O-Antigen Description

Serogroup:	O65
Serotype H:
Serotype K:
Strain:
Structure:	->2)bDQui3NAc(1->4)aDGalA6N(1->4)aDGalNAc(1->4)bDGalA(1->3)aDGlcNAc(1->
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O065_08

GT Name	Donor	Acceptor	Score / e-value
O121-WbqE			106 / 4e-28
O125-O125_04			97 / 6e-25
O5-O05_12			92 / 3e-23
O116-O116_07			83 / 3e-20
O103-WbtE			80 / 3e-19

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O065_10

GT Name	Donor	Acceptor	Score / e-value
O110-O110_03			186 / 2e-57
O27-WejX			112 / 9e-30
O178-O178_05			108 / 8e-29
O153-O153_02			108 / 8e-29
O24-WfaO	aDGlc(1->	->2)bDGlc	101 / 2e-26
O152-WfgE			101 / 3e-26
O85-WfbQ			99 / 2e-25
O81-O081_10			92 / 3e-23
O173-WcmX	aDGlc(1->	->2)bDGlc	91 / 8e-23
O29-WffY			88 / 1e-21
O102-WelN	aDGal(1->	->6)bDGlc	86 / 4e-21
O183-wbaT			85 / 1e-20
O130-WffI			84 / 5e-20
O169-O169_04			84 / 3e-20
O34-O034_11			84 / 3e-20
O104-WbwB	aDGal(1->	->4)aNeu	83 / 8e-20
O66-WfaB			82 / 2e-19
O56-WfaQ			82 / 2e-19

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O065_12

GT Name	Donor	Acceptor	Score / e-value
O75-wekY			149 / 4e-43
O89-O089_06			94 / 1e-23
O101-O101_06			92 / 3e-23
O74-O074_11			82 / 1e-19
O103-WbtF			81 / 3e-19
O18-wekW			81 / 3e-19

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O065_13

->3)aDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O178-O178_09			192 / 4e-61
O153-O153_06			192 / 4e-61
O71-WdaK	bDGal(1->	->3)aDGalNAc	187 / 3e-59
O5-O05_15			184 / 6e-58
O174-WbcZ			178 / 2e-55
O107-WclZ	bDGal(1->	->3)aDGalNAc	177 / 2e-55
O104-WbwC	bDGal(1->	->3)bDGalNAc	177 / 3e-55
O117-WbeD	bDGal(1->	->3)aDGalNAc	177 / 4e-55
O33-O033_09			173 / 1e-53
O171-O171_13			171 / 1e-52
O7-WbbD	bDGal(1->	->3)aDGlcNAc	162 / 2e-49
O146-WbwW			161 / 4e-49
O185-O185_03			158 / 8e-48
O112-WfeJ			156 / 3e-47
O160-O160_08			154 / 2e-46
O70-O070_13			149 / 2e-44
O155-WclG			147 / 7e-44
O37-O037_13			147 / 7e-44
O131-O131_11			137 / 7e-40
O63-WbuP			124 / 3e-35
O28-O028ab_02			123 / 1e-34
O54-O054_06			120 / 8e-34
O74-O074_09			115 / 9e-32
O64-O064_03			108 / 3e-29
O127-WbiP			95 / 1e-24
O90-O090_13			95 / 1e-24
O55-WbgO			91 / 3e-23
O55-WbgO			91 / 3e-23
O167-WfdH			91 / 4e-23
O114-WbuP	bDGal(1->	->3)aDGlcNAc	86 / 2e-21
O86-WbnJ	bDGal(1->	->3)aDGalNAc	81 / 9e-20
O86-WcmC	bDGal(1->	->3)aDGalNAc	81 / 1e-19

ncbi CAZy

- Reference [2]

NMR
1H-NMR		5.72 4.11 3.62 3.62 3.62 3.92 3.85 5.08 3.77 4.08 4.51 4.80 4.99 4.18 4.04 4.15 4.40 3.84 3.82 4.85 3.41 3.87 3.18 3.44 1.27 4.43 3.58 3.82 4.41 4.46
Solvent:	D2O
Temp:	32C
pD:	2.8
Ref:	Acetone 2.225 ppm
Comment:	1H-NMR [1], pD=2.8
13C-NMR		95.52 52.43 83.21 69.14 73.22 61.42 100.71 69.62 70.38 77.20 71.55 174.40 99.24 50.55 67.28 77.67 71.88 60.27 103.76 73.73 56.44 73.72 73.73 17.55 104.43 70.67 72.47 77.86 74.22 171.48
Solvent:	D2O
Temp:	32C
pD:	2.8
Ref:	Acetone 31.07 ppm
Comment:	13C-NMR [1], pD=2.8

Literature
First Author: Perry, MB. "Structural characterization of the antigenic O-chain of the lipopolysaccharide of Escherichia coli serotype O65". Carbohydrate Research. 322. (1999), pp 57-66 PubMed: [10629949]