O-Antigen Description

Serogroup:O111
Serotype H:
Serotype K:
Strain:
Structure:
aCol(1->6)
        |
 ->4)aDGlc(1->4)aDGal(1->3)bDGlcNAc(1->
        |
aCol(1->3)
Comment:- Structure [1]. Very poor NMR data - caution
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdH

aDGal(1->

->3)bDGlcNAc

1 | 1
GT NameDonorAcceptorScore / e-value
O101-O101_07


258 / 1e-85
O89-O089_07


257 / 6e-85
O103-WbtG


208 / 3e-66
O100-O100_11


148 / 2e-43
O121-WbqI


137 / 3e-39
O10-O010_12


136 / 9e-39
O138-WfaH

aDGalNAcA(1->

->3)bDGlcNAc

131 / 9e-37
O133-O133_08

aDGalA(1->

->3)aDGlcNAc

125 / 6e-35
O158-wcmT


123 / 7e-34
O116-O116_11


114 / 6e-31
O52-Orf16


104 / 3e-27
O113-WbnE


82 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdL

aDGlc(1->

->4)aDGal

2 | 2
GT NameDonorAcceptorScore / e-value
O36-O036_08


112 / 2e-30
O184-O184_15


110 / 6e-30
O43-O043_13


109 / 1e-29
O11-WdaG


106 / 2e-28
O125-O125_13


106 / 2e-28
O156-O156_14


101 / 1e-26
O168-WfeY


100 / 3e-26
O84-O084_13


98 / 2e-25
O159-WfgS


97 / 4e-25
O41-O041_12


94 / 4e-24
O36-O036_15


92 / 1e-23
O11-WdaD


91 / 4e-23
O173-WcmU

aLFuc(->

->4)aDGlc

86 / 2e-21
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

84 / 8e-21
O43-O043_04


83 / 2e-20
O12-O012_O2


82 / 6e-20
O41-O041_06


81 / 1e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbdM

aCol(1->

->3)aDGlc
->6)aDGlc

3 | 3, 4
GT NameDonorAcceptorScore / e-value
O18-wekV

aDGlc(1->

->4)aDGal

270 / 1e-89
O142-O142_12


264 / 1e-87
O116-O116_10


257 / 7e-85
O103-WbtF


253 / 2e-83
O101-O101_06


181 / 1e-55
O89-O089_06


179 / 7e-55
O120-O120_15


112 / 4e-30
O87-WfgO


110 / 3e-29
O121-WbqH


101 / 2e-26
O113-WbnE


95 / 3e-24
O55-WbgM


86 / 4e-21
O55-WbgM

aDGal(1->

->3)bDGalNAc

86 / 3e-21
O3-WclR


84 / 4e-20
O18-wekW


83 / 5e-20
O128-WbsH

aDGal(1->

->3)bDGalNAc

82 / 1e-19
O22-WbhT


81 / 2e-19
O22-WbhP


81 / 2e-19
NMR
13C-NMR102.00 101.00 82.00 79.00 78.00 77.00 75.00 74.50 73.00 72.00 70.50 69.00 68.00 65.00 63.00 61.50 56.00 35.00 34.50 24.00 17.40 17.10
Solvent: not stated
Temp: NULL
pD:
Ref: Not Stated NULL ppm
Comment: 13C-NMR [4]. Manually extracted from picture. Shifts +/-=0.5 ppm! Some signals very broad, some arise from several overlapping resonance. A signal at 31.0 ppm is possibly acetone.
Enzymes
FlippaseMVLTVKKILAFGYSKVLPPVIEQFVNPICIFIITPLILNHLGKQSYGNWILLITIVSFSQ LICGGCSAWIAKIIAEQRILSDLSKKNALRQISYNFSIVIIAFAVLISFLILSICFFDVA RNNSSFLFAIIICGFFQEVDNLFSGALKGFEKFNVSCFFEVITRVLWASIVIYGIYGNAL LYFTCLAFTIKGMLKYILVCLNITGCFINPNFNRVGIVNLLNESKWMFLQLTGGVSLSLF DRLVIPLILSVSKLASYVPCLQLAQLMFTLSASANQILLPMFARMKASNTFPSNCFFKIL LVSLISVLPCLALFFFGRDILSIWINPTFATENYKLMQILAISYILLSMMTSFHFLLLGI GKSKLVANLNLVAGLALAASTLIAAHYGLYAISMVKIIYPAFQFYYLYVAFVYFNRAKNV
Trembl: Q46722
PolymeraseMSIDLLFSITEIAIVFSCTIYIFTQCLLMRRIYLDKSILILLCLLFFLVIIQLPELNVNG LVDSLKLSLPLLMVFIAFQKPKLCLWVIIALLFLNSAFNFLYLKTFDKFSSFPFTFFILL FYLFRLGIGNLPVYKNKKFYALIFLFILIDIMQSLLINYRGQILYSVICILILVFKVNLR KKIPYFFLMLPVLYVIIMAYIGFNYFNKGVTFFEPTASNIERTGMIYYLVSQLGDYIFHG MGTLNFLNNGGQYKTLYGLPSLIPNDPHDFLLRFFISIGVIGALVYHSIFFVFFRRISFL LYERNAPFIVVSCLLLLQVVLIYTLNPFDAFNRLICGLTVGVVYGFAKIR
Trembl: Q9S521
Literature
  • First Author: Eklund, K. "Structural studies on the Escherichia coli O111 lipopolysaccharide. Abstr 493, In Abstracts of the IXth International Symposium of Carbohydrate Chemistry, London". Not specified. (1978),
  • First Author: Kenne, L. "Structural studies of the O-antigens from Salmonella greenside and Salmonella adelaide". Carbohydrate Research. 111(2). (1983), pp 289-96 PubMed: [6339053]
  • First Author: Edstrom, RD. "The biosynthesis of cell wall lipopolysaccharide in Escherichia coli. VII. Studies on the structure of the O-antigenic polysaccharide". Journal of Biological Chemistry. 242(18. (1967), pp 4125-4133 PubMed: [4863044]
  • First Author: Gupta, R. "Comparative immunogenicity of conjugates composed of Escherichia coli O111 O-specific polysaccharide, prepared by treatment with acetic acid or hydrazine, bound to tetanus toxoid by two synthetic sche". Infection and Immunity. 63(8). (1995), pp 2805-2810 PubMed: [7542631]
  • First Author: Wang, L. "Sequencing of Escherichia coli O111 O-antigen gene cluster and identification of O111-specific genes". Journal of Clinical Microbiology. 36(11). (1998), pp 3182-3187 PubMed: [9774562]
  • First Author: Stevenson, G. "Determination of glycosyltransferase specificities for the Escherichia coli O111 O antigen by a generic approach". Applied and Environmental Microbiology. 74(4). (2008), pp 1294-1298 PubMed: [18156323]