O-Antigen Description

Serogroup:O77
Serotype H:
Serotype K:96
Strain:
Structure:
->6)aDMan(1->2)aDMan(1->2)bDMan(1->3)aDGlcNAc(1->
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbaC

aDMan(1->

->2)DMan

1 | 2, 3
GT NameDonorAcceptorScore / e-value
O73-WbaC

aDMan(1->

->2)bDMan

674 / 0.0
O106-WbaC


672 / 0.0
O44-O044_02


641 / 0.0
O17-O017_02


641 / 0.0
O176-O176_06


192 / 1e-59
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbaD

bDMan(1->

->3)aDGlcNAc

2 | 1
GT NameDonorAcceptorScore / e-value
O106-WbaD


704 / 0.0
O73-WbaD

bDMan(1->

->3)aDGlcNAc

704 / 0.0
O44-O044_03


649 / 0.0
O17-O017_03


649 / 0.0
O179-O179_08


381 / 4e-133
O62-O062_12


372 / 2e-129
O6-Orf7


350 / 7e-121
O59-WclC


241 / 4e-78
O176-O176_05


86 / 8e-21
NMR
1H-NMR5.32 4.08 4.03 3.77 3.99 3.84 3.84 5.05 4.12 3.86 3.97 3.82 3.55 4.11 4.88 4.08 3.95 3.56 3.76 3.80 3.88 4.76 3.97 3.72 3.65 3.42 3.76 3.94 2.06
Solvent: D2O
Temp: 45C
pD:
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1]
13C-NMR99.90 78.80 70.40 67.00 73.00 61.20 103.00 70.40 71.20 66.40 72.00 65.40 97.60 53.50 80.60 68.80 72.30 61.10 100.40 76.30 74.30 67.30 77.30 61.40 22.60 174.20
Solvent: D2O
Temp: 45C
pD:
Ref: Dioxane 67.4 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMIKEIFSDGIFSLFNKICYFGFKTLAIILITRSLGEDVGGEFIFLVGFLEVLRVVTDFGVDLFVIKKYND STDKIKLLNVTFAQKILSGVIVLLLFCLYSFVQGYQYNVYIPIVLALPVALFFNFSNSYFQSLNSNKSLT FPIAIASIATCTIFAIQYFLNVKFNAWDYLCVELLFVSLVTLKLFVSVKFRFIRTSAFDVLMSILSLYKY TLSIGLTAVVVIIYSRIDNFYLKTFAPGDLAAYGQIFRMVDPLVMVSSVFSTVAYARFCRINLQEKNAFT KVFQFLLCIVAYGVLSSLVYFFIIKYLGHFVLISNVNNDFLIMMFLIVACVKCINGGTTAIIQSQGFYKV GLYVSYVCILTSIPTMYFLIMKYGVKGAAMTILLVECISFILLLCSFFMIKKMRPKKTFEM
Trembl: Q1L817
PolymeraseMHKQYYSLLCNKIQFKHLAIYFSVFLSLSSCFLWGGADTFVSQVYIDILATISTALLVVLMFYYYINRVR YLSLTLLFAFVFSLIIGLPSIYLYYFKNANHGFEIICIWGMLINSILYLQTTETVIKEEKRYLTSLFYIL FSIVALCQLYKILIYLMFILNSGEGHLAIYTESEELLSQVPFFIRAISGFAVVMSLSTFYFKTPTYIKVI AFVLLASDLAIGIRNKFFFSVICIFILYMYSNEYRVRNLFKQLSKPIFLIAGFLCFSMVSYFREGYQIDF MIYIGIVLDTLSSTLGGMQNLFDMQSSGWDQLHPAVVFTQVLPLSGLGFLDDQQIAKDFSIIVLGDVSSG IALSSSGLLESSLLSLNLGVIIYLLYLSGVMLFIKWGLNNQRIIINFVAISMISGFFYAVRGELILPLAF LLKSLPIVLISPLLVKNKIYHGQ
Trembl: Q1L818
Literature
  • First Author: Wang, W. "A group of Escherichia coli and Salmonella enterica O antigens sharing a common backbone structure". Microbiology. 153. (2007), pp 2159-2167 PubMed: [17600060]
  • First Author: Yildirim, H. "Structural studies of the O-polysaccharide from the Escherichia coli O77 lipopolysaccharide". Carbohydrate Research. 333. (2001), pp 179-183 PubMed: [11448680]
  • First Author: Zhou D. "Mass spectrometric characterization of a two-glycosyltransferase tandem reaction for assembly of tetrasaccharide repeating unit of Escherichia coli O77 O-antigen". Carbohydrate Research. (2016),