O-Antigen Description

Serogroup:	O21
Serotype H:
Serotype K:	20
Strain:
Structure:	bDGal(1->4) \| ->3)bDGal(1->4)bDGlc(1->3)bDGalNAc(1-> \| bDGlcNAc(1->2)
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WclN

GT Name	Donor	Acceptor	Score / e-value
O81-O081_07			181 / 9e-57
O83-O083_05			145 / 2e-43
O32-WfeB			137 / 2e-40

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WclO

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WclP

GT Name	Donor	Acceptor	Score / e-value
O33-O033_07			107 / 2e-29

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WclQ

bDGlc(1->

->3)bDGalNAc

GT Name	Donor	Acceptor	Score / e-value
O130-WffJ			253 / 5e-85
O24-WfaM	bDGlc(1->	->3)bDGalNAc	239 / 1e-79
O134-O134_08			236 / 1e-78
O157-WbdN			234 / 1e-77
O61-WeiL			234 / 3e-78
O81-O081_11			234 / 7e-78
O46-O046_08			233 / 1e-77
O27-WejU			229 / 3e-76
O102-WelO	bDGlc(1->	->3)bDGalNAc	224 / 5e-74
O112-WfeI			185 / 9e-59
O38-O038_09			171 / 3e-53
O143-WfdV			167 / 2e-51
O140-O140_14			159 / 1e-48
O152-WfgD	bDGlc(1->	->3)bDGlcNAc	144 / 7e-43
O165-WcnW			142 / 2e-42
O173-WcmW	bDGlc(1->	->3)bDGlcNAc	124 / 3e-35
O182-O182_10			122 / 1e-34
O161-weiG	bDGlcA(1->	->3)bDGlcNAc	120 / 4e-34
O30-O030_06			119 / 2e-33
O82-O082_09			117 / 5e-33
O91-WbsA			111 / 9e-31
O56-WfaP	bDGlc(1->	->3)bDGlcNAc	109 / 5e-30
O93-O093_02			107 / 3e-29
O113-WbnA			97 / 2e-25
O128-WbsK	bDGalNAc(1->	->4)aDGal	95 / 1e-24
O79-WbdX			93 / 4e-24
O11-WdaD			82 / 4e-20

ncbi CAZy

- Reference [2]

NMR
1H-NMR		4.87 3.72 3.54 3.46 3.38 3.77 3.92 4.86 3.51 3.71 3.93 3.68 3.78 3.78 4.78 3.97 3.94 4.20 3.69 3.79 3.79 4.61 3.60 3.68 3.67 3.54 3.81 3.93 4.47 3.74 3.83 4.38 3.74 3.77 3.82 2.08 2.06
Solvent:	D2O
Temp:	75C
pD:	6.9
Ref:	TSP 0.00 ppm
Comment:	1H-NMR [1], pD=6.9
13C-NMR		102.20 56.80 75.10 70.90 76.80 61.80 103.60 72.40 73.90 69.70 75.80 61.90 104.00 52.50 80.60 68.70 75.50 62.00 103.00 79.90 76.50 79.50 75.30 60.90 103.90 71.30 82.50 76.10 75.30 61.60 23.80 23.10 175.40 175.20
Solvent:	D2O
Temp:	75C
pD:	6.9
Ref:	Dioxane 67.4 ppm
Comment:	13C-NMR [1], pD=6.9

Enzymes
Flippase		MFTIKTVFVLRIFGAILALSTSVMISRTLNMSEAGGVFFLLSVTGIISSLSSLGQNNLILKKCASVKYAL KARGVFFLRCIKRSVISSIILSIILIPVIHYFSPQLLLRNTWCLAILLIISSSVNILLYSFLQSQGLVTF SVVLQYIFQPLLFIFFVLLIVLIKNESVLLVSLSYFFSIILIGGGGGVYSYYKFRDDFNQLETESIPFRL KELSEYFIGNSLGMLIVQSYVVLSGLLLPAADVAIIAVSDRISLVINLFAMSISTILAPKVASLYSQSKM NEIKELTKKAMFFIMIPCVLMALLFPFFSGLILSIFGVQYSNASEVLIILVMTQIINAIFCPVYVFLNMS DRQGFISKLHIYMLIPSLVITFYLTNIFGVVGIAVSKLIVVSLINIIPLIYCILLSNRVIK
Trembl:	B5T081
Polymerase		MIYIIINTIVLFYMFLFNAYNRIPYGKLIIGGICVFFMTLLPAFQYGVGTDYFSYQNIYNNANELDTFYQ NKEYLFYGYMRLYQLTGWGFKGFIALTALLQSLLVFVIVFQLWKNYGYSLVLVFFLFWVVTNLFHTQMNI IRASFSIYLFAISILYKFRGKLLLSFILMVAALGFHRSALIGFCFLVIPDKMYFFACKHAFKIYVLTFLL FLFSYLQQIIYYIVQNLFPYYSHYLSSFDESSVSIMNVLTKMYWIPFNLLFLILLRLKVFVIKDNERKLI GMWALTVNVYLLMLSFDFISRVNYYFVIFYIIPIIYVIKYTVKNRYFVCLYLSLLYCFIPYLLKVILFPI AEFYYKSYLF
Trembl:	B5T085

Literature
First Author: Staaf, M. "Structural elucidation of the O-antigenic polysaccharides from Escherichia coli O21 and the enteroaggregative Escherichia coli strain 105". European Journal of Biochemistry. 266(1). (1999), pp 241-245 PubMed: [10542071]
First Author: Ren, Y. "Characterization of Escherichia coli O3 and O21 O antigen gene clusters and development of serogroup-specific PCR assays". Journal of Microbiological Methods. 75(2). (2008), pp 329-334 PubMed: [18700154]