O-Antigen Description

Serogroup:	O167
Serotype H:	5
Serotype K:
Strain:
Structure:	aDGalf(1->4) \| ->2)bDGalA6NLAla(1->3)aDGlcNAc(1->2)bDGalf(1->5)bDGalf(1->3)bDGlcNAc(1->
Comment:	- Structure [2]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfdF

GT Name	Donor	Acceptor	Score / e-value
O85-WfbR			100 / 7e-26

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfdG

bDGalA6NLAla(1->

->3)aDGlcNAc

2 |

GT Name	Donor	Acceptor	Score / e-value
O143-WfdR			155 / 3e-46
O46-O046_03			94 / 3e-24

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfdH

->3)aDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O128-WbsK	bDGalNAc(1->	->4)aDGal	133 / 6e-38
O86-WbnJ	bDGal(1->	->3)aDGalNAc	129 / 1e-36
O86-WcmC	bDGal(1->	->3)aDGalNAc	129 / 8e-37
O86-WbwJ			128 / 2e-36
O125-O125_04			120 / 3e-33
O127-WbiP			119 / 5e-33
O90-O090_13			119 / 5e-33
O23-O023_06			115 / 9e-32
O74-O074_09			110 / 8e-30
O113-WbnA			108 / 1e-28
O55-WbgO			107 / 1e-28
O55-WbgO			107 / 1e-28
O54-O054_06			105 / 3e-28
O71-WdaK	bDGal(1->	->3)aDGalNAc	93 / 9e-24
O174-WbcZ			92 / 1e-23
O143-WfdT			92 / 3e-23
O64-O064_03			91 / 6e-23
O65-O065_13			91 / 4e-23
O178-O178_09			86 / 4e-21
O153-O153_06			86 / 4e-21
O92-O092_07			86 / 2e-21
O7-WbbD	bDGal(1->	->3)aDGlcNAc	84 / 2e-20
O112-WfeJ			84 / 2e-20
O22-WbhQ			84 / 9e-21
O185-O185_03			84 / 1e-20
O24-WfaM	bDGlc(1->	->3)bDGalNAc	83 / 3e-20
O171-O171_13			83 / 3e-20
O37-O037_13			83 / 2e-20
O28-O028ab_02			83 / 3e-20
O107-WclZ	bDGal(1->	->3)aDGalNAc	82 / 9e-20
O114-WbuP	bDGal(1->	->3)aDGlcNAc	82 / 7e-20
O5-O05_15			82 / 7e-20
O117-WbeD	bDGal(1->	->3)aDGalNAc	82 / 8e-20
O160-O160_08			81 / 1e-19

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfdI

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfdJ

bDGalf(1->

->3)bDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O170-O170_08			105 / 1e-27
O170-O170_O4			104 / 2e-27
O124-WfeT	bDGalf(1->	->3)bDGalNAc	103 / 7e-27
O164-WfeT			103 / 7e-27
O85-WfbU	bDGalf(1->	->3)bDGlcNAc	98 / 4e-25
O167-WfdK	bDGalf(1->	->3)bDGlcNAc	95 / 5e-24
O96-O096_04			87 / 1e-21
O96-O096_08			84 / 2e-20

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfdK

bDGalf(1->

->3)bDGlcNAc

6 |

GT Name	Donor	Acceptor	Score / e-value
O85-WfbU	bDGalf(1->	->3)bDGlcNAc	309 / 8e-106
O170-O170_08			308 / 2e-105
O124-WfeT	bDGalf(1->	->3)bDGalNAc	307 / 7e-105
O164-WfeT			307 / 7e-105
O96-O096_08			267 / 4e-89
O42-WbeY	bDGalf(1->	->3)aDGlcNAc	234 / 2e-76
O28-WbeY	bDGalf(1->	->3)aDGlcNAc	233 / 5e-76
O170-O170_O4			163 / 5e-49
O167-WfdJ			95 / 4e-24
O96-O096_04			91 / 8e-23

ncbi CAZy

- Reference [1]

NMR
1H-NMR		5.23 4.16 4.23 4.06 3.86 3.70 3.70 5.03 4.03 4.07 4.15 3.94 3.73 3.73 5.01 4.09 4.00 3.62 3.88 3.85 3.92 2.08 8.47 4.90 4.02 4.23 3.83 3.80 3.66 3.70 4.68 3.82 3.66 3.48 3.44 3.76 3.91 2.05 8.21 4.65 3.59 3.75 4.25 4.21 1.35 7.65 4.16
Solvent:	D2O
Temp:	60C
pD:	5.8
Ref:	TSP 0.00 ppm
Comment:	1H-NMR [1], pD=5.8. Amid protons measured in H2O/D20 9:1 10C
13C-NMR		106.70 87.60 75.80 82.80 70.90 63.70 109.00 82.00 77.30 82.50 76.80 62.30 97.60 53.30 79.70 69.50 73.20 61.50 23.10 174.40 103.70 76.60 73.50 81.20 70.20 63.80 102.50 55.80 81.70 69.20 76.00 61.90 23.10 175.00 101.70 79.50 73.30 79.40 74.20 169.10 18.00 180.00 51.30
Solvent:	D2O
Temp:	60C
pD:
Ref:	Acetone 31.00 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- L-Alanine

Enzymes
Flippase		MVKTDLTVFKNIISLFSIRFAGYIIPLITLPYLVKVLQPVGFGYLSFGIAVLQYCTLVVNYGFDLSATRK IAQNRENVHYISDIFWNILFLRLFISLVGFLVLVVFIHLFPDLYRIKWILLWGYLSVLGTALFPMWLFQG KEQLGRISAARIFLQISCVPLFFVFVKNTQDDWVASLIYALPQLLISVVAVYIIHKRKWIVLKKIKLATI KKELIDGWHLFISTAAISLYTTTITVVLGIVPGPYSVAIYTSANKILQAAQGLYQPISSAFYPRINNLIK ENKQLAFKTIRKLFKIQFVITICISVGLFLFSNIVVDILYGDDYRNAALILQLLSPIPIVVGCSNIFGIQ ILLTCGYKKEFSTILLISGLINMILIFPLCYKFNEFGAAFSVLITEIFVTVSMLFVVILKKIPVWESKRN V
Trembl:	B5L3V7
Polymerase		MNNTLRQYSLVQVFITMYCLFPFYFHNIYIIQTFSLYSLALSFVVYTFFYKVRFVSRSGAITILASTTTF LIFLFMSMLINLSSDMTFVYEQVFGFSKQLLLALVPFCLYFSFDSDDINYAVHSILLLFIRAISLYVFIS VIFLIPEVRNYWIDIIYFSSDQNKELINSASYYTRFGLQGFSGFRHAILCAIALIFSIYLFMCGYKKIKN YWMSVFLVCIGCFLYGRIAIAVILCITLISLFYLLCKFEKLYFIFIVCLCVGTIACVVYMNIDYIQDSYS LAWIFEPLINFINEGQFTSHSTNDLSSMYIMPNENTMLLGDGKYTNHDGSYYMHTDVGFLRPVFFGGIFF VFFYYMMLILPLILIIKNSGLERRNILTLFLALALIVFELKGESFISFSAIIFSFILIPIICKRLN
Trembl:	B5L3W2

Literature
First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
First Author: Linnerborg, M. "Structural studies of the O-antigenic polysaccharide from Escherichia coli O167". European Journal of Biochemistry. 246(2). (1997), pp 565-573 PubMed: [9208951]
First Author: Gross, RJ. "Enterotoxigenic and enteroinvasive Escherichia coli strains belonging to a new O group, O167". European Journal of Biochemistry. 17(3). (1983), pp 521-523 PubMed: [6341404]

bDGalA6NLAla(1->

->3)aDGlcNAc

->3)aDGlcNAc

bDGalNAc(1->

->4)aDGal

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)aDGlcNAc

bDGlc(1->

->3)bDGalNAc

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)aDGlcNAc

bDGal(1->

->3)aDGalNAc

bDGalf(1->

->3)bDGlcNAc

bDGalf(1->

->3)bDGalNAc

bDGalf(1->

->3)bDGlcNAc

bDGalf(1->

->3)bDGlcNAc

bDGalf(1->

->3)bDGlcNAc

bDGalf(1->

->3)bDGlcNAc

bDGalf(1->

->3)bDGalNAc

bDGalf(1->

->3)aDGlcNAc

bDGalf(1->

->3)aDGlcNAc