O-Antigen Description

Serogroup:O109
Serotype H:
Serotype K:
Strain:G1259
Structure:
bLRhaNAc3NAc4Ac(1->3)
                   |
            ->4)aDGlc(1->3)aLTal6d2Ac(1->3)bDGlcNAc(1->
Comment:- Structure [1]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
weiM

aDGlc(1->

->3)aL6dTal

1 | 2
GT NameDonorAcceptorScore / e-value
O16-WbbK

aDGlc(1->

->3)aLRha

256 / 6e-84
O25-WekC


115 / 5e-31
O172-Orf5


105 / 3e-27
O123-WfbF


100 / 6e-26
O12-O012_05


97 / 2e-24
O186-O186_12


96 / 2e-24
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
weiN

bLRhaNAc3NAc(1->

->3)aDGlc

2 | 3
GT NameDonorAcceptorScore / e-value
O3-WclS


169 / 2e-50
O182-O182_07


158 / 1e-46
O119-WekJ

aLRhaNAc3Fo(1->

->3)bDMan

114 / 1e-30
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
weiT

aL6dTal(1->

->3)bDGlcNAc

3 | 1
GT NameDonorAcceptorScore / e-value
O66-WfaE


194 / 3e-60
ncbi CAZy
- Reference [1]
NMR
1H-NMR4.51 3.83 3.61 3.54 3.40 3.81 3.88 2.03 4.96 5.12 4.15 3.86 4.26 1.22 2.13 5.07 3.55 4.01 3.82 3.71 3.65 3.80 5.21 4.65 4.26 4.70 3.75 1.23 2.08 1.91 2.09
Solvent: D2O
Temp: 40C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [1]
13C-NMR101.40 57.50 83.40 70.30 77.80 62.20 23.70 176.00 101.00 68.20 70.90 71.40 68.70 16.80 21.90 174.40 96.80 71.00 79.90 76.50 72.90 61.30 100.70 53.00 53.40 72.90 73.10 18.20 23.50 176.70 23.20 175.50 21.60 174.80
Solvent: D2O
Temp: 40C
pD:
Ref: Acetone 31.45 ppm
Comment: 13C-NMR [1]
Non-Sugar Components
- Acetate
Enzymes
FlippaseMINEKLRKLFGLGLLFSSKLGGLVVALFLVPIYNHYMSNEAFGFVTLFLTLQSLVLLLDC GVTIILSRHTALNVCKKNKISNNQWYNTEVFLTILYLLFGFVFFIFKWLGVNWLSLISYT DLLMIIVALWATVLQNICLTVLLAGKYFSLSSVLLISGNLLKAGGAALIVKYFSPTIGAY VATQALLGISLLFVTRFFCDYFLFFKDVKRKIDFNVIKKIIHEGRALVIFNISAAVLLNL DKIIVSHNISLIALAPYFLASSLCITPISALSGPVMQFFQPGLTKAIFNDSPNVIKTLRV YISTLCVSTLLPSYILWVYRDSLIALWLGNTELVNIVADYTKILLPGIAIGGLGYISYVV LIAIEDFKFQSFLSLSMTAIVVILVVFFSMRQDIRSICYVYAFYHSLSTLLTWARCYYLP RTQKYALETAKMLFIIMIALAVLCVFFNLIINRI
Trembl: E5FGD7
PolymeraseMGCTTNQNLIYKSRVSVGSVIVLLVFCIYLFMPPMIMVLLSQSSLSAGMMVVATLLHISF AHYKILNYKILTAVLLILIYGTVSTFYSPDMKPLFSAIALIYIYTATVKFLKYLQEIHDL DAKKAISWLYYIMIIIGIMGVVFNIRFGNYAVKNYPVFPFAEPSHFALAYAPIAICYQLY YCSNKFHSFRTIGISLLLGVLYPNLILVIIGCLQFLILPKRYSVPLLFILIASVAMYIIK AGMQAIDYFYYRIFFDYSNLNLSNLVYLQGWEAAWIALKETRLFGLGFQLNGTQSAGFFT DLINSINKSELNRLDGGFLASKLISETGIIGIFFVLFLIFKAVKIIFINKKYQFSFYNKL YFSIMVSLLVELIFRGYGYFSPTFILALSFLSIPNTVCRNEITTHE
Trembl: E5FGD1
Literature
  • First Author: Perepelov AV. "Structure and gene cluster of the O-antigen of Escherichia coli O109; chemical and genetic evidences of the presence of L-RhaN3N derivatives in the O-antigens of E. coli O109 and O119". FEMS Immunol. Med. Microbiol.. 61(1). (2011), pp 47-53 PubMed: [20964722]