O-Antigen Description

Serogroup:	O91
Serotype H:
Serotype K:
Strain:
Structure:	->4)aDQui3NBuR3OH(1->4)bDGal(1->4)bDGlcNAc(1->4)bDGlcA6NGly(1->3)bDGlcNAc(1->
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsA

->3)bDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O161-weiG	bDGlcA(1->	->3)bDGlcNAc	287 / 2e-98
O182-O182_10			280 / 1e-95
O30-O030_06			277 / 1e-94
O93-O093_02			273 / 8e-93
O79-WbdX			235 / 3e-78
O143-WfdV			191 / 8e-61
O140-O140_14			183 / 4e-58
O38-O038_09			177 / 7e-56
O112-WfeI			152 / 3e-46
O165-WcnW			145 / 2e-43
O24-WfaM	bDGlc(1->	->3)bDGalNAc	134 / 3e-39
O152-WfgD	bDGlc(1->	->3)bDGlcNAc	132 / 2e-38
O102-WelO	bDGlc(1->	->3)bDGalNAc	132 / 2e-38
O82-O082_09			131 / 3e-38
O157-WbdN			125 / 7e-36
O46-O046_08			125 / 8e-36
O134-O134_08			124 / 2e-35
O130-WffJ			123 / 3e-35
O56-WfaP	bDGlc(1->	->3)bDGlcNAc	123 / 4e-35
O81-O081_11			123 / 6e-35
O173-WcmW	bDGlc(1->	->3)bDGlcNAc	115 / 3e-32
O61-WeiL			114 / 5e-32
O21-WclQ			111 / 1e-30
O27-WejU			110 / 3e-30
O128-WbsK	bDGalNAc(1->	->4)aDGal	97 / 2e-25
O4-WbuG	aLRha(1->	->6)aDGlc	81 / 7e-20

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsE

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsF

GT Name	Donor	Acceptor	Score / e-value
O49-O049_12			98 / 2e-25

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsG

GT Name	Donor	Acceptor	Score / e-value
O170-O170_05			131 / 8e-38
O182-O182_09			120 / 9e-34

ncbi CAZy

- Reference [2]

NMR
1H-NMR		4.52 3.67 3.75 4.04 3.78 3.88 3.81 4.54 3.42 3.66 3.90 3.97 3.84 4.58 3.74 3.81 3.47 3.46 3.77 3.98 2.03 4.58 3.74 3.72 3.72 3.63 3.82 4.02 2.03 4.87 3.67 4.08 3.44 4.27 1.23 2.46 2.53 4.24 1.26
Solvent:	D2O
Temp:	65C
pD:	9.0
Ref:	TSP 0.00 ppm
Comment:	1H-NMR [1], pD=9.0
13C-NMR		104.10 71.70 73.10 77.40 76.30 61.10 103.70 73.30 74.60 79.40 75.30 169.90 176.80 44.50 101.60 56.10 83.00 69.80 76.40 62.20 175.30 23.10 101.10 56.10 73.20 79.90 75.90 75.70 61.10 175.30 23.20 99.80 71.30 53.30 80.50 68.80 17.10 46.30 65.80 22.60
Solvent:	D2O
Temp:	65C
pD:
Ref:	Acetone 31.00 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- Glycine - (R)-3-hydroxybutyryl

Enzymes
Flippase		MRMLKTGLLNFISVLVKVITGLFLNKIMALYVGPAGYALVSQFQNGINFITTFASGAINT GVTKYTAEYANDKGKTSDLFKCITLLAITCCSFFSLTIIVFSEYFSLKLLGTINYSGVIK WFAATLFLFVANSILVSIINGLKEFKLYVLINIFGSLFSLLFSGILIYFFNLKGALIAFA TNQSIVFFMTFYLIISKKMVSLKSFIGMPSDFYFIKKLIQYALMAIVSAFCVIYMQIIIR TYIANHFGWVQAGIWDGVWRISSLYLMLVTVPLGVYYLPRLAELHTKSLLLREIKQGYLF LLPIIIISAIIIYYLRYFIIELLFSKSFVSMGDIIGWQLLGDIFKIGSWLLSYIMLSKAL TKTYIITEITTALLFLGGVHYLSPLYGLKGVTLAYCITYFCYWIMMIVVIISYFKGNFRN E
Trembl:	Q8GMJ7
Polymerase		MKASSVLTPYLNWFYGFLCFLSLWPFFSWKWPAIPVSFIAICFFLFLFDLCYINKLKVNK KIFLIIFSTLFVLILFILLPGGIPPWFNYYSLFLFLFLLLPRKRIFEISLKFRSIFIFSL LPGLVIYALLIIGVKLPYGILDAHNELKDSLGIYYRDYIGTVALSHLVLTVGDSTIIRFS GMLDEPGLLGTISALLLLADKLNFKHKSNYVLLLSGVISISLAFYLLILMGLIFQTRRKI ISLAIVFLIGIAIYHSPFYENFLQKRISDSESGLVFRDNRISDCFKEYYTAFENDSELNL FFGIGNNAHLRTGCDVSSYKMYIYDYGYLGFITIALLQIMQYMIPIMGGYACKFLKASFV FICLFILSYYQRPIFFNLAFSMIFYYSIFTYLKTVGISATNKK
Trembl:	Q8GMJ3

Literature
First Author: Kjellberg, A. "Structural determination and biosynthetic studies of the O-antigenic polysaccharide from the enterohemorrhagic Escherichia coli O91 using 13C-enrichment and NMR spectroscopy". Biochemistry. 38(38). (1999), pp 12205-11 PubMed: [10493787]
First Author: Perelle, S. "Identification of the O-antigen biosynthesis genes of Escherichia coli O91 and development of a O91 PCR serotyping test". Journal of Applied Microbiology. 93(5). (2002), pp 758-764 PubMed: [12392520]