O-Antigen Description

Serogroup:O3
Serotype H:2
Serotype K:2
Strain:
Structure:
bLRhaNAc(1->4)   aDGlc(1->4)
            |             |
     ->3)bDGlcNAc(1->3)aDGal(1->3)bDGlcNAc(1->
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclR

aDGal(1->

GT NameDonorAcceptorScore / e-value
O40-WffO


505 / 0.0
O113-WbnE


318 / 6e-108
O69-O069_12


282 / 2e-94
O18-wekW


256 / 1e-83
O128-WbsH

aDGal(1->

->3)bDGalNAc

243 / 4e-79
O110-O110_02


231 / 3e-74
O55-WbgM


208 / 1e-65
O55-WbgM

aDGal(1->

->3)bDGalNAc

206 / 3e-65
O148-WbbG

aDGlc(1->

->3)aDGlcNAc

142 / 2e-40
O19-O019_11


141 / 2e-40
O87-WfgO


140 / 9e-40
O51-O051_12


126 / 9e-35
O120-O120_15


124 / 4e-34
O121-WbqH


112 / 6e-30
O22-WbhP


95 / 5e-24
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

84 / 4e-20
O133-O133_08

aDGalA(1->

->3)aDGlcNAc

83 / 8e-20
O116-O116_10


81 / 3e-19
O76-O076_08


81 / 3e-19
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclS

GT NameDonorAcceptorScore / e-value
O109-weiN

bLRhaNAc3NAc(1->

->3)aDGlc

169 / 2e-50
O182-O182_07


151 / 4e-44
O119-WekJ

aLRhaNAc3Fo(1->

->3)bDMan

132 / 7e-37
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclU

bDGlcNAc(1->

->3)aDGal

GT NameDonorAcceptorScore / e-value
O142-O142_11


260 / 8e-88
O18-wekU

bDGlcNAc(1->

->3)aDGal

259 / 2e-87
O38-O038_06


135 / 3e-39
O23-O023_03


101 / 1e-26
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WclV
ncbi CAZy
- Reference [1]
NMR
1H-NMR5.42 3.92 3.81 4.26 4.16 5.02 4.60 3.73 3.24 3.42 1.32 4.98 3.49 3.69 3.46 3.72 4.62 3.73 3.78 3.93 3.37 4.59 3.76 4.11 3.64 3.45
Solvent: D2O
Temp: 70C
pD:
Ref: TSP 0.00 ppm
Comment: 1H-NMR [2]
13C-NMR99.80 72.50 79.00 76.50 72.30 99.90 53.90 73.00 73.50 73.30 17.50 99.60 72.80 73.70 70.50 72.00 100.50 55.70 79.90 68.90 76.50 104.00 56.30 78.00 74.80 74.80
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 31.00 ppm
Comment: 13C-NMR [2]
Enzymes
FlippaseMHVRVAVNFASKITFALISIITIPVIKSKLGVNAYGVLTFFLSIQVVILLLEGGMSATMARGLLNKKYKI YNISKAEYQSVYFIFFILVSLFVFSIFIVFNHNISTLIYNLDDREVYSFISADYVILLAGAMIALQFYII YYEALFVAYEKQVSYGIHIIVFNVIKTIISLFLLIVFNLGLDVYFLVHVFCTFILIILLHIKAIKNGLLE ITTLRVKKIYTKISFMKEEFHFSKNILCVSILSAIAFQADRLFITKYMGISAVGMYGIAYTLSTVPTLFT SSLYNVIYPRLIILTREQSSSAISFFELVSKLIYIIIIPTCVIISLNSTQILNIWIGKHEGIISLLLSIL IIATLFQGLQVIPFALQMAQERLKFTVFMNAIFVPSLILAYYLVSIHKILLYISIVWSVYNIITFFSLTI YLYAKNKMYTRLLIL
Trembl: B5T073
PolymeraseMIIFSTFLLFYFFIFLSLLPVLIKEGARGNGKYFYFFDLYIYLVLASLIIYIICTRDIFSDSDTERYLSI LEYYRINGFYLFDKNYIFAVYTYLYSLYIDGYYMYMIIPLITLLYSYCKLVKPLKYPVYSFSLVLIATFP VFWQLSASAFKQCIAISFILLALVHIIQTKYKQAVLLSLIASSFHFTALTFFIFSLFFYIRKERVSLNLF FLIWFFSVIFSISGLNSYIPSIIPFYDLVGESKVNIYLESTNNATGFRVDFFLFSLAPVLIIFINRTLVI KDRYIKMIALSYLYFNSIYNIMSFIPFSDRVAAYSWCLSPIILLWSALKCRSSIYMLSSIGIALLNPLIF MYYNSMFIFLK
Trembl: B5T075
Literature
  • First Author: Ren, Y. "Characterization of Escherichia coli O3 and O21 O antigen gene clusters and development of serogroup-specific PCR assays". Journal of Microbiological Methods. 75(2). (2008), pp 329-334 PubMed: [18700154]
  • First Author: Medina, EC. "Structural studies of the O-antigenic polysaccharides of Escherichia coli O3 and the enteroaggregative Escherichia coli strain 17-2". European Journal of Biochemistry. 224(1). (1994), pp 191-196 PubMed: [7521299]