O-Antigen Description

Serogroup:O43
Serotype H:
Serotype K:
Strain:Bi7455-41
Structure:
aLFuc(1->3)
         |
  ->4)bDMan2Ac(1->3)aDMan(1->3)aLFuc(1->3)bDGalNAc(1->
Comment:- Structure [2]
- The first mannose residue is O-acetylated 70%
- Strain used for structure elucidation was G1247
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
O043_04

bDGalNAc(1->

GT NameDonorAcceptorScore / e-value
O11-WdaD


174 / 8e-55
O43-O043_13


124 / 1e-35
O184-O184_15


123 / 3e-35
O125-O125_13


122 / 1e-34
O11-WdaG


121 / 2e-34
O173-WcmU

aLFuc(->

->4)aDGlc

119 / 9e-34
O84-O084_13


115 / 2e-32
O159-WfgS


114 / 5e-32
O36-O036_15


113 / 2e-31
O168-WfeY


112 / 3e-31
O156-O156_14


110 / 2e-30
O41-O041_12


108 / 1e-29
O168-WfeV


92 / 8e-24
O36-O036_08


88 / 2e-22
O157-WbdO


87 / 5e-22
O111-WbdL

aDGlc(1->

->4)aDGal

83 / 1e-20
O12-O012_O2


82 / 5e-20
O41-O041_06


81 / 5e-20
O128-WbsK

bDGalNAc(1->

->4)aDGal

80 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
O043_06
GT nameDonorAcceptorPosition | Link.NumSimilar to
O043_08

GT NameDonorAcceptorScore / e-value
O184-O184_10


320 / 3e-109
O11-WdaF


218 / 2e-69
O36-O036_10


201 / 4e-63
O125-O125_08


194 / 1e-60
O99-WejJ

aDRha(1->

->3)aDRha

94 / 7e-24
O175-O175_05


86 / 4e-21
O141-WfcC


86 / 1e-20
O64-O064_19


81 / 4e-19
O187-O187_12


80 / 7e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
O043_13

GT NameDonorAcceptorScore / e-value
O125-O125_13


496 / 0.0
O11-WdaG


488 / 0.0
O184-O184_15


385 / 4e-137
O41-O041_12


261 / 3e-88
O159-WfgS


260 / 6e-88
O84-O084_13


259 / 7e-88
O168-WfeY


258 / 3e-87
O36-O036_15


257 / 7e-87
O156-O156_14


257 / 8e-87
O11-WdaD


135 / 8e-40
O43-O043_04


124 / 1e-35
O173-WcmU

aLFuc(->

->4)aDGlc

122 / 2e-34
O36-O036_08


112 / 9e-31
O111-WbdL

aDGlc(1->

->4)aDGal

109 / 1e-29
O168-WfeV


108 / 2e-29
O41-O041_06


95 / 1e-24
O157-WbdO


88 / 2e-22
NMR
1H-NMR5.05 3.82 4.09 3.87 4.77 1.27 4.85 4.26 3.83 4.02 3.46 3.75 3.93 5.12 4.23 4.14 3.80 3.80 3.79 3.89 5.00 3.83 4.06 3.96 4.14 1.20 4.49 4.04 3.76 3.95 3.62 3.74 3.74 2.03
Solvent: D2O
Temp: 40C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [2]
13C-NMR96.0 69.4 70.8 73.3 68.0 16.5 98.3 68.5 75.7 71.9 77.0 61.5 103.2 68.5 78.7 66.5 74.4 62.3 102.6 68.8 78.2 72.7 68.3 16.4 102.0 52.9 79.6 69.0 76.1 62.6 176.2 23.4
Solvent: D2O
Temp: 40C
pD:
Ref: TSP -1.6 ppm
Comment: 13C-NMR [2]
Literature
  • First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22(1). (2015), pp 101-7 PubMed: [25428893]
  • First Author: Perepelov AV. "Structure and gene cluster of the O-antigen of Escherichia coli O43". Carbohydrate Research. 416. (2015), pp 32-36 PubMed: [26342864]