O-Antigen Description

Serogroup:O59
Serotype H:19
Serotype K:
Strain:
Structure:
aDGalA(1->3)
          |
   ->6)aDMan(1->3)aDMan(1->3)bDMan(1->3)aDGlcNAc(1->
Comment:- Structure [2]
- aDGalA is O-acetylated in position 3 and 4 with a 3:7 ratio in the native LPS
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclA
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclB

aDMan(1->

->3)aDMan

GT NameDonorAcceptorScore / e-value
O99-WejJ

aDRha(1->

->3)aDRha

129 / 1e-35
O60-O060_11


123 / 6e-34
O64-O064_19


122 / 1e-33
O9-MtfB

aDMan(1->

->3)aDMan

120 / 8e-33
O141-WfcC


110 / 2e-29
O88-O088_12


102 / 1e-26
O7-WbbC

aDMan(1->

->4)bDGal

90 / 3e-22
O9-MtfA

aDMan(1->

->2)aDMan

88 / 1e-21
O169-O169_06


83 / 6e-20
O36-O036_10


82 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclC

bDMan(1->

->3)aDGlcNAc

GT NameDonorAcceptorScore / e-value
O106-WbaD


241 / 4e-78
O73-WbaD

bDMan(1->

->3)aDGlcNAc

241 / 4e-78
O77-WbaD

bDMan(1->

->3)aDGlcNAc

241 / 4e-78
O62-O062_12


229 / 5e-74
O179-O179_08


216 / 7e-69
O6-Orf7


214 / 3e-68
O44-O044_03


209 / 3e-66
O17-O017_03


209 / 3e-66
O119-WekK


84 / 5e-20
NMR
1H-NMR5.26 3.87 3.99 4.33 4.46 5.13 4.24 4.01 4.15 4.00 3.61 4.15 5.10 4.16 3.98 3.84 3.83 3.85 4.89 4.13 3.94 3.63 3.77 3.84 2.05 4.74 3.99 3.72 3.72 3.44 3.76 3.93
Solvent: D2O
Temp: 60C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [2]
NMR data is given for the LPS-OH
13C-NMR101.73 69.46 70.56 71.58 73.06 176.19 103.47 70.62 80.72 65.69 72.53 65.74 103.19 70.66 79.85 66.59 74.16 61.60 97.86 53.27 82.33 69.38 72.53 61.45 22.90 174.97 101.18 71.44 81.61 66.78 76.94 61.76
Solvent: D2O
Temp: 60C
pD:
Ref: Dioxane 67.40 ppm
Comment: 13-NMR [2]
NMR data is given for the LPS-OH
Enzymes
FlippaseMSNYIKNAGWIFFEKFVRIAISFMLFAFVSRVLNPNDFGVLSYYQTITTMLLALTNLGFD NVLINEFNQNEKHNKIFATAFWSRIIVSTVVILLFAVGVCFYDLPMVNKIVLLLCLSSLV FQTQNTYVSYFQSQLQASIITKISFLSLLLSALVKVYLLMTDKNIIWFAFSYSFDFAVSF LFFAVFSYKRNYVSISLRYFDLNVLKGLLRSSWPIIASAVIVVLYTRLDQLMIMNMLGSD SVAIFSVAIKISEAYLFVPAALVTSYYPLISKSPSNDNIRFYFDVVFATSIIMAVGVAII SFFLIPIMFGEYYACSYTILIVLLLGSLFSILGSACTNLMIVYGLSYLRLIRAIFGLLVN FSLNLIFIPKYGVIGAAYASVVSQIFAAWVSNAFSKKTIECFKLQTITVLTCGVLGGRQL CQILFKK
Trembl: Q4KZ30
PolymeraseMKKSEFQAEKLIILFYFIINLCTFIIITSSSIYIGDPAGEYYTVPLDIAIILFISNTAIY YLLYLIYRCCVSHIAFHNEVIIKRIETLLFLGVTIALLGFFWIDYGRAEYQSTSPFGFVF RLIPYSVIWLLYVSINSKWNAKSIFLIVYYVAAKIIMGWSGILLALFWVLFIKYYSAKKR SFLFMCMCMTLLVILYFSAPIVYYLKYYIRYAGQFEFNYPVLLSKLTARLSTIQNVLYIY QNTNVFTNLYNKYLFDGFYFIEPITSILPRALLGISATNFETIYVNIVTGEFNPGVIFYL GLFGKLLAYFNTGIYNFGNFFIVTFVLLGGLFILLKLNFKKAANPFIFYTIIQFALSGSI EELSYTLYGICLVLIFCKIRFGIHNENRY
Trembl: Q4KZ36
Literature
  • First Author: Guo, H. "Characterization of the Escherichia coli O59 and O155 O-antigen gene clusters: the atypical wzx genes are evolutionary related". FEMS Microbiology. 248(2). (2005), pp 153-61. PubMed: [15990253]
  • First Author: Fontana C. "Rapid structural elucidation of polysaccharides employing predicted functions of glycosyltransferases and NMR data: Application to the O-antigen of Escherichia coli O59". Glycobiology. 24(5). (2014), pp 450-457 PubMed: [24558268]