O-Antigen Description

Serogroup:O86
Serotype H:2
Serotype K:62
Strain:
Structure:
         aDGal(1->3)
                  |
->4)aLFuc(1->2)bDGal(1->3)aDGalNAc(1->3)bDGalNAc(1->
Comment:- Structure [1]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnH

aDGalNAc(1->

->3)bDGalNAc

GT NameDonorAcceptorScore / e-value
O86-WcmA

aDGalNAc(1->

->3)bDGalNAc

655 / 0.0
O86-WbwH


654 / 0.0
O127-WbiN


654 / 0.0
O90-O090_04


650 / 0.0
O142-O142_03


358 / 3e-124
O76-O076_08


282 / 9e-95
O120-O120_15


128 / 1e-35
O87-WfgO


111 / 1e-29
O22-WbhP


105 / 1e-27
O69-O069_12


85 / 1e-20
O55-WbgM


82 / 9e-20
O128-WbsH

aDGal(1->

->3)bDGalNAc

81 / 3e-19
O110-O110_02


81 / 3e-19
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnI

aDGal(1->

->3)bDGal

2 | 3/4
GT NameDonorAcceptorScore / e-value
O86-WbwI

aDGal(1->

->3)bDGal

479 / 0.0
O86-WcmB

aDGal(1->

->3)bDGal

476 / 0.0
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnJ

bDGal(1->

->3)aDGalNAc

3 | 2
GT NameDonorAcceptorScore / e-value
O86-WbwJ


521 / 0.0
O86-WcmC

bDGal(1->

->3)aDGalNAc

511 / 0.0
O127-WbiP


305 / 1e-105
O90-O090_13


305 / 1e-105
O23-O023_06


236 / 2e-78
O126-WbsT


148 / 1e-44
O55-WbgO


135 / 2e-39
O55-WbgO


135 / 2e-39
O167-WfdH


129 / 9e-37
O128-WbsK

bDGalNAc(1->

->4)aDGal

126 / 5e-36
O113-WbnA


105 / 2e-28
O54-O054_06


91 / 2e-23
O48-O048_06


89 / 1e-22
O130-WffH'


83 / 2e-20
O171-O171_13


82 / 4e-20
O125-O125_04


82 / 6e-20
O37-O037_11


82 / 3e-20
O74-O074_09


81 / 1e-19
O146-WbwW


81 / 1e-19
O178-O178_09


81 / 6e-20
O5-O05_15


81 / 9e-20
O153-O153_06


81 / 6e-20
O104-WbwC

bDGal(1->

->3)bDGalNAc

81 / 6e-20
O65-O065_13


81 / 8e-20
O71-WdaK

bDGal(1->

->3)aDGalNAc

81 / 7e-20
O185-O185_03


81 / 1e-19
O63-WbuP


80 / 1e-19
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnK

aLFuc(1->

->2)bDGal

4 | 3/4
GT NameDonorAcceptorScore / e-value
O86-WcmD

aLFuc(1->

->2)bDGal

629 / 0.0
O86-WbwK

aLFuc(1->

->2)bDGal

628 / 0.0
O127-WbiQ


293 / 2e-99
O90-O090_14


293 / 2e-99
O128-WbsJ

aLFuc(1->

->2)bDGal

145 / 1e-42
O55-WbgN


119 / 5e-33
O55-WbgN

aCol(1->

->2)bDGal

119 / 5e-33
O126-WbgL


112 / 4e-30
ncbi CAZy
- Reference [2]
NMR
1H-NMR5.22 3.89 3.89 3.98 4.20 3.73 4.68 3.89 3.95 4.27 3.65 3.78 5.04 4.23 3.94 4.21 3.86 3.76 4.56 4.14 3.78 4.06 3.66 3.78 5.22 3.78 3.54 3.78 4.33 1.21
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 2.212 ppm
Comment: 1H-NMR [1]
13C-NMR94.30 69.00 70.40 70.30 72.00 62.20 103.20 73.90 75.50 64.70 75.30 61.80 94.10 49.60 77.40 69.80 71.80 61.70 103.60 51.90 75.60 64.50 76.00 61.80 99.70 69.30 70.30 83.40 67.80 16.20
Solvent: D2O
Temp: 70C
pD:
Ref: Dioxane 67.4 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMIKLLFSSSLLKMGVVLLNLLIPAIIIRFYSEYDFGIYSYLLTYAIVISVLQNGITASFRNLISNLEKND LLDYLLYSVRKITYPFLLIIFCLLLATLYVVGFDTLYGKICFFIAVSLINLYYPVVASYYDATGKTLNFV LLEIIFLLVFIAGVGILIYYKVSIYIVCILTANYRGVYAILLIMKKCVIFKQIRLKKKGGYKIFCSDDMI FIIIQLINVSNTLLFMNMFAKFYGVVAFGVFSVYYRFVAFPQQVISFSSSLIWINFRQVHVNNRVAAMQL LKRLFFIFTLLLIIWGGCVHFFIDKVVYLYSSKPLEYPGVLCFLNIMVCLMLLKDFSSIILNALTLYKEQ MIMNALLCLLNVIFFFFYNETNFDTIYLIFVSLLTLMFVFVNLSLIRSRL
Trembl: Q4KXD2
PolymeraseMVKIKTPRLLCVIRKEKVGMVISRSNYRNICSYTFFMVNLFILILSVINEGFCEIAYVIISVSSVLFCVI IICLERQGGFLNPMTFCIISVFFFILIRPVFFSQNITENLNEVITAGLEIDEIYVFYSLAVVNISLAFTV LLYSVQKGTVSKLVGQLPDLFFYNKQLSMILLWGGGLFSAIFLIKSYKKFIILGQVSVFEADAYGLHDEL FWFTLSKYCYILSLLFSKNKNFILYSLLIFITSIGYILVGLRGYTIAYGFLLLFFLDIRYRLKIKWLLLV AILVTTISSLILNYRIGIEVNSGLLGIIFNPLLQQGASFETVYGALKYNEKILSCISYYDYFFTNKDIGS CIDIARGVYFKEGGSFASSFYSELIYLGWIIGSVALLLFAFSLAFVQSCYEKIIKNSMNNKLAYTYRLII FLALPNLIYFARSSLFDFITKVLFIALFIGGLSIVRHIALNIKKCH
Trembl: Q4KXD0
Literature
  • First Author: Andersson, M. "Structural studies of the O-antigenic polysaccharide of Escherichia coli O86, which possesses blood-group B activity". Carbohydrate Research. 185(2). (1989), pp 211-223 PubMed: [2471591]
  • First Author: Yi, W. "Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide". J American Chemical Society. 127(7). (2005), pp 2040-1 PubMed: [15713070]