O-Antigen Description

Serogroup:O6
Serotype H:1
Serotype K:2
Strain:DSM 6601
Structure:
->4)aDGalNAc(1->3)bDMan(1->4)bDMan(1->3)aDGlcNAc(1->
                     |
            bDGlc(1->2)
Comment:- Structure [1]
- Strain is given for gene sequence study and is for serotype O6:K5:H1 [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
Orf3
GT nameDonorAcceptorPosition | Link.NumSimilar to
Orf4
GT nameDonorAcceptorPosition | Link.NumSimilar to
Orf5

bDMan(1->

->4)bDMan

3 | 2
GT NameDonorAcceptorScore / e-value
O179-O179_07


152 / 9e-45
GT nameDonorAcceptorPosition | Link.NumSimilar to
Orf7

bDMan(1->

->3)aDGlcNAc

GT NameDonorAcceptorScore / e-value
O179-O179_08


350 / 2e-121
O106-WbaD


350 / 7e-121
O73-WbaD

bDMan(1->

->3)aDGlcNAc

350 / 7e-121
O77-WbaD

bDMan(1->

->3)aDGlcNAc

350 / 7e-121
O44-O044_03


345 / 2e-119
O17-O017_03


345 / 2e-119
O62-O062_12


322 / 3e-110
O59-WclC


214 / 3e-68
O176-O176_05


81 / 3e-19
NMR
1H-NMR4.80 3.98 3.81 3.84 3.55 3.86 3.74 4.91 4.13 4.01 3.68 4.17 3.84 3.77 5.32 4.30 4.06 4.09 4.29 3.79 3.73 4.81 4.37 3.85 3.84 3.46 3.95 3.80 4.68 3.38 3.49 3.44 3.42 3.87 3.74
Solvent: D2O
Temp: 55C
pD:
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [1]
13C-NMR101.60 71.50 72.80 78.10 76.20 62.20 99.40 54.10 81.80 69.50 73.10 61.80 100.30 51.20 68.50 78.60 73.70 61.50 101.80 78.90 77.90 68.00 77.90 62.20 104.70 74.90 76.80 70.80 77.40 61.60
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 31.45 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMNRIQRVHNYLRLKDKIKNVFLGLYILQLFNYIAPLVIIPILIKYIGLGEYGELVYITSI YQIVALIIDFGFTYTGPVVAARHRCETQNLQRYYSIVVLLKSLLFIIALTCVFLLCRLNI VHLSFFGFLSIFLCTIGNILSPNWFLQGIGDFKKLSYSQVIVRITLFIILLVYVCSGGDN VFILSFLQNATLLICCIYLWPNIHISHVVHLKPNECIVEFKKAGNVFIGVIGTIGYNGLI PVLIGNLCGNTSLGVFSIVQKMTTACQSLINPISQYMLSQVSEIKPQDKLFYYRIKKSFF VHLTISIIACLCYMGLGQYVATFIGKVDVSFVIILFASIITIFSSLNNVLGIQFLIPTDN VKILRSINVMAGIIVVSLSWLLISRFDILGGVLLNLIGEFLVFSMLAFIAHRKWGARV
Trembl: Q8KMW2
PolymeraseMKVKAVPAITFYLSLMLTILVLLFGNEPNKSQYILVIATITVFYIAYITNKITSPASLLV ISSFVFLGCRPLLSLFANYDYRIADWFIEGYMDDDVILANYAITLMYYGYTLGLILCKNT EKFYPHGPYPEKQLLKIKFLLTLFFLGSIGMVVKGIFFFNFIESNSYVDIYQSNITTPIG YDFLSYLFYCSFFLICAFHIQFRTNKKFLFIAICIAAFSTLKGSRSEAITFLLTVTCIYF NEVKTRNLRLLITMIFVFSVIFVISEFISMWRTGGSFFQLMQGNNPVINFVYGMGVSYLS IYQSVKLQLLSGGYNVTYLFSQLIITCSSIFNVKLSLPEISYSHLASYTANPELYNLGFG LGGSYLAESFLAFGLIGCFIIPFLLLLNLNVLEKYTKNKPIIYFVYYSVLPPILFTPRET LFYFFPYLVKSIFVAFLVTLYIQYKKD
Trembl: Q8KMV4
Literature
  • First Author: Jann, B. "Structural comparison of the O6 specific polysaccharides from E. coli O6:K2:H1, E. coli O6:K13:H1, and E. coli O6:K54:H10". Carbohydrate Research. 263(2). (1994), pp 217-225 PubMed: [7528640]
  • First Author: Grozdanov, L. "A single nucleotide exchange in the wzy gene is responsible for the semirough O6 lipopolysaccharide phenotype and serum sensitivity of Escherichia coli strain Nissle 1917". Journal of Bacteriology. 184(21. (2002), pp 5912-25 PubMed: [12374825]
  • First Author: Wu EL. "Molecular Dynamics and NMR Spectroscopy Studies of E. coli Lipopolysaccharide Structure and Dynamics". Biophysical Journal. 105(6). (2013), pp 1444-1455 PubMed: [24047996]