O-Antigen Description

Serogroup:	O173
Serotype H:	H-
Serotype K:	K-
Strain:	CCUG 36541
Structure:	aLFuc(1->4) \| ->3)aDGlc(1->P-6)aDGlc(1->2)bDGlc(1->3)bDGlcNAc(1->
Comment:	- Structure [1].

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WcmU

aLFuc(->

->4)aDGlc

1 | 4

GT Name	Donor	Acceptor	Score / e-value
O41-O041_06			123 / 3e-35
O43-O043_13			122 / 2e-34
O11-WdaG			119 / 1e-33
O125-O125_13			119 / 9e-34
O43-O043_04			119 / 9e-34
O11-WdaD			117 / 4e-33
O36-O036_08			117 / 1e-32
O168-WfeY			108 / 1e-29
O184-O184_15			108 / 2e-29
O156-O156_14			108 / 1e-29
O41-O041_12			107 / 5e-29
O84-O084_13			107 / 2e-29
O36-O036_15			105 / 2e-28
O159-WfgS			103 / 6e-28
O168-WfeV			98 / 1e-25
O157-WbdO			90 / 5e-23
O111-WbdL	aDGlc(1->	->4)aDGal	86 / 2e-21
O159-WfgP			81 / 8e-20

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WcmV

aDGlc(1->

P->6)aDGlc

2 | 3

GT Name	Donor	Acceptor	Score / e-value
O152-WfgC			344 / 3e-118
O160-O160_07			196 / 5e-61
O181-O181_03			145 / 6e-42

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WcmW

bDGlc(1->

->3)bDGlcNAc

3 | 1

GT Name	Donor	Acceptor	Score / e-value
O152-WfgD	bDGlc(1->	->3)bDGlcNAc	235 / 4e-78
O165-WcnW			209 / 5e-68
O82-O082_09			192 / 1e-61
O56-WfaP	bDGlc(1->	->3)bDGlcNAc	176 / 3e-55
O38-O038_09			147 / 3e-44
O112-WfeI			145 / 3e-43
O61-WeiL			139 / 4e-41
O93-O093_02			138 / 1e-40
O130-WffJ			138 / 1e-40
O143-WfdV			138 / 2e-40
O140-O140_14			137 / 3e-40
O46-O046_08			136 / 5e-40
O182-O182_10			135 / 1e-39
O134-O134_08			131 / 5e-38
O102-WelO	bDGlc(1->	->3)bDGalNAc	126 / 3e-36
O21-WclQ			124 / 3e-35
O30-O030_06			123 / 6e-35
O161-weiG	bDGlcA(1->	->3)bDGlcNAc	122 / 9e-35
O27-WejU			122 / 7e-35
O81-O081_11			118 / 7e-33
O79-WbdX			115 / 3e-32
O91-WbsA			115 / 3e-32
O128-WbsK	bDGalNAc(1->	->4)aDGal	113 / 6e-31
O157-WbdN			111 / 1e-30
O24-WfaM	bDGlc(1->	->3)bDGalNAc	108 / 1e-29
O55-WbgP			102 / 3e-27
O55-WbgP			101 / 4e-27
O113-WbnA			92 / 2e-23
O102-WelM	bDGalNAc(1->	->3)aDGal	87 / 6e-22
O12-O012_O2			87 / 1e-21
O48-O048_06			86 / 2e-21
O46-O046_05			86 / 3e-21
O134-O134_05			86 / 3e-21
O51-O051_11			86 / 2e-21
O111-WbdL	aDGlc(1->	->4)aDGal	84 / 7e-21
O55-WbgO			83 / 1e-20
O32-WfeA			83 / 3e-20
O130-WffH'			83 / 2e-20
O55-WbgO			83 / 1e-20
O117-WbeA			83 / 2e-20
O125-O125_04			83 / 3e-20
O107-WclW			81 / 6e-20

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WcmX

aDGlc(1->

->2)bDGlc

4 | 2

GT Name	Donor	Acceptor	Score / e-value
O152-WfgE			325 / 1e-110
O24-WfaO	aDGlc(1->	->2)bDGlc	324 / 2e-110
O56-WfaQ			263 / 2e-86
O29-WffY			177 / 1e-53
O81-O081_10			160 / 2e-47
O18-wcxD			151 / 4e-44
O85-WfbQ			146 / 8e-42
O130-WffI			137 / 2e-38
O144-O144_03			131 / 1e-36
O102-WelN	aDGal(1->	->6)bDGlc	123 / 8e-34
O187-O187_10			121 / 6e-33
O27-WejX			120 / 2e-32
O169-O169_04			117 / 9e-32
O183-wbaT			115 / 6e-31
O66-WfaB			103 / 9e-27
O34-O034_11			102 / 2e-26
O110-O110_03			92 / 5e-23
O65-O065_10			89 / 6e-22
O101-O101_06			82 / 2e-19
O178-O178_05			81 / 4e-19
O153-O153_02			81 / 4e-19
O89-O089_06			80 / 9e-19

ncbi CAZy

- Reference [2]

NMR
1H-NMR		5.44 3.63 4.03 3.68 3.91 3.86 3.86 5.24 3.52 3.73 4.06 3.51 4.06 4.12 4.97 3.76 3.86 3.77 4.84 1.25 4.93 3.78 3.99 3.39 3.42 3.61 3.93 4.68 3.42 3.53 3.43 3.40 3.70 3.89 2.06
Solvent:	D2O
Temp:	50C
pD:	8
Ref:	TSP 0.00 ppm
Comment:	1H-NMR [1], pD=8
13C-NMR		96.10 73.40 77.90 72.80 73.20 60.40 98.80 72.60 73.50 71.70 69.80 65.10 99.20 68.80 70.20 72.90 67.40 16.30 101.80 56.40 80.00 70.10 76.10 62.60 102.00 78.80 75.70 70.60 76.70 61.40
Solvent:	D2O
Temp:	50C
pD:	8
Ref:	Acetone 31.00 ppm
Comment:	13C-NMR [1], pD=8

Non-Sugar Components
- Phosphate

Enzymes
Flippase		MNKKVLLNIFSLLSIQGVGYILPLITLPYLVRILGPNNYGVYGYSLAIIQYSILIIDFGFNLTVTKKISL NRDDKLIVSSVFWNVMACKLLLCIFTFLITVAWSRIDNTFAGDNLAVLMSSYTMVIGILIFPIWLFQGKE EITKIALINISSKFLTVPLIFILIDKPSDGWKVGLINGSGYIVAGIIGLHIVSRRNWIVWVKPTFSQMFR EYASAWHIFISNASINIYTSSITVILGALTSPVTVGYFVAADKIRLAFQGLINPVSQALYPRINALMKES PAAGFKTIRLLLRTQGVFFFFCSGFIFYLSPNIISFLYGESYKESIPILKILAWLPFIIAISNVLGYQTL LVLNMERIFSRLVLVGGGIALVIIFPLIIYFGGYGAALTILIAEVSVNL
Trembl:	E2DNQ8
Polymerase		MLETYFIYNFIMLCGIFFAFVKENTHSIIFKVASSLMLIFVIWIPSGIRYGIGTDYFSYLRIFQSALEGR VTTEIGYYLINYLVVVTGGEGYYIFLISSLIIALVIVINMPRHYSCLYVLIFFCIIYLPSFSLVRQSLAV VFLLVSIKHAIENKILLSYFFALVAISFHLSSLLILPFIILRKVRVPPLLALCMIVFCIIFFLSTNILQD IINSSFIASTKYGFYADTKFGGEAIIGTGGGVLIKALLPLMIILQSSKIVKSQPNCNIIILLSIVAICAS IASLRIHIFSRVSDLFMFLPLLGVTYLMNSFSKKITKQICACLIFLSMIVLFERTIATNLSENNSGLGVS PYTTIFDVR
Trembl:	E2DNR6

Literature
First Author: Linnerborg, M. "Structural studies of the O-antigen polysaccharide from the enteroinvasive Escherichia coli O173". Carbohydrate Research. 320. (1999), pp 200-208 PubMed: [10573858]
First Author: Quan Wang. "Development of a serogroup-specific multiplex PCR assay to detect a set of Escherichia coli serogroups based on the identification of their O-antigen gene clusters". Molecular and Cellular Probes. 24. (2010), pp 286-290 PubMed: [20561581]

aLFuc(->

->4)aDGlc

aDGlc(1->

->4)aDGal

aDGlc(1->

P->6)aDGlc

bDGlc(1->

->3)bDGlcNAc

bDGlc(1->

->3)bDGlcNAc

bDGlc(1->

->3)bDGlcNAc

bDGlc(1->

->3)bDGalNAc

bDGlcA(1->

->3)bDGlcNAc

bDGalNAc(1->

->4)aDGal

bDGlc(1->

->3)bDGalNAc

bDGalNAc(1->

->3)aDGal

aDGlc(1->

->4)aDGal

aDGlc(1->

->2)bDGlc

aDGlc(1->

->2)bDGlc