O-Antigen Description

Serogroup:O170
Serotype H:
Serotype K:
Strain:CCUG36538
Structure:
   ->4)bDGlcNAc(1->4)bDGlcA(1->3)bDGalf(1->3)bDGlcNAc(1->
          |
bDGalf(1->3)
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
O170_O4

bDGalf(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O170-O170_08


180 / 2e-55
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

174 / 4e-53
O124-WfeT

bDGalf(1->

->3)bDGalNAc

165 / 8e-50
O164-WfeT


165 / 8e-50
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

163 / 5e-49
O96-O096_08


159 / 1e-47
O42-WbeY

bDGalf(1->

->3)aDGlcNAc

150 / 4e-44
O28-WbeY

bDGalf(1->

->3)aDGlcNAc

149 / 7e-44
O167-WfdJ


104 / 2e-27
O96-O096_04


87 / 1e-21
GT nameDonorAcceptorPosition | Link.NumSimilar to
O170_05

GT NameDonorAcceptorScore / e-value
O91-WbsG


131 / 8e-38
O182-O182_09


127 / 3e-36
O38-O038_03


82 / 4e-20
GT nameDonorAcceptorPosition | Link.NumSimilar to
O170_07

GT NameDonorAcceptorScore / e-value
O171-O171_12


115 / 1e-31
O185-O185_07


83 / 3e-20
GT nameDonorAcceptorPosition | Link.NumSimilar to
O170_08

bDGalf(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

309 / 1e-105
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

308 / 2e-105
O124-WfeT

bDGalf(1->

->3)bDGalNAc

287 / 5e-97
O164-WfeT


287 / 5e-97
O96-O096_08


278 / 2e-93
O42-WbeY

bDGalf(1->

->3)aDGlcNAc

211 / 2e-67
O28-WbeY

bDGalf(1->

->3)aDGlcNAc

210 / 5e-67
O170-O170_O4


180 / 2e-55
O167-WfdJ


105 / 1e-27
O96-O096_04


90 / 1e-22
NMR
1H-NMR4.58 3.82 3.69 3.45 3.42 3.74 3.90 5.05 4.18 4.20 4.18 3.87 3.60 3.63 4.60 3.36 3.61 3.75 3.83 4.58 3.82 3.81 3.82 3.51 3.72 3.90 5.16 4.01 4.01 4.21 3.81 3.67 3.74 2.02 2.03
Solvent: 1
Temp: 30C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [2]
13C-NMR101.2 56.4 82.1 69.2 77.1 62.1 109.6 81.2 85.8 83.4 71.5 64.2 103.5 73.8 75.0 81.2 76.6 174.8 101.7 56.6 77.4 75.2 76.7 61.3 108.9 82.4 78.7 85.1 72.3 64.3 23.4 23.7 175.5 175.6
Solvent: D2O
Temp: 30C
pD:
Ref: TSP -1.6 ppm
Comment: 13C-NMR [2]
Enzymes
FlippaseMKLNTTLNKNILYLAVVQGSSYILPLITFPYLVRVLGPELFGVLGFCQASMQYLVLLTDY GFNWTATQQVAKNKKDIEKLTRIFWSVFFAKIFLASLAFIFLAACCFLIEKYKELWVVLF SFSPLVLGNVIYPVWFFQGMEKMKWITICTITARCLVIPLTFIFVKNGQDVWVAALIQGA VNLLAGLMGLCLIRKKRWVDKIIVDYNDIKQCLKDGWHVFISTSAISLYTTSTTVILGFV SGPIAVGYFNVANTIRNAAQGLLNPITQAIYPRINAMYDSDYPQAILLIKKTLRYVGALA LLGSIFLYMLAPTIINIGVGYDYEQAISILRWMSFLPFIIVLSNIFGVQTMLVHNFKKEF SRILVISGVTNLIIIYPLIRLFSGDGAAISLLITELMVSTLMYLFLRSKKIHLIY
Trembl: A0A0A8J624
PolymeraseMRNILNSTIGIFIVFTIFFPLSPFVFSSGNAGALTTICSIIIIFLLLQQKINVTNNLYML LFPVLLSFIISALYWQEIMLLNYPKYLIFSIIIVSLLQKNIVYIIGKYSTFVIVFALCCA VIGFIYAAIGGSAIYTITNPNGREFYFYLTTFTVTNYSGFIRPSAFFDEPGALSFYICFV VMLREILWPRKRALNFALLAFGIVTLSLAHIVFTILYIIYCLITKPTTKLIVGLVIILIL SFVFLAVLPNKDVVFEYLISRSSSDTDGQGRSALLQAAIDTLQNAGVGLYIFGIDSNCLV NYDEACRYIYPRMGENILSPVVFGGLLTSWLFYFSYIYVGYKSLRKFIPNFIIFSFLLLY LQRPYIYLLSYSFMFMIALRLSIGFFDKEK
Trembl: A0A0A8J7A3
Literature
  • First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
  • First Author: Shashkov AS. "Structure elucidation and biosynthesis gene cluster organization of the O-antigen of Escherichia coli O170". Carbohydrate Research. 417. (2015), pp 11-14 PubMed: [26382081]