O-Antigen Description

Serogroup:O35
Serotype H:10
Serotype K:
Strain:
Structure:
       ->3)aLRha(1->2)aLRha(1->3)aLRha(1->2)aLRha(1->3)bDGlcNAc(1->
              |
aDGalNAcAN(1->2)
Comment:- Structure [1]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbpS
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcnX

GT NameDonorAcceptorScore / e-value
O105-WbsV


126 / 3e-36
O54-O054_07


126 / 3e-36
O120-O120_13


122 / 1e-34
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcnY

aLRha(1->

GT NameDonorAcceptorScore / e-value
O120-O120_14


145 / 1e-42
O10-O010_09


142 / 4e-41
O146-WbwV


140 / 1e-40
O129-RfbF


140 / 1e-40
O135-RfbF


140 / 8e-41
O13-RfbF


140 / 1e-40
O19-O019_08


139 / 4e-40
O69-O069_11


138 / 8e-40
O19-O019_09


136 / 3e-39
O148-WbbQ


135 / 8e-39
O147-WfbY


135 / 6e-39
O54-O054_10


125 / 3e-35
O150-WfgI


122 / 4e-34
O133-O133_07

aLRha(1->

->2)aLRha
->4)aDGalA

114 / 3e-31
O71-WdaM

aLRha(1->

->4)bDGal

99 / 1e-25
O139-WfaI


93 / 1e-23
O177-WbdY


91 / 4e-23
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcnZ

aLRha(1->

GT NameDonorAcceptorScore / e-value
O119-WekH

aLRha(1->

->3)aDGlcNAc

222 / 6e-73
O48-O048_08


220 / 3e-72
O139-WfaL

aLRha(1->

->3)aDGlcNAc

181 / 6e-57
O75-wekZ


180 / 1e-56
O16-WbbL

aLRha(1->

->3)aDGlcNAc

171 / 4e-53
O39-O039_13


156 / 2e-47
O132-O132_08


135 / 2e-39
NMR
1H-NMR4.69 3.71 3.59 3.32 3.41 3.66 3.90 2.06 4.95 4.35 3.91 3.54 3.71 1.27 5.15 4.05 3.94 3.44 3.81 1.29 4.86 4.11 3.82 3.54 3.76 1.27 5.01 3.78 3.87 3.45 3.99 1.24 5.12 4.16 4.00 4.35 4.82 2.03
Solvent: D2O
Temp: 50C
pD: 3.2
Ref: Not Stated NULL ppm
Comment: 1H-NMR [1], pD=3.2. Amide protons at 8.08, 7.80, 7.27 and 7.58
13C-NMR103.40 56.70 82.30 70.00 76.40 62.00 23.40 175.10 99.50 75.30 79.20 72.20 70.30 17.50 101.70 78.40 70.60 72.90 69.70 17.50 103.10 70.60 78.40 72.10 69.70 17.50 100.60 80.60 70.60 73.10 69.70 17.50 95.60 50.00 67.80 69.50 72.00 174.60 22.80 175.40
Solvent: D2O
Temp: 50C
pD:
Ref: Not Stated NULL ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMYLKILIDNISLVIQYFFGGIAVFYVTPLIVKSVGIHTYGNLAIMFAIVTYISVVIQYSF NLIGPKLIAEGNCKKTFNTIISAKIVLFLLSLIFFLLYFYFFQNKHDDVFLLFLVFPLSW VFNSAWYLQSKGYFVISSVCSIIGSLITFVIAYFFINTTTGLFVPILCLVLSSFINGLLT FFFAVKNNGHIEIVNPLPMLKEGMPLFVSQIISSLYTMSGVFIITYFYGVASAGTYAIVE RFMNLLISLGVLTHVAAYPKLARLFNKDRLEYRKTLLFVIALYTLFSCCVAIVVFNFHQN IVTYMFGSESVNAKELIYSACIFIFVSIYGPVVTGYFTLKVKGRMIITINIIIALLSLLL GVGMLKIVGSSGWLIGLSLAQLLYIAIFFKIFIWGRKECAV
Trembl: D6BV45
PolymeraseMNGVAKPIIISWFVLCTLVVFFLCSFEQFPDYYSYLNWYELSVSATLNNNWVFFKDPGFY LLSVISNNFDFGIIGAIFFLFIISLSCKIFFCIKLLDWEVLFWVLLLYLSRLFIIHDLVQ YRAGASIGLSALFVYFYLEKKRIKSFFFLSLALSIHLSSLLMVSVIPIAWFLNRNVGGNI IRQIAILLLILSLSLFFDPYVNLLRLVSQFPLMHERIAPYLDGSYLVSNTSMFNSFVIIK IISYVIFFVWICKNKNVGLTNENYLIYLCFFISIVGLFLFWCFRSNDSLSIRFSDFFALY DIVFFALLLNVFDVFGKFIYRYCLLVVVIVFFISSMKLIN
Trembl: D6BV49
Literature
  • First Author: Rundlöf, T. "Structural determination of the O-antigenic polysaccharide from Escherichia coli O35 and cross-reactivity to Salmonella arizonae O62". European Journal of Biochemistry. 258(1). (1998), pp 139-143 PubMed: [9851702]
  • First Author: Liu B. "Development of a serogroup-specific DNA microarray for identification of Escherichia coli strains associated with bovine septicemia and diarrhea". Veterinary Microbiology. 142. (2010), pp 373-378 PubMed: [19932572]