O-Antigen Description

Serogroup:	O9
Serotype H:	12
Serotype K:	9
Strain:
Structure:	->3)aDMan(1->3)aDMan(1->2)aDMan(1->2)aDMan(1->2)aDMan(1->
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

MtfA

aDMan(1->

->2)aDMan

1 | 1,2,3

GT Name	Donor	Acceptor	Score / e-value
O99-WejI	aDRha(1->	->2)aDRha	386 / 6e-135
O8-ORF469			203 / 9e-64
O64-O064_19			119 / 8e-33
O9-MtfB	aDMan(1->	->3)aDMan	118 / 6e-32
O99-WejJ	aDRha(1->	->3)aDRha	100 / 5e-26
O60-O060_11			95 / 5e-24
O187-O187_12			90 / 2e-22
O88-O088_12			89 / 7e-22
O59-WclB			88 / 1e-21

ncbi CAZy

- Reference [5]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

MtfB

aDMan(1->

->3)aDMan

2 | 4

GT Name	Donor	Acceptor	Score / e-value
O64-O064_19			482 / 0.0
O99-WejJ	aDRha(1->	->3)aDRha	348 / 5e-120
O60-O060_11			346 / 2e-119
O59-WclB			120 / 8e-33
O9-MtfA	aDMan(1->	->2)aDMan	118 / 6e-32
O99-WejI	aDRha(1->	->2)aDRha	109 / 8e-29
O187-O187_12			109 / 7e-29
O88-O088_12			105 / 9e-28
O11-WdaF			94 / 2e-23
O141-WfcC			93 / 3e-23
O98-WbwV	aDGalNAcA(1->	->3)aLQuiNAc	86 / 6e-21
O36-O036_10			81 / 5e-19
O7-WbbC	aDMan(1->	->4)bDGal	80 / 8e-19
O144-O144_05			80 / 8e-19

ncbi CAZy

- Reference [5]

NMR
1H-NMR		5.34 5.27 5.27 5.14 5.06
Solvent:	D2O
Temp:	95C
pD:
Ref:	Acetone 2.23 ppm
Comment:	1H-NMR [4]
13C-NMR		102.85 102.85 101.41 101.41 101.41 79.49 79.49 79.25 78.82 78.82 74.20 74.20 74.20 74.07 74.07 70.89 70.89 70.89 70.47 70.47 67.90 67.90 67.75 66.98 66.98 61.78 61.78 61.92 61.92 61.92
Solvent:	D2O
Temp:	30C
pD:
Ref:	Acetone 31.07 ppm
Comment:	13C-NMR [4]

Literature
First Author: Parolis, LA. "Structural studies of the O-antigen polysaccharide of Escherichia coli 09a". Carbohydrate Research. 155. (1986), pp 272-276 PubMed: [3539334]
First Author: Prehm, P. "The O9 antigen of Escherichia coli. Structure of the polysaccharide chain". European Journal of Biochemistry. 67(1). (1976), pp 53-56 PubMed: [786626]
First Author: Kocharova, NA. "Structure of the O-specific polysaccharide of Citrobacter braakii O7a,3b,1c". Carbohydrate Research. 333(4). (2001), pp 335-338 PubMed: [11454340]
First Author: Katzenellenbogen, E. "Structural and serological studies on Hafnia alvei O-specific polysaccharide of alpha-D-mannan type isolated from the lipopolysaccharide of strain PCM 1223". FEMS Immunol. Med. Microbiol.. 30(3). (2001), pp 223-227 PubMed: [11335142]
First Author: Kido, N. "Expression of the O9 polysaccharide of Escherichia coli: sequencing of the E. coli O9 rfb gene cluster, characterization of mannosyl transferases, and evidence for an ATP-binding cassette transport sy". Journal of Bacteriology. 177(8). (1995), pp 2178-87 PubMed: [7536735]