O-Antigen Description

Serogroup:	O61
Serotype H:
Serotype K:
Strain:	G1071
Structure:	->4)a8eLeg5Ac7Ac8Ac(2->4)bDGlc(1->3)aDGalNAc(1->
Comment:	- Structure [1] - a8eLeg5Ac7Ac is 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-Galacto-non-ulsonic acid - The O-acetylation in position 8 is 70%

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WeiK

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WeiL

bDGlc(1->

GT Name	Donor	Acceptor	Score / e-value
O130-WffJ			234 / 5e-78
O21-WclQ			234 / 3e-78
O157-WbdN			224 / 4e-74
O46-O046_08			221 / 4e-73
O134-O134_08			221 / 6e-73
O27-WejU			219 / 3e-72
O81-O081_11			216 / 1e-70
O24-WfaM	bDGlc(1->	->3)bDGalNAc	215 / 1e-70
O102-WelO	bDGlc(1->	->3)bDGalNAc	202 / 7e-66
O143-WfdV			174 / 2e-54
O112-WfeI			173 / 2e-54
O140-O140_14			169 / 7e-53
O38-O038_09			150 / 2e-45
O173-WcmW	bDGlc(1->	->3)bDGlcNAc	139 / 4e-41
O165-WcnW			138 / 4e-41
O152-WfgD	bDGlc(1->	->3)bDGlcNAc	125 / 4e-36
O182-O182_10			119 / 1e-33
O93-O093_02			117 / 6e-33
O82-O082_09			115 / 2e-32
O91-WbsA			114 / 5e-32
O30-O030_06			111 / 7e-31
O161-weiG	bDGlcA(1->	->3)bDGlcNAc	108 / 1e-29
O79-WbdX			104 / 2e-28
O128-WbsK	bDGalNAc(1->	->4)aDGal	102 / 2e-27
O56-WfaP	bDGlc(1->	->3)bDGlcNAc	96 / 4e-25
O55-WbgP			85 / 2e-21
O55-WbgP			85 / 2e-21
O125-O125_04			85 / 2e-21
O34-O034_10			84 / 5e-21

ncbi CAZy

- Reference [1]

NMR
1H-NMR		4.45 3.27 3.51 3.98 3.41 3.76 3.84 5.01 3.34 3.70 4.17 3.72 3.69 3.71 1.63 3.72 3.72 3.86 3.91 3.98 1.22 2.78
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [1]
13C-NMR		105.80 74.30 76.20 73.20 76.20 61.30 94.60 49.40 79.20 70.00 73.20 62.20 101.90 37.60 72.40 51.70 75.20 55.00 69.50 20.20
Solvent:	D2O
Temp:	40C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- Acetate

Enzymes
Flippase		MSEYKRIFQYLAFDLTNKVLPFVVPYFIASYLSSVELGYYTTKQLLFLLFFNIISMGGGA KLLVSISKKDGEEKKISSILLKMFCFNACVILFFYVLLPFFIVSENVIKDYIPLVICSLF YSIIQLQLSIYRGYNRINAYGMLNLSLSVCVCIVIFSYILYFKTQLGLWYWLIIPYALFS IKFLKYYLTERVLSSAVLLDTLKFCFYQFPHVLSSWCRLGIDRLFLANIFAMSLVGYYSM MLQFGLIVSAVLQSLNNYYSPYLFRVLSERQSYKKLSLFSKNNKAARSSFLFFVASFVIV IVVNIFAYVVVHYFLPSEYSPYYYLVPLVTFAYGLQGCYFAVVNYIYFWGKTQYLNIPSI LSCLFQVVIGYFFILHFSLLGASLSLLMSWSLQLLFTLGGVMYVAKNK
Trembl:	D8WNB8
Polymerase		MIEFLLFILCSFILYQVFYVTKEFKSNLFLIMWGYTFLFVTPVIYIFYGGEKYRVFSDES ALTFYLLGCLSAAFIILMLLFKVSLNRIKICKINLFISDFILKIIFSFCIMFVVLYILFY WREWPFFDFVSGDISDRPDIVKGTFQGFFIYSLFTSIIIPGIYFHLKDKKGKLFNLLFFI FVCFSMVVSGNKGVFLYFIIFNVLFEWKKIRLSTYLIIIVGLMAIYALIRLPFIGDNFSL SYLIESISERIFLTQGMAMPAVIELAKSTDVTMMNSNDLKYTLFNFVYGYSPGSMPLFYT AELYVRYGWLMMSFISVIISLVFGFGAFVINKTKDSAIRWVYYISLYALIMGGVGSANLF FFIVAILWWLLLTLSNGTITSRSGK
Trembl:	D8WNC0

Literature
First Author: Perepelov AV. "Structure of the O-polysaccharide of Escherechia Coli O61, Another E. Coli O-Antigen That Contains 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-D-galacto-non-2-ulosonic (Di-N-acetyl-8-epilegionaminic)". Journal of Carbohydrate Chemistry. 28. (2009), pp 463-472