O-Antigen Description

Serogroup:O28 ac
Serotype H:
Serotype K:
Strain:
Structure:
->2)DGro(1-P-4)bDGlcNAc(1->3)bDGalf2Ac(1->3)aDGlcNAc(1->
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbeS

DGro(1-P->

->4)bDGlcNAc

1 | 3
GT NameDonorAcceptorScore / e-value
O42-WbeS

(R)Gro(1-P->

->4)bDGlcNAc

653 / 0.0
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbeX

bDGlcNAc(1->

->3)bDGalf

2 | 2
GT NameDonorAcceptorScore / e-value
O42-WbeX

bDGlcNAc(1->

->3)bDGalf

659 / 0.0
O96-O096_07


108 / 2e-28
O112-WfeF


100 / 9e-26
O160-O160_05


88 / 1e-21
O160-O160_04


88 / 1e-21
O117-WbeA


85 / 1e-20
O115-WegI


84 / 2e-20
O107-WclW


82 / 6e-20
O171-O171_11


82 / 1e-19
O185-O185_09


81 / 4e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbeY

bDGalf(1->

->3)aDGlcNAc

3 | 1
GT NameDonorAcceptorScore / e-value
O42-WbeY

bDGalf(1->

->3)aDGlcNAc

626 / 0.0
O85-WfbU

bDGalf(1->

->3)bDGlcNAc

251 / 8e-83
O167-WfdK

bDGalf(1->

->3)bDGlcNAc

233 / 5e-76
O124-WfeT

bDGalf(1->

->3)bDGalNAc

218 / 4e-70
O164-WfeT


218 / 4e-70
O96-O096_08


212 / 7e-68
O170-O170_08


210 / 5e-67
O170-O170_O4


149 / 6e-44
NMR
1H-NMR4.69 3.72 3.76 3.97 3.51 3.78 3.89 2.08 5.21 4.88 4.35 4.26 3.92 3.64 3.68 5.01 4.06 3.86 3.47 3.84 3.80 3.86 2.05 4.02 4.06 3.89 3.76 3.76
Solvent: D2O
Temp: 50C
pD: 6
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [2], pD=6
13C-NMR101.10 56.10 73.60 75.00 75.90 61.30 23.20 175.30 106.70 82.40 83.30 83.20 71.00 63.80 21.00 173.30 97.80 53.90 77.90 69.10 73.10 61.40 23.00 174.80 65.20 78.10 62.10
Solvent: D2O
Temp: 50C
pD: 6
Ref: Acetone 31.00 ppm
Comment: 13C-NMR [2], pD=6
Non-Sugar Components
- Acetate - Glycerol - Phosphate
Enzymes
FlippaseMSLILTDFGFNLSSTEYIARNCQSKDKINKHIGAVFQIKILLIFLCVIFLYFLVNDQLQQFKIEFFFYSC IAIIGQTFQSLWFFQGVEKMKRITLFLTISKFSYVILTFVIVKKPEQGRFVILAYALSQVIGALLSQYYM RKEGYKYTPANLSSMLGVLRTSALFFWSRLAVSTYTTASTLVVGAAGHVQAGYYAASEQLYKAGQNITTP VSQALYPYMTRAKNWALFFKMMSILTVVMITASLTAIWLSQWLIALIFGPEYIVSQNVFIVFMLALIVTF VSTNFGYPVFSALGKVKVANYTVIFGALTHVVILGVLFCLGEISAYNVVRAVLFTEIVILILRMFFFVKL YRREVK
Trembl: A4K7Z5
PolymeraseMKQARIKPFSYLSLSFFVLFLIFLNISAVSFDEYLTDLQYSYAYVLFAISLVAVYYAQGESYTSLGFLFN TCLFLFIGGRFIATTFGIDSESVFTLDFMTTYIPTSDEKLEIYTRVVSFQIFLNLGYLFLRKEDSLLNLN KNDNSLPGRLLFLIVIVLFLLCLVEQYSMLSKAMSEGYLARYGDQAESYDAGAAFKYTLLFVFTGLLFAT CKKKYIIINFMIYMTLATIIILIGARGAFVSTILMGVWLYGLNKKISLKKLSIIFLSLAGILLIIMNFSA RGNYYSNELIDSLLRFLYDQGNTLAVVGYVRTITDYPVFAYISTFLPGSSFFASMFHGGSDLNSYMLSFP AYANYVANPNMYFAGYGLGWSIISSVEMFSADILFIFCIFSVLIGMFLKFANEQAKRNSFWFGAVACLCA KILFTARADIKTIIPLFMYYAIMYLIISTAMRILRQRYG
Trembl: A4K7Z7
Literature
  • First Author: Fratamico PM. "Sequencing of the Escherichia coli O2, O28ac, O63 and O42 O antigen clusters and detection of pathogenic strains by multiplex PCR assays targeting virulence genes in the respective O-antigen gene clus". Not listed.
  • First Author: Rundlöf, T. "Structural studies of the enteroinvasive Escherichia coli (EIEC) O28 O-antigenic polysaccharide". Carbohydrate Research. 291. (1996), pp 127-139 PubMed: [8864227]