O-Antigen Description

Serogroup:	O128 ab
Serotype H:
Serotype K:
Strain:
Structure:	->6)bDGal(1->3)bDGalNAc(1->4)aDGal(1->3)bDGalNAc(1-> \| aLFuc(1->2)
Comment:	- Structure [1] - Signals from O-acetylation at 2.16ppm and 2.18ppm in a 2:1 ratio have also been reported in [3]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsH

aDGal(1->

->3)bDGalNAc

1 | 1

GT Name	Donor	Acceptor	Score / e-value
O55-WbgM			372 / 4e-130
O55-WbgM	aDGal(1->	->3)bDGalNAc	371 / 1e-129
O110-O110_02			336 / 1e-115
O113-WbnE			254 / 2e-83
O69-O069_12			244 / 9e-80
O3-WclR			243 / 4e-79
O18-wekW			228 / 2e-73
O40-WffO			215 / 2e-68
O19-O019_11			164 / 5e-49
O148-WbbG	aDGlc(1->	->3)aDGlcNAc	160 / 1e-47
O51-O051_12			152 / 2e-44
O87-WfgO			145 / 5e-42
O120-O120_15			138 / 3e-39
O22-WbhP			114 / 2e-30
O142-O142_12			103 / 3e-27
O76-O076_08			94 / 1e-23
O89-O089_06			88 / 9e-22
O142-O142_03			84 / 2e-20
O101-O101_06			83 / 8e-20
O111-WbdM	aCol(1->	->3)aDGlc ->6)aDGlc	82 / 1e-19
O86-WbnH			81 / 3e-19
O86-WbwH			81 / 3e-19
O86-WcmA	aDGalNAc(1->	->3)bDGalNAc	81 / 4e-19
O127-WbiN			81 / 3e-19
O90-O090_04			81 / 3e-19

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsJ

aLFuc(1->

->2)bDGal

2 | 4

GT Name	Donor	Acceptor	Score / e-value
O55-WbgN			157 / 4e-47
O55-WbgN	aCol(1->	->2)bDGal	157 / 4e-47
O86-WbnK	aLFuc(1->	->2)bDGal	145 / 1e-42
O86-WbwK	aLFuc(1->	->2)bDGal	145 / 2e-42
O86-WcmD	aLFuc(1->	->2)bDGal	145 / 1e-42
O126-WbgL			140 / 1e-40
O127-WbiQ			123 / 2e-34
O90-O090_14			123 / 2e-34

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsK

bDGalNAc(1->

->4)aDGal

3 | 2

GT Name	Donor	Acceptor	Score / e-value
O125-O125_04			150 / 3e-44
O55-WbgO			134 / 2e-38
O55-WbgO			134 / 2e-38
O113-WbnA			134 / 2e-38
O167-WfdH			133 / 6e-38
O86-WbnJ	bDGal(1->	->3)aDGalNAc	126 / 6e-36
O86-WcmC	bDGal(1->	->3)aDGalNAc	126 / 8e-36
O86-WbwJ			125 / 2e-35
O46-O046_08			120 / 1e-33
O134-O134_08			120 / 1e-33
O127-WbiP			117 / 1e-32
O90-O090_13			117 / 1e-32
O173-WcmW	bDGlc(1->	->3)bDGlcNAc	113 / 7e-31
O23-O023_06			112 / 8e-31
O48-O048_06			110 / 1e-29
O102-WelM	bDGalNAc(1->	->3)aDGal	107 / 2e-28
O27-WejU			107 / 8e-29
O157-WbdN			105 / 5e-28
O74-O074_09			104 / 7e-28
O61-WeiL			102 / 2e-27
O165-WcnW			102 / 2e-27
O24-WfaM	bDGlc(1->	->3)bDGalNAc	99 / 1e-25
O54-O054_06			99 / 9e-26
O81-O081_11			99 / 8e-26
O130-WffJ			97 / 2e-25
O130-WffH'			97 / 8e-25
O91-WbsA			97 / 2e-25
O112-WfeI			96 / 7e-25
O21-WclQ			95 / 2e-24
O37-O037_11			95 / 2e-24
O82-O082_09			94 / 3e-24
O152-WfgD	bDGlc(1->	->3)bDGlcNAc	94 / 3e-24
O102-WelO	bDGlc(1->	->3)bDGalNAc	94 / 3e-24
O30-O030_06			94 / 3e-24
O126-WbsT			93 / 8e-24
O71-WdaK	bDGal(1->	->3)aDGalNAc	93 / 1e-23
O93-O093_02			91 / 5e-23
O64-O064_03			90 / 1e-22
O143-WfdV			89 / 2e-22
O22-WbhQ			89 / 2e-22
O38-O038_09			88 / 3e-22
O117-WbeA			86 / 2e-21
O185-O185_03			86 / 4e-21
O107-WclW			85 / 4e-21
O63-WbuP			84 / 1e-20
O70-O070_09			84 / 1e-20
O92-O092_07			84 / 1e-20
O146-WbwW			81 / 1e-19
O182-O182_10			81 / 8e-20
O140-O140_14			81 / 6e-20
O43-O043_04			80 / 2e-19

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbsL

bDGal(1->

->3)bDGalNAc

4 | 3

GT Name	Donor	Acceptor	Score / e-value
O76-O076_03			132 / 2e-37

ncbi CAZy

- Reference [2]

NMR
1H-NMR		4.53 3.99 3.90 4.05 3.68 3.80 3.80 5.08 3.68 3.87 4.11 3.82 4.59 4.03 3.81 4.15 3.66 3.80 3.80 4.61 3.65 3.81 3.90 3.77 3.79 3.79 5.24 3.78 3.66 3.73 4.22 1.21 2.05 2.05
Solvent:	D2O
Temp:	70C
pD:
Ref:	Acetone 2.225 ppm
Comment:	1H-NMR [1]
13C-NMR		104.70 52.80 78.20 69.60 75.90 62.40 96.90 69.60 70.50 78.00 71.90 61.50 102.60 51.90 77.20 65.40 76.10 62.40 103.30 77.30 74.90 70.00 74.20 69.30 100.40 69.50 70.90 73.10 68.00 16.50 23.60 23.80 175.25 175.35
Solvent:	D2O
Temp:	70C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [1]

Enzymes
Flippase		MNKKEIIILLIARILQILISLFTMRLMTTILSNEQMGEYYIFLAIYMLASLGIINPIGQY VNRNTYLWWREGVLTQYINRYINFVLCFSLIYVILYGLGECLFKGINTPFWMLFCYLYIV CLSLNQFLLHTLNILNFKIVFAITTLMTSVLSLSGAYLFIEYSIAKQTVSIYYWVAGIII GNFISLCICVVYLRSKNTFQKSILKSINVSIKEVFNFCCPIMITTLFIWFQSSGYRFAVE QMFGLKFLGILAVCFAVSGQIMSVIESLVTQIFQPTLFKHMDNVKNRKQHLDFYINENIA IYASVLFFCTFFMREIFLVLVDNKYLPYFYIGIIALWSEFFRISTNTLSLVFFCEKKMKV GIFPYLIGSLSLAFSIVLFRLLNVNLGILIAFAIGVAQLASFIVCLITVKKISYFKINSI FLMKRIIIVLPSVLIAHCIPKFEMINAIHFIIIFGVGLFYLISLATSFMFIKQPGGVYGS
Trembl:	Q6XQ54
Polymerase		MISYGVLFLFSYINAVLQKRVTVTLLFFIMIIVSFFKYNIGFDWGNYHEIYLQQGDGFFF YEPLFSLLIILSNKVALEYKYFISMCSLITLIVYYSGMRCFARDIKELALALTYFVVSYC YFEHFNIIRQTLAIALFFYSWKYIIETNFIMYSLFIFFASMIHAAAVVLLPFYFFLRIDN FKPVYYGIVVLFIIIVVRFDINSILLSSLNLFKNFDFVARYLANSTDLADFASKKSDLGL SKMYPAIIFVFCCFWGGIRLESRRDYVFFNAGFIAITLLLLSYQVDILFRFFSFFETSFV LLYCIIFSGFNKKSKIILHMVNLIVYTVMTFNMLSVMGLIPYESYIF
Trembl:	Q6XQ50

Literature
First Author: Sengupta, P. "Structure of the O-specific side chain of the Escherichia coli O128 lipopolysaccharide". Carbohydrate Research. 277. (1995), pp 283-290 PubMed: [8556736]
First Author: Shao, J. "Sequence of Escherichia coli O128 antigen biosynthesis cluster and functional identification of an alpha-1,2-fucosyltransferase". FEBS Letters . 553. (2003), pp 99-103 PubMed: [14550554]
First Author: Ali T. "Structural studies of the O-antigenic polysaccharides from the enteroaggregative Escherichia coli strain 87/D2 and international type strains from E. coli O128". Carbohydrate Research. 343. (2008), pp 695-702 PubMed: [18237721]

aDGal(1->

->3)bDGalNAc

aDGal(1->

->3)bDGalNAc

aDGlc(1->

->3)aDGlcNAc

aCol(1->

->3)aDGlc ->6)aDGlc

aDGalNAc(1->

->3)bDGalNAc

aLFuc(1->

->2)bDGal

aCol(1->

->2)bDGal

aLFuc(1->

->2)bDGal

aLFuc(1->

->2)bDGal

aLFuc(1->

->2)bDGal

bDGalNAc(1->

->4)aDGal

bDGal(1->

->3)aDGalNAc

bDGal(1->

->3)aDGalNAc

bDGlc(1->

->3)bDGlcNAc

bDGalNAc(1->

->3)aDGal

bDGlc(1->

->3)bDGalNAc

bDGlc(1->

->3)bDGlcNAc

bDGlc(1->

->3)bDGalNAc

bDGal(1->

->3)aDGalNAc