O-Antigen Description

Serogroup:O141
Serotype H:4
Serotype K:85
Strain:
Structure:
aLRha(1->3)
          |
   ->4)aDMan(1->3)aDMan6Ac(1->3)bDGlcNAc(1->
          |
bDGlcA(1->2)
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfcA
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfcB

GT NameDonorAcceptorScore / e-value
O25-WekA


261 / 3e-86
O68-O068_07


218 / 1e-69
O62-O062_09


218 / 1e-69
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfcC

aDMan(1->

->3)aDMan

GT NameDonorAcceptorScore / e-value
O88-O088_12


198 / 8e-62
O59-WclB


110 / 2e-29
O175-O175_05


96 / 3e-24
O64-O064_19


96 / 2e-24
O144-O144_05


94 / 1e-23
O9-MtfB

aDMan(1->

->3)aDMan

93 / 3e-23
O169-O169_06


92 / 3e-23
O183-wbaX


91 / 1e-22
O43-O043_08


86 / 1e-20
O7-WbbC

aDMan(1->

->4)bDGal

82 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfcD

aDMan(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O144-O144_04


350 / 6e-121
O115-WegL

aDMan(1->

->3)bDGlcNAc

313 / 8e-106
O58-WffE


305 / 5e-103
O78-WejO


301 / 3e-101
O88-O088_13


300 / 2e-101
O187-O187_13


261 / 5e-86
O34-O034_12


248 / 6e-81
O99-WejK

DRha(1->

->?)?DGlcNAc ->?)?DGalNAc

155 / 2e-45
O60-O060_12


154 / 4e-45
O175-O175_06


137 / 6e-39
O169-O169_07


136 / 2e-38
O183-wbaY


126 / 8e-35
O9-WbdC

aDMan(1->

->2)aDMan ->3)aDMan

112 / 4e-30
NMR
1H-NMR5.20 4.37 4.09 3.95 3.82 3.82 3.82 5.18 4.15 3.90 3.87 3.76 4.28 4.40 4.94 3.98 3.95 3.47 4.25 1.31 4.54 3.79 3.69 3.49 3.44 3.75 3.94 4.52 3.38 3.51 3.62 3.86 2.16 2.04
Solvent: D2O
Temp: 30C
pD: 3.2
Ref: TSP 0.00 ppm
Comment: 1H-NMR [2], pD=3.2
13C-NMR101.50 75.60 72.80 72.90 74.20 62.30 102.80 71.40 80.10 67.00 72.60 64.80 98.00 71.80 71.60 73.70 70.30 17.40 102.20 56.20 82.10 72.60 77.60 62.90 104.10 73.80 76.70 73.00 77.30 21.00 23.10 174.10 174.70 174.90
Solvent: D2O
Temp: 30C
pD: 3.2
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [2], pD=3.2
Non-Sugar Components
- Acetate
Enzymes
FlippaseMSKLKSNLVYLTFAQAISYIFPLLTLPYLSHKLDPSDFGMLGVCQAIVQYFIIVTDYGFNITTTKEISIN RNNKKKISKIFSTTLLAKIFLLFVSAIGLIISGWAVKSISGYELLLICCFVGVIGNTFYPLWLFQGTETM KIPVIMSSGAKLLLLLSIFIFVKDRADLNIAALLLNSGNFFAGILGLIYIKTRKLVFWYRPTLQEVYIVL LEGWPVFISTVAISFYTTFNTVLLSYKVSSAEVGFYNAADKIRIAIQSMFGPITQAFYPRIVHLYKVERE AAINLLNKGMLLILGLTIPSALLFFNCSDYIVAHYLSSSFSQTASYLKLLSFLPIIIGIATVYCNWGLLG DGYGKVVSKIYVSFGLIHFLYATPLIIIFKVNGLILSVYITQTLITLTTIYHYYRKVYNGFNK
Trembl: A6MEZ8
PolymeraseMEYMLLCFWLAISCCSVKVSLSKINILLIFIFLSGAYSWGYDWIRYREYYENVFLISDLVTWIYEPGFTF ILFVNKLLNLDYHWVVVTCTLILSVNVYKYIKVKNHPNFCLLFVFSIFGFMEFAEQIRQGVAISFILLAV DNLLVKKNKKFIFYVICAAMFHISAIVCLLFLYLVKLLDRKNKAFIMLSLIIWAVFLSLHLMFNIYRFFG VSGFIATKLKGYAESDGSESGILSAGLLLNIMVIVICLLSIQGRHLKYYLSSVFSFFVIQSKIVSIAYRF SYYGYAFIYESFEWLYMANKGRLFNKLAMIIVLLVFAFKPLLNPVYLDMFNDYHFFWFGFLDTLPDVNIT QVKRCSTLLNHGIQYCRR
Trembl: A6MF01
Literature
  • First Author: Han, W. "DNA microarray-based identification of serogroups and virulence gene patterns of Escherichia coli isolates associated with porcine postweaning diarrhea and edema disease". Applied and Environmental Microbiology. 73(12). (2007), pp 4082-4088 PubMed: [17449692]
  • First Author: Färnbäck, M. "Structural determination of the O-antigenic polysaccharide from Escherichia coli O141". European Journal of Biochemistry. 254. (1998), pp 168-171 PubMed: [9652410]