O-Antigen Description

Serogroup:O179
Serotype H:
Serotype K:
Strain:E4378
Structure:
->4)aDGlcA(1->3)bDMan(1->4)bDMan(1->3)bDGlcNAc(1->
                   |
          bDGlc(1->2)
Comment:- Strain is given for the gene sequence study in reference [2]
- Structure presented at the 29th International Carbohydrate Symposium, Lisbon, Poster no BS2. 2018.
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
O179_03
GT nameDonorAcceptorPosition | Link.NumSimilar to
O179_06
GT nameDonorAcceptorPosition | Link.NumSimilar to
O179_07

bDMan(1->

->4)bDMan

GT NameDonorAcceptorScore / e-value
O6-Orf5

bDMan(1->

->4)bDMan

162 / 2e-48
GT nameDonorAcceptorPosition | Link.NumSimilar to
O179_08

bDMan(1->

GT NameDonorAcceptorScore / e-value
O106-WbaD


381 / 4e-133
O73-WbaD

bDMan(1->

->3)aDGlcNAc

381 / 4e-133
O77-WbaD

bDMan(1->

->3)aDGlcNAc

381 / 4e-133
O44-O044_03


369 / 1e-128
O17-O017_03


369 / 1e-128
O6-Orf7


350 / 2e-121
O62-O062_12


330 / 2e-113
O59-WclC


216 / 6e-69
O176-O176_05


87 / 1e-21
O119-WekK


87 / 2e-21
NMR
1H-NMR5.42 3.63 3.89 3.72 4.09 4.83 4.44 3.90 3.86 3.48 3.79 3.94 4.71 3.92 3.81 3.81 3.54 3.76 4.64 3.35 3.52 3.52 3.36 3.80 3.93 4.63 3.86 3.74 3.59 3.52 3.80 3.94
Solvent: D2O
Temp: 48C
pD:
Ref: TSP 0.0 ppm
Comment:
13C-NMR99.8 72.1 71.3 81.6 73.2 175.5 101.2 78.6 76.2 67.6 76.8 61.5 101.3 70.9 72.1 77.5 75.7 61.5 104.4 74.3 76.1 69.7 76.5 60.7 100.90 55.00 84.6 69.0 76.1 60.7
Solvent: D2O
Temp: 48C
pD:
Ref: Dioxane 67.4 ppm
Comment:
Enzymes
FlippaseMNFLVYLALSIVSKLIASVREFGVGFIWGTGSIVDAFILAMMLPMFFIDIASVMFNSTYIGIYVNSANKE RRFFFKDVFYFTLIYVLFTNIIVIFFLKEYIAYSDIPIETKHIIDNIIIYIVVYYSLLAFCEFYKAHLMA CEKQKMMPLPMICANIVFISTMYYFSEGANDIALVSCFVFSSVIQFILYIPLIIKKDSVYIMNSSVSHSR EANKKLFLKNLVPVFFNAAVNQSSKITDKVIASSLIIGSLSSLYFAQQLYALYVNIIIVSVLSFAYPKIC KKANENKYEFSSYIVQVIIVLFVLLVYPVMLGWRYAQDIFKFILVNSTTQNIKVVSEMFMVLLSVVVFES ISASLKRAFWALNKMKVTVYISGFTVIINIILSVLLSSYYGPKGLVYSYCAATILSTMLLIFYFNKVSKI TIPKNIFLPIMTASIISVTFYILLGFFRIDLNKPISLLIPGLIVVLGMFLLFVCFNKNSISKVLKE
Trembl: A0A0A8J7J6
PolymeraseMFTISPVFFENITFQGSRTLADVLFCISLLLIICKHKKVNISLLGIVYIIIVLVIMSVNA ALGFSNASTTINNELFDKLTWLILDRNINEFIPFGDYYRNILIYTRYLLIPFYFWMGLYY SKLVGKERAIVTLYNGLIICLLLNMLYGIYNDDDRLTGFFENTAALSSLALLCMLLSLFT KSKYKIILSWVIGIIALLMSQTASAFIGLTAVVLILVFNIRRHHKKTVYITSLLSVVIAS IGLMPEIMEFIARYMYTGSLINRINTWSTIINYFDSYQVAILGLGSFPLFADNIFIWLIS GFGIFSFVIYMYLVSIGNKSQKSALFISLLLWQGVLFPGFIMPYMLMTTFITLGILSNNK GCENECI
Trembl: A0A0A8J9D5
Literature
  • First Author: Scheutz F. "Designation of O174 and O175 to temporary O groups OX3 and OX7, and six new E. coli O groups that include Verocytotoxin-producing E. coli (VTEC): O176, O177, O178, O179, O180 and O181". Acta Pathologica, Microbiologica, et Immunologica Scandinavica. 112. (2004), pp 569-584 PubMed: [15601305]
  • First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
  • First Author: Fontana C. "Structural Studies and Biosynthetic Aspects of the O-Antigen Polysaccharide from Shiga-toxin-Producing E. coli O179". Carbohydrate Research. inprep. (2018), pp Poster