O-Antigen Description

Serogroup:O113
Serotype H:21
Serotype K:75
Strain:
Structure:
  ->4)aDGalNAc(1->4)aDGalA(1->3)aDGal(1->3)bDGlcNAc(1->
         |
bDGal(1->3)
Comment:- Structure [1]. Position of acetate not determined
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnA

bDGal(1->

->4)aDGal

GT NameDonorAcceptorScore / e-value
O128-WbsK

bDGalNAc(1->

->4)aDGal

134 / 2e-38
O55-WbgO


122 / 6e-34
O55-WbgO


122 / 6e-34
O125-O125_04


111 / 6e-30
O167-WfdH


108 / 1e-28
O86-WbnJ

bDGal(1->

->3)aDGalNAc

105 / 3e-28
O86-WcmC

bDGal(1->

->3)aDGalNAc

105 / 3e-28
O86-WbwJ


104 / 1e-27
O21-WclQ


97 / 3e-25
O127-WbiP


96 / 7e-25
O90-O090_13


96 / 7e-25
O48-O048_06


95 / 3e-24
O46-O046_08


94 / 5e-24
O134-O134_08


94 / 4e-24
O23-O023_06


93 / 1e-23
O107-WclW


92 / 2e-23
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

92 / 2e-23
O117-WbeA


92 / 2e-23
O102-WelM

bDGalNAc(1->

->3)aDGal

90 / 2e-22
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

89 / 1e-22
O112-WfeF


88 / 9e-22
O81-O081_11


88 / 6e-22
O27-WejU


87 / 1e-21
O5-O05_15


86 / 3e-21
O130-WffJ


84 / 1e-20
O64-O064_03


84 / 1e-20
O161-weiG

bDGlcA(1->

->3)bDGlcNAc

84 / 1e-20
O93-O093_02


83 / 2e-20
O185-O185_03


83 / 3e-20
O112-WfeI


82 / 7e-20
O66-WfaC


82 / 4e-20
O70-O070_13


82 / 6e-20
O102-WelO

bDGlc(1->

->3)bDGalNAc

82 / 4e-20
O30-O030_06


82 / 6e-20
O182-O182_10


82 / 5e-20
O146-WbwW


81 / 1e-19
O130-WffH'


81 / 2e-19
O5-O05_12


81 / 2e-19
O104-WbwC

bDGal(1->

->3)bDGalNAc

81 / 2e-19
O71-WdaK

bDGal(1->

->3)aDGalNAc

81 / 2e-19
O38-O038_09


81 / 2e-19
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnB
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnD
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbnE

aDGal(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O3-WclR


318 / 6e-108
O40-WffO


290 / 4e-97
O69-O069_12


281 / 6e-94
O110-O110_02


270 / 2e-89
O128-WbsH

aDGal(1->

->3)bDGalNAc

254 / 2e-83
O18-wekW


247 / 2e-80
O55-WbgM


229 / 3e-74
O55-WbgM

aDGal(1->

->3)bDGalNAc

229 / 3e-74
O148-WbbG

aDGlc(1->

->3)aDGlcNAc

154 / 9e-45
O19-O019_11


154 / 4e-45
O87-WfgO


144 / 2e-41
O51-O051_12


144 / 2e-41
O120-O120_15


141 / 3e-40
O22-WbhP


116 / 3e-31
O121-WbqH


104 / 3e-27
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

95 / 3e-24
O76-O076_08


91 / 2e-22
O101-O101_07


87 / 2e-21
O89-O089_07


85 / 2e-20
O133-O133_08

aDGalA(1->

->3)aDGlcNAc

84 / 4e-20
O111-WbdH

aDGal(1->

->3)bDGlcNAc

82 / 2e-19
O18-wekV

aDGlc(1->

->4)aDGal

82 / 2e-19
O89-O089_06


82 / 2e-19
NMR
1H-NMR5.39 3.94 3.94 4.24 3.91 3.74 3.74 5.27 3.95 4.12 4.45 4.88 4.96 4.22 4.03 4.44 4.39 3.79 3.64 4.96 3.86 3.83 3.68 3.44 3.92 3.74 4.46 3.56 3.65 3.95 3.67 3.78 3.78
Solvent: D2O
Temp: 313K
pD:
Ref: Acetone 2.230 ppm
Comment: 1H-NMR [1], de-O-acetylated
13C-NMR100.36 67.81 76.51 66.75 71.16 61.29 97.06 68.60 69.06 79.32 71.45 172.49 99.84 49.71 78.15 76.14 70.75 60.65 102.06 55.01 81.16 71.73 75.86 61.68 105.85 71.82 73.77 69.50 75.79 62.06
Solvent: D2O
Temp: 313K
pD:
Ref: Acetone 31.07 ppm
Comment: 13C-NMR [1], de-O-acetylated
Non-Sugar Components
- Acetate
Enzymes
FlippaseMEKNSDKLRTGVRWSAIERIITQIAQLGILLILSRILGPKAYGLIGMLTIFIAISQTLVD SGFSSALIRRNKNSQKQYSTIFWFNLIVSLLIYAVIFFMSPHISAFYREKELSDILRVLS LLVIINALSIVHRARLTIMMDFKTQTKISLIAVLGSGVVSVILAKNGWGVWALVVNNLVF ATISTFLYFWFCKWLPDFIFQLKFFKVTAAFSVKLMIASLLNTFFDNIYQLVIGKVFYPT QVGYFTQAKNLTLLPANTYSAIIQRVTYRYFSEIKNDRVRLRQKYEEVMRISAFIFFPLT ISFSIFSNNIIVFLIGKAWEEAAKYISILSWCYVLYPIHAISLNILNVFGRSDLFLKVEI IKKIIITVCLITTYKYGISAICMGMVFYSLLATYINTIHTNRIIKTNFIALIRIISPIAF ISMLSATISFVICKFFDNNIIYIFGIMISLIIYYLIYYFVDRKIVLTIWKVNK
Trembl: Q9RP63
PolymeraseMNTTSVICFIVFLLNPSFGCFLFLLTLFNHEKLSIFFISLFAGILAYNLVPYDTMDLASH YMKFSELHNMSVSDIWSDGVNIFLYLCMKCLNAAGINKEWLPFIATFIGYYILLNALRNN IPAYWCRKEKMICFIIWLLSISFLSLSNGIRSGLSSAFFIYAVICQRNYRHLSFIIYSVL ACMMHSFAIPMVILLFSMPLILKRKMLSQRFFKIAIIMSVSLSFLVDINSLIVFIFNNLS FLPYIDQIYNIYIIGDRWGGGASFDTNTFIARLITQAPFFYCILVLLISKRKDDFFILGA ILVSVSLFLSQFWVVSERYQYVSVLLVTLHLIFNNEYFLLRRKIIILLYFVIQTAVLIWG LYRFRYMILPSLEFIIYPFLFCFYNTVDMNKLIKV
Trembl: Q9RP61
Literature
  • First Author: Parolis, H. "The structure of the O-specific polysaccharide from Escherichia coli O113 lipopolysaccharide". Carbohydrate Research. 267(2). (1995), pp 263-269 PubMed: [7534644]
  • First Author: Paton, AW. "Molecular characterization of the locus encoding biosynthesis of the lipopolysaccharide O antigen of Escherichia coli serotype O113". Infection and Immunity. 67(11). (1999), pp 5930-7 PubMed: [10531250]