O-Antigen Description

Serogroup:	O152
Serotype H:
Serotype K:
Strain:
Structure:	bLRha(1->4) \| ->3)aDGlcNAc(1->P-6)aDGlc(1->2)bDGlc(1->3)bDGlcNAc(1->
Comment:	- Structure [3]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfgB

bLRha(1->

GT Name	Donor	Acceptor	Score / e-value
O115-WegG	bLRha(1->	->4)bDGlcNAc	290 / 7e-97
O105-WbsX			147 / 3e-42

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfgC

aDGlc(1->

GT Name	Donor	Acceptor	Score / e-value
O173-WcmV	aDGlc(1->	P->6)aDGlc	328 / 5e-112
O160-O160_07			191 / 4e-59
O181-O181_03			158 / 1e-46

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfgD

bDGlc(1->

->3)bDGlcNAc

3 | 1

GT Name	Donor	Acceptor	Score / e-value
O173-WcmW	bDGlc(1->	->3)bDGlcNAc	235 / 4e-78
O56-WfaP	bDGlc(1->	->3)bDGlcNAc	205 / 2e-66
O82-O082_09			194 / 3e-62
O165-WcnW			194 / 4e-62
O38-O038_09			183 / 5e-58
O140-O140_14			179 / 2e-56
O143-WfdV			167 / 8e-52
O112-WfeI			163 / 3e-50
O161-weiG	bDGlcA(1->	->3)bDGlcNAc	159 / 1e-48
O81-O081_11			159 / 1e-48
O30-O030_06			157 / 6e-48
O134-O134_08			155 / 4e-47
O93-O093_02			154 / 1e-46
O46-O046_08			154 / 6e-47
O130-WffJ			146 / 8e-44
O21-WclQ			144 / 7e-43
O182-O182_10			144 / 3e-43
O79-WbdX			143 / 2e-42
O27-WejU			142 / 2e-42
O102-WelO	bDGlc(1->	->3)bDGalNAc	141 / 5e-42
O157-WbdN			135 / 1e-39
O24-WfaM	bDGlc(1->	->3)bDGalNAc	134 / 3e-39
O91-WbsA			132 / 2e-38
O61-WeiL			125 / 4e-36
O55-WbgP			97 / 2e-25
O55-WbgP			97 / 1e-25
O128-WbsK	bDGalNAc(1->	->4)aDGal	94 / 3e-24
O5-O05_12			90 / 1e-22
O113-WbnA			89 / 1e-22
O46-O046_05			86 / 3e-21
O134-O134_05			86 / 3e-21
O115-WegI			83 / 2e-20

ncbi CAZy

- Reference [2][3]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfgE

aDGlc(1->

->2)bDGlc

GT Name	Donor	Acceptor	Score / e-value
O24-WfaO	aDGlc(1->	->2)bDGlc	561 / 0.0
O173-WcmX	aDGlc(1->	->2)bDGlc	325 / 1e-110
O56-WfaQ			275 / 2e-91
O29-WffY			173 / 4e-52
O18-wcxD			162 / 2e-48
O81-O081_10			154 / 7e-45
O130-WffI			149 / 3e-43
O144-O144_03			148 / 9e-43
O66-WfaB			143 / 5e-41
O102-WelN	aDGal(1->	->6)bDGlc	135 / 6e-38
O85-WfbQ			133 / 4e-37
O27-WejX			120 / 1e-32
O183-wbaT			116 / 2e-31
O169-O169_04			114 / 9e-31
O187-O187_10			110 / 3e-29
O65-O065_10			97 / 1e-24
O34-O034_11			92 / 4e-23
O110-O110_03			91 / 8e-23
O178-O178_05			83 / 6e-20
O153-O153_02			83 / 6e-20
O101-O101_06			81 / 5e-19

ncbi CAZy

- Reference [2]

NMR
1H-NMR		5.37 4.07 4.22 3.81 3.95 3.84 3.94 5.27 3.57 3.75 3.51 4.12 4.09 4.91 4.23 3.64 3.38 3.44 1.33 4.75 3.64 4.11 3.58 3.47 3.78 3.98 4.71 3.45 3.55 3.43 3.43 3.71 3.92
Solvent:	D2O
Temp:	50C
pD:
Ref:	TSP 0.00 ppm
Comment:	1H-NMR [1]
13C-NMR		95.00 54.20 75.50 75.70 72.40 61.20 98.90 72.40 73.50 69.90 71.60 65.20 102.20 71.10 73.50 73.00 73.00 17.70 101.00 56.90 79.50 69.30 76.20 61.90 101.60 78.70 75.70 70.70 76.80 61.60
Solvent:	D2O
Temp:	50C
pD:
Ref:	Dioxane 67.4 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- Phosphorous

Enzymes
Flippase		MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAIALVGYAGIFDVGLTRSVIREIAIHRD NHHERTKVISTSTSFLVLFSCFGAFLLLIFSDGIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSA ILEGDEKFGIVNIQKSISSSCIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFC YKTFKRLFFFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVFPRLS NIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQISSFNILNVLLIGFFFNALAMIP FSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAISWSIRVILDALLLQWLYTRMCSVYEN
Trembl:	B5L3E9
Polymerase		MLFTYASYLCILVFSTSVAFCYEHSKSNFKVLLWSVLFLSLWIPAAIRFGVGADYFRYLSMVESVRLGYI STEVGFYFINYFVVWFDLNPQWSIAISSFIVIFLSLKAIPRNYFSISVFVLVCTFYLPSFSLVRQAIAIA FIAYAVKNYIDGNKKNYIIFILIGSLFHLSALLLVPIYFIARFRVRSSILFLGILIISLLLFLGSGLNAL LNSEFFLQSKYGYYATSDFIQDAKIGSGVGVFIKMLLPLIFITNAKKILKTNQNLNILLWMSVGCVLANV CSMKIHIFNRLADIFLFVNFMVTSYIINSLEKNVIKQLSSMAIIIVFIVFYCRTIQANYNDDLGGIGISP YKTILSK
Trembl:	B5L3F1

Literature
First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
First Author: Brockhausen, I. "Characterization of two beta-1,3-glucosyltransferases from Escherichia coli serotypes O56 and O152". Journal of Bacteriology. 190. (2008), pp 4922-32 PubMed: [18487334]
First Author: Olsson, U. "Structural analysis of the O-antigen polysaccharide from Escherichia coli O152". Carbohydrate Research. 340(1). (2005), pp 167-171 PubMed: [15620681]

bLRha(1->

bLRha(1->

->4)bDGlcNAc

aDGlc(1->

aDGlc(1->

P->6)aDGlc

bDGlc(1->

->3)bDGlcNAc

bDGlc(1->

->3)bDGlcNAc

bDGlc(1->

->3)bDGlcNAc

bDGlcA(1->

->3)bDGlcNAc

bDGlc(1->

->3)bDGalNAc

bDGlc(1->

->3)bDGalNAc

bDGalNAc(1->

->4)aDGal

aDGlc(1->

->2)bDGlc

aDGlc(1->

->2)bDGlc

aDGlc(1->

->2)bDGlc

aDGal(1->

->6)bDGlc