O-Antigen Description

Serogroup:O22
Serotype H:
Serotype K:13
Strain:
Structure:
->6)aDGlc(1->4)bDGlcA(1->4)bDGalNAc3Ac(1->3)aDGal(1->3)bDGalNAc(1->
Comment:- Structure [1]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbhP

aDGal(1->

->3)bDGalNAc

GT NameDonorAcceptorScore / e-value
O120-O120_15


273 / 3e-91
O87-WfgO


248 / 7e-81
O18-wekW


137 / 6e-39
O110-O110_02


135 / 2e-38
O113-WbnE


116 / 3e-31
O128-WbsH

aDGal(1->

->3)bDGalNAc

114 / 2e-30
O55-WbgM


108 / 1e-28
O55-WbgM

aDGal(1->

->3)bDGalNAc

107 / 2e-28
O142-O142_03


107 / 2e-28
O69-O069_12


107 / 2e-28
O86-WbnH


105 / 1e-27
O86-WbwH


105 / 1e-27
O86-WcmA

aDGalNAc(1->

->3)bDGalNAc

105 / 1e-27
O127-WbiN


105 / 1e-27
O90-O090_04


105 / 1e-27
O51-O051_12


104 / 2e-27
O76-O076_08


103 / 5e-27
O116-O116_10


99 / 2e-25
O3-WclR


95 / 4e-24
O89-O089_06


92 / 6e-23
O101-O101_06


91 / 9e-23
O142-O142_12


90 / 2e-22
O18-wekV

aDGlc(1->

->4)aDGal

89 / 6e-22
O148-WbbG

aDGlc(1->

->3)aDGlcNAc

85 / 2e-20
O40-WffO


84 / 4e-20
O19-O019_11


84 / 2e-20
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

81 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbhQ

bDGalNAc(1->

GT NameDonorAcceptorScore / e-value
O125-O125_04


105 / 4e-28
O5-O05_15


91 / 5e-23
O128-WbsK

bDGalNAc(1->

->4)aDGal

89 / 2e-22
O167-WfdH


84 / 8e-21
O112-WfeJ


82 / 5e-20
O64-O064_03


82 / 2e-20
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbhR

GT NameDonorAcceptorScore / e-value
O76-O076_07


108 / 6e-29
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbhT

GT NameDonorAcceptorScore / e-value
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

81 / 2e-19
NMR
1H-NMR5.39 3.43 3.64 3.37 3.76 4.02 3.82 4.43 3.42 3.70 3.75 3.78 4.75 4.17 5.03 4.21 3.71 3.75 3.75 5.06 3.87 3.78 4.15 3.82 3.72 3.72 4.53 4.03 3.80 4.12 3.63 3.70 3.70
Solvent: D2O
Temp: 80C
pD:
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [1]
13C-NMR99.30 73.00 74.10 70.50 72.00 69.10 104.40 74.70 77.40 78.00 77.60 174.30 103.90 52.40 74.20 74.30 75.50 61.90 96.60 68.30 80.90 70.20 72.20 62.30 102.90 52.10 76.80 65.40 76.00 62.10
Solvent: D2O
Temp: 80C
pD:
Ref: Acetone 31.45 ppm
Comment: 13C-NMR [1]
Non-Sugar Components
- Acetate
Enzymes
FlippaseMNNNIIDTAGRLKLIAFNFMIFVFLVRVPGFPFIILSIISITILSLFLIKVKVALNNKLIPLYCFVIYMI LRCVIGFVFNGGYEERYFMVLLLMLFVLIVFTVELKDVRLEQCYNGISAAVIVTLLIVLYEIISGNHMST SRYSDINNEAFLWSVSKPTAFYYNENDMLSFVVCFLPFTFVLKNTMLRVAIFLLAAASAIYIGSKAAIIT ILIYGLFTKIISKKAILLLVVTGVVLVNLFATSLIENTSSFISGSSERLLGFWDTILSTRGGDESTSERL SIYKANLDWLGNHPLSATFGNGRFGDYESELMKVYGLRMGEFHNLHLEMITIFGLLFYLLFFSYYFYLWI CLFLKKNKNTACYPLLISSAMYPLIISFGPSSSLKYPFLFILCLLMILGFRNDEKHAKKNI
Trembl: A7J0Q0
PolymeraseMKINDLLKKYTLFSLSPLLSAFIQLASIFLLGHSLSGPELGIAGIYNTIVFILVFISDGGSSSYFIYRKF LPKYDVNLINIFNVAISAFAIIIIGYYVPEKSDLLGLIVVSLTVTLPLYNYARLLVEKKYNIIAIVELKS KLLFFITLYIAINEFDYQGSAVVMSWAVSYFLRYCLFWWFSKKIELYKNDNLPQNKPAAILKSWWMYIHN QILSQVLNYFTITFDIFIISHFGGLEKVGVYSLCKDATLKISAVLSPVISKLLISHVVNIEENKISPMYK KVYKATIIISISVFGIWALCGSSIIHFIRPQLDQGILTFIIGWSICGILRMMINPIVSIFQATGKTRNEL YVNITSAVSFIFFMGMLSIYFDNIADAVILALICMYSISFVYATVLLRKYFSKIY
Trembl: A7J0Q3
Literature
  • First Author: Bartelt, M. "Structure of the O-specific polysaccharide of the O22-antigen (LPS) from Escherichia coli O22:K13". Carbohydrate Research. 254. (1994), pp 203-212 PubMed: [7514095]
  • First Author: Liu, Y. "Identifying Escherichia coli O22 and O91 serogroups by multiplex PCR of wzx and wzy gene of O-antigen gene cluster". Not specified. (2006),