O-Antigen Description

Serogroup:	O158 ac
Serotype H:
Serotype K:
Strain:	E1020-72
Structure:	aLRha(1->3) \| ->4)bDManNAc(1->4)aDGalA(1->3)bDGlcNAc(1-> \| bDGlc(1->6)
Comment:	- Structure [1] - Rhamnose is O-Acetylated in position 3,4 - Reported chemical shifts are for deacetylated structure - Tentatively named O158ac here as structure differs from the previously reported one

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

wcmQ

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

wcmR

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

wcmS

GT Name	Donor	Acceptor	Score / e-value
O25-WekB			251 / 1e-81

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

wcmT

aDGalA(1->

->3)bDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O116-O116_11			366 / 3e-127
O133-O133_08	aDGalA(1->	->3)aDGlcNAc	362 / 1e-125
O100-O100_11			255 / 7e-84
O10-O010_12			254 / 2e-83
O101-O101_07			163 / 2e-48
O121-WbqI			162 / 2e-48
O89-O089_07			162 / 3e-48
O138-WfaH	aDGalNAcA(1->	->3)bDGlcNAc	154 / 4e-45
O103-WbtG			152 / 1e-44
O52-Orf16			129 / 9e-36
O111-WbdH	aDGal(1->	->3)bDGlcNAc	123 / 7e-34

ncbi CAZy

- Reference [2]

NMR
1H-NMR		4.93 4.66 3.88 3.88 3.45 3.76 4.27 1.95 5.26 3.78 3.94 4.46 4.31 4.54 3.75 3.65 3.56 3.42 3.74 3.93 2.04 4.92 3.75 3.85 3.44 4.24 1.28 4.59 3.36 3.54 3.41 3.51 3.76 3.96
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [1]
13C-NMR		98.10 50.80 75.20 71.70 75.70 68.50 23.50 175.90 102.10 69.60 70.80 78.80 72.20 174.10 101.80 56.00 83.30 72.30 77.00 62.60 23.50 176.70 100.90 71.50 71.50 73.50 70.10 18.00 103.50 74.30 77.30 71.20 77.50 62.20
Solvent:	D2O
Temp:	30C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- Acetate

Enzymes
Flippase		MSGVKKKLLKNFFSLSMLQGLNYLIPIITMPYLFRTLGPYYFGVLAIATAAINYLVVITDYGFNFIATKD ISVNRENKEKLIEIYSSVLTIKLLLCLLCFFILLLILFFSKQDCFFIQVYIFTYGIVLGQSLFSTWFFQG VEEMKYITLINVVFKLSATLFIFIIVRSKDDVLFVPIISSLGYILSGLASFFIIQRKYLISFQPQTLMTI KVYFSKNWNMFISNFFTLLYRNSNVLILGFFSSASIVASYAIAEKIIKAIQSIQMIFGTACYPYLAKKFN NDHSSFYEISKKYWLIILLAYFLLTLIVYFLSPVAIYYSTGYSDFMAIQLMKIMSFLILFGGLDYTLGVL GLIPMGYSKYFSNSVVITGIFSITLSALLSKYYGGYGASISIVLCEVLLLVALSLKIKSLYLSRGRK
Trembl:	ADN43870.1
Polymerase		MPYIVISLSPFLAAPSFLSTLGTLSFFSLIIANLYFYFKMSTSAVLPRTITPMICISFLFIICLIFSVLY HDVASKTINYFFFYVFMISCIIAISFISYTKFIDLKKINKLTLYILIFLFIFAFIEYYYIITNNGYISWV PRFERPDYASNSFSGLYRIRLFTYESSSAGALLYSLYLIFLYNIKITYQKNIFVLLISLALHLMIFSSTQ FVFFMIIIIAQFFSSNLISRKAKIFLLILVLVGIVVASHEVGILINYISNKLDGFITGNASNASASERNK LLLIGVNAFLENPIFGGGLASFYNYVDTGLVNFHLQTLQQLGIFGYLLFLTFLLYPLLFFKQYTLEIKLL FILTWFTLFFSGSIWDSNLYLPYLFIAGKSNER
Trembl:	ADN43871.1

Literature
First Author: Perepelov AV. "Structure and genetics of the O-antigen of Escherichia coli O158.". Carbohydrate Research. 346(14. (2011), pp 2274-7 PubMed: [21861990]
First Author: Quan Wang. "Development of a serogroup-specific multiplex PCR assay to detect a set of Escherichia coli serogroups based on the identification of their O-antigen gene clusters". Molecular and Cellular Probes. 24. (2010), pp 286-290 PubMed: [20561581]