular and Cellular Probes. 24. (2010), pp 286-290 PubMed: [20561581]