O-Antigen Description

Serogroup:O42
Serotype H:37
Serotype K:-
Strain:P11a
Structure:
->2)DGro(1-P-4)bDGlcNAc(1->3)bDGalf2Ac(1->3)aDGlcNAc(1->
                     |   
            aDGlc(1->3)
Comment:- Structure [2]
- Structure was determined for strain CCUG 11348
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbeS

(R)Gro(1-P->

->4)bDGlcNAc

1 | 3
GT NameDonorAcceptorScore / e-value
O28-WbeS

DGro(1-P->

->4)bDGlcNAc

653 / 0.0
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbeX

bDGlcNAc(1->

->3)bDGalf

2 | 2
GT NameDonorAcceptorScore / e-value
O28-WbeX

bDGlcNAc(1->

->3)bDGalf

659 / 0.0
O96-O096_07


107 / 2e-28
O112-WfeF


100 / 1e-25
O160-O160_05


88 / 1e-21
O160-O160_04


88 / 1e-21
O117-WbeA


84 / 1e-20
O115-WegI


84 / 2e-20
O107-WclW


82 / 7e-20
O171-O171_11


82 / 1e-19
O185-O185_09


81 / 4e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbeY

bDGalf(1->

->3)aDGlcNAc

3 | 1
GT NameDonorAcceptorScore / e-value
O28-WbeY

bDGalf(1->

->3)aDGlcNAc

626 / 0.0
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

251 / 5e-83
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

234 / 2e-76
O124-WfeT

bDGalf(1->

->3)bDGalNAc

219 / 2e-70
O164-WfeT


219 / 2e-70
O96-O096_08


213 / 3e-68
O170-O170_08


211 / 2e-67
O170-O170_O4


150 / 4e-44
NMR
1H-NMR5.32 3.49 3.69 3.46 3.64 3.81 5.22 4.86 4.35 4.27 3.93 3.66 3.68 2.15 5.02 4.07 3.87 3.48 3.87 3.82 3.86 2.06 4.74 3.81 3.97 4.21 3.55 3.81 3.90 2.08 4.03 4.07 3.92 3.77
Solvent: D2O
Temp: 42C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [2]
13C-NMR100.01 72.52 73.35 69.88 73.13 60.97 106.81 82.47 83.40 83.30 71.11 63.86 21.06 173.41 97.93 53.98 77.98 69.10 73.15 61.46 22.97 174.82 100.99 55.45 79.70 74.87 75.80 61.33 23.52 175.09 65.55 78.17 62.11
Solvent: D2O
Temp: 42C
pD:
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [2]
Non-Sugar Components
- Acetate - Glycerol - Phosphate
Enzymes
FlippaseMSLILTDFGFNLSSTEYIARNCQSKDKINKHIGAVFQIKILLIFLCVIFLYFLVNDQLQQFKIEFFFYSC IAIIGQTFQSLWFFQGVEKMKRITLFLTISKFSYVILTFVIVKKPEQGRFVILAYALSQVIGALLSQYYM RKEGYKYTPANLSSMLGVLRTSALFFWSRLAVSTYTTASTLVVGAAGHVQAGYYAASEQLYKAGQNITTP VSQALYPYMTRAKNWALFFKMMSILTVVMITASLTAIWLSQWLIALIFGPEYIVSQNVFIVFMLALIVTF VSTNFGYPVFSALGKVKVANYTVIFGALTHVVILGVLFCLGEISAYNVVRAVLFTEIVILILRMFFFVKL YRREVK
Trembl: A4K7Z5
PolymeraseMKQARIKPFSYLSLSFFVLFLIFLNISAVSFDEYLTDLQYSYAYVLFAISLVAVYYAQGESYTSLGFLFN TCLFLFIGGRFIATTFGIDSESVFTLDFMTTYIPTSDEKLEIYTRVVSFQIFLNLGYLFLRKEDSLLNLN KNDNSLPGRLLFLIVIVLFLLCLVEQYSMLSKAMSEGYLARYGDQAESYDAGAAFKYTLLFVFTGLLFAT CKKKYIIINFMIYMTLATIIILIGARGAFVSTILMGVWLYGLNKKISLKKLSIIFLSLAGILLIIMNFSA RGNYYSNELIDSLLRFLYDQGNTLAVVGYVRTITDYPVFAYISTFLPGSSFFASMFHGGSDLNSYMLSFP AYANYVANPNMYFAGYGLGWSIISSVEMFSADILFIFCIFSVLIGMFLKFANEQAKRNSFWFGAVACLCA KILFTARADIKTIIPLFMYYAIMYLIISTAMRILRQRYG
Trembl: A4K7Z7
Literature
  • First Author: Fratamico PM. "Sequencing of the Escherichia coli O2, O28ac, O63 and O42 O antigen clusters and detection of pathogenic strains by multiplex PCR assays targeting virulence genes in the respective O-antigen gene clus". Not listed.
  • First Author: Fontana C. "Structural studies and biosynthetic aspects of the O-antigen polysaccharide from Escherichia coli O42". Carbohydrate Research. 403. (2015), pp 174-181 PubMed: [24909379]