O-Antigen Description

Serogroup:O158 ac
Serotype H:
Serotype K:
Strain:E1020-72
Structure:
aLRha(1->3)
         |
  ->4)bDManNAc(1->4)aDGalA(1->3)bDGlcNAc(1->
         |
bDGlc(1->6)
Comment:- Structure [1]
- Rhamnose is O-Acetylated in position 3,4
- Reported chemical shifts are for deacetylated structure
- Tentatively named O158ac here as structure differs from the previously reported one
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
wcmQ
GT nameDonorAcceptorPosition | Link.NumSimilar to
wcmR
GT nameDonorAcceptorPosition | Link.NumSimilar to
wcmS

GT NameDonorAcceptorScore / e-value
O25-WekB


251 / 1e-81
GT nameDonorAcceptorPosition | Link.NumSimilar to
wcmT

aDGalA(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O116-O116_11


366 / 3e-127
O133-O133_08

aDGalA(1->

->3)aDGlcNAc

362 / 1e-125
O100-O100_11


255 / 7e-84
O10-O010_12


254 / 2e-83
O101-O101_07


163 / 2e-48
O121-WbqI


162 / 2e-48
O89-O089_07


162 / 3e-48
O138-WfaH

aDGalNAcA(1->

->3)bDGlcNAc

154 / 4e-45
O103-WbtG


152 / 1e-44
O52-Orf16


129 / 9e-36
O111-WbdH

aDGal(1->

->3)bDGlcNAc

123 / 7e-34
NMR
1H-NMR4.93 4.66 3.88 3.88 3.45 3.76 4.27 1.95 5.26 3.78 3.94 4.46 4.31 4.54 3.75 3.65 3.56 3.42 3.74 3.93 2.04 4.92 3.75 3.85 3.44 4.24 1.28 4.59 3.36 3.54 3.41 3.51 3.76 3.96
Solvent: D2O
Temp: 30C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [1]
13C-NMR98.10 50.80 75.20 71.70 75.70 68.50 23.50 175.90 102.10 69.60 70.80 78.80 72.20 174.10 101.80 56.00 83.30 72.30 77.00 62.60 23.50 176.70 100.90 71.50 71.50 73.50 70.10 18.00 103.50 74.30 77.30 71.20 77.50 62.20
Solvent: D2O
Temp: 30C
pD:
Ref: Acetone 31.45 ppm
Comment: 13C-NMR [1]
Non-Sugar Components
- Acetate
Enzymes
FlippaseMSGVKKKLLKNFFSLSMLQGLNYLIPIITMPYLFRTLGPYYFGVLAIATAAINYLVVITDYGFNFIATKD ISVNRENKEKLIEIYSSVLTIKLLLCLLCFFILLLILFFSKQDCFFIQVYIFTYGIVLGQSLFSTWFFQG VEEMKYITLINVVFKLSATLFIFIIVRSKDDVLFVPIISSLGYILSGLASFFIIQRKYLISFQPQTLMTI KVYFSKNWNMFISNFFTLLYRNSNVLILGFFSSASIVASYAIAEKIIKAIQSIQMIFGTACYPYLAKKFN NDHSSFYEISKKYWLIILLAYFLLTLIVYFLSPVAIYYSTGYSDFMAIQLMKIMSFLILFGGLDYTLGVL GLIPMGYSKYFSNSVVITGIFSITLSALLSKYYGGYGASISIVLCEVLLLVALSLKIKSLYLSRGRK
Trembl: ADN43870.1
PolymeraseMPYIVISLSPFLAAPSFLSTLGTLSFFSLIIANLYFYFKMSTSAVLPRTITPMICISFLFIICLIFSVLY HDVASKTINYFFFYVFMISCIIAISFISYTKFIDLKKINKLTLYILIFLFIFAFIEYYYIITNNGYISWV PRFERPDYASNSFSGLYRIRLFTYESSSAGALLYSLYLIFLYNIKITYQKNIFVLLISLALHLMIFSSTQ FVFFMIIIIAQFFSSNLISRKAKIFLLILVLVGIVVASHEVGILINYISNKLDGFITGNASNASASERNK LLLIGVNAFLENPIFGGGLASFYNYVDTGLVNFHLQTLQQLGIFGYLLFLTFLLYPLLFFKQYTLEIKLL FILTWFTLFFSGSIWDSNLYLPYLFIAGKSNER
Trembl: ADN43871.1
Literature
  • First Author: Perepelov AV. "Structure and genetics of the O-antigen of Escherichia coli O158.". Carbohydrate Research. 346(14. (2011), pp 2274-7 PubMed: [21861990]
  • First Author: Quan Wang. "Development of a serogroup-specific multiplex PCR assay to detect a set of Escherichia coli serogroups based on the identification of their O-antigen gene clusters". Molecular and Cellular Probes. 24. (2010), pp 286-290 PubMed: [20561581]