O-Antigen Description

Serogroup:O164
Serotype H:
Serotype K:
Strain:
Structure:
bDGlc(1->6)aDGlc(1->4)
                    |
             ->3)bDGal(1->6)bDGalf(1->3)bDGalNAc(1->
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeQ

bDGalNAc(1->

GT NameDonorAcceptorScore / e-value
O124-WfeQ


674 / 0.0
O12-O012_O2


118 / 4e-32
O171-O171_11


90 / 2e-22
O165-WcnW


89 / 3e-22
O124-WfeS


88 / 2e-21
O164-WfeS


88 / 2e-21
O128-WbsK

bDGalNAc(1->

->4)aDGal

87 / 1e-21
O46-O046_08


86 / 3e-21
O134-O134_08


86 / 4e-21
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

85 / 7e-21
O112-WfeM


81 / 2e-19
O27-WejU


81 / 1e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeR

GT NameDonorAcceptorScore / e-value
O124-WfeR


540 / 0.0
O23-O023_05


111 / 6e-31
O82-O082_07


106 / 6e-29
O38-O038_07


91 / 1e-23
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeS

GT NameDonorAcceptorScore / e-value
O124-WfeS


692 / 0.0
O171-O171_11


128 / 1e-35
O160-O160_04


113 / 3e-30
O112-WfeM


102 / 2e-26
O185-O185_09


99 / 3e-25
O28-O028ab_05


90 / 2e-22
O143-WfdV


87 / 2e-21
O117-WbeA


84 / 2e-20
O12-O012_O2


83 / 7e-20
O107-WclW


82 / 1e-19
O112-WfeF


81 / 8e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfeT

bDGalf(1->

->3)bDGalNAc

GT NameDonorAcceptorScore / e-value
O124-WfeT

bDGalf(1->

->3)bDGalNAc

622 / 0.0
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

325 / 8e-112
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

307 / 7e-105
O96-O096_08


305 / 7e-104
O170-O170_08


287 / 5e-97
O42-WbeY

bDGalf(1->

->3)aDGlcNAc

219 / 2e-70
O28-WbeY

bDGalf(1->

->3)aDGlcNAc

218 / 4e-70
O170-O170_O4


165 / 8e-50
O167-WfdJ


103 / 6e-27
O96-O096_04


93 / 1e-23
NMR
1H-NMR5.09 4.10 4.05 4.05 4.01 3.74 4.07 4.92 3.51 3.74 3.65 4.31 4.05 4.33 4.71 3.96 3.81 4.05 3.67 3.78 3.86 4.50 3.35 3.52 3.42 3.46 3.73 3.93 4.49 3.68 3.83 4.24 3.77 3.82 3.87
Solvent: D2O
Temp: 45C
pD: 5.8
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1], pD=5.8
13C-NMR110.00 82.20 78.00 84.20 70.50 72.20 100.00 72.90 73.60 69.70 71.50 68.90 104.20 52.20 79.30 70.60 75.80 62.00 103.50 74.10 76.60 70.60 76.80 61.80 104.40 71.80 81.60 76.40 76.20 60.70
Solvent: D2O
Temp: 45C
pD: 5.8
Ref: Acetone 31.00 ppm
Comment: 13C-NMR [1], pD=5.8
Enzymes
FlippaseMFLATRTLVSLSVSFYTTRIVIQQLGAADYGLFNIIYGVVTFFTFVVTAMNDSVQRYIAIGVGSQKISVI RDAVKNSMFIFVISAFILAVCLLLARGVIIHNILNIPKDSIENASVLYLVAVFSITILIIQTPLNAMVLA YEKMSFYAYMMIFEMVAKMSMALLLTLLEKDKVIVYSILLGSISFFNLLVYLCYCLICFKKSMFGGRIKF RVLKEISTFSFWNIFGNFSYMCRVQGVNIVINMFYAIAVNAAYAISITVLNAINTLTQSLITALRPQIFK SYGECDLKRYNHLVLFGSKYTFSILFLLSSPVILCADELLKIWLDIVPDYTVEFVRLVIVVAFIDSFSYS MIAGIQATGRIKTYQLVVSLIVLINLPLTFILFKAGNNVLSMFYPFIVTAIINQGLRLYFIYINAGFDYK KYFTVVIYPCLLAVCLSLVTDISIKKMLPFNSVIDVLIVCIFIFSFNTIIFYWVVVSKKEKNGY
Trembl: B5L458
PolymeraseMYFLLFFSIFYFVYVILIGTLCHNYKALLIFSLIPLTIVSGIRYNVGFDFMSYVDYFNQLKYSNDIYLDS TFKYISYFTYYIGGNEQILFLIYAVFYSVALYFLIKLVLENYITEINNFYTIGLVLSFYSFYFLLSFNQI RAVLSALILCYGLLKNKIDFSFIITITLSILFHSAAMFILPLYFVLRKINVTVLLVIFPFLVVASFFNIF SDIVRFILTLLNSRFLTYFNSEYFVPRTGMEKMYSMISMIIVLGMVVCLSKLLPKKFDLMIKFVILFVLL RAMSIDILIFARFSDFLKPMAIILIFTAVYFSSNKVRPRIILPFYLIMTLFLCIFNIIIGSNITKGNYYT YGYNICFFGNKCIDKFY
Trembl: B5L462
Literature
  • First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
  • First Author: Linnerborg, M. "Structural studies of the O-antigen polysaccharide from the enteroinvasive Escherichia coli O164 cross-reacting with Shigella dysenteriae type 3". European Journal of Biochemistry. 266(2). (1999), pp 460-466 PubMed: [10561586]