O-Antigen Description

Serogroup:O165
Serotype H:
Serotype K:
Strain:G1698
Structure:
->8)aPse5NBuR3OH(2->6)bDGal(1->4)bDGlc(1->3)aDGlcNAc(1->
Comment:- Structure [2]
- Pse=5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-b-L-manno-nonulosonic acid
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcnU
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcnV

GT NameDonorAcceptorScore / e-value
O82-O082_08


364 / 2e-127
GT nameDonorAcceptorPosition | Link.NumSimilar to
WcnW

bDGlc(1->

GT NameDonorAcceptorScore / e-value
O82-O082_09


277 / 8e-95
O173-WcmW

bDGlc(1->

->3)bDGlcNAc

209 / 5e-68
O152-WfgD

bDGlc(1->

->3)bDGlcNAc

194 / 4e-62
O56-WfaP

bDGlc(1->

->3)bDGlcNAc

164 / 8e-51
O140-O140_14


163 / 2e-50
O161-weiG

bDGlcA(1->

->3)bDGlcNAc

162 / 4e-50
O143-WfdV


160 / 4e-49
O93-O093_02


157 / 6e-48
O38-O038_09


155 / 1e-47
O134-O134_08


150 / 2e-45
O112-WfeI


147 / 3e-44
O91-WbsA


145 / 2e-43
O182-O182_10


145 / 2e-43
O30-O030_06


144 / 7e-43
O46-O046_08


144 / 3e-43
O102-WelO

bDGlc(1->

->3)bDGalNAc

143 / 7e-43
O21-WclQ


142 / 2e-42
O79-WbdX


138 / 1e-40
O61-WeiL


138 / 4e-41
O130-WffJ


130 / 1e-37
O27-WejU


130 / 8e-38
O81-O081_11


127 / 2e-36
O157-WbdN


124 / 2e-35
O24-WfaM

bDGlc(1->

->3)bDGalNAc

115 / 3e-32
O55-WbgP


103 / 6e-28
O55-WbgP


102 / 1e-27
O128-WbsK

bDGalNAc(1->

->4)aDGal

102 / 2e-27
O102-WelM

bDGalNAc(1->

->3)aDGal

96 / 7e-25
O125-O125_04


95 / 1e-24
O130-WffH'


94 / 2e-24
O12-O012_O2


92 / 2e-23
O46-O046_05


90 / 9e-23
O134-O134_05


90 / 9e-23
O169-O169_03


89 / 3e-22
O183-wbaS


88 / 3e-22
O4-WbuG

aLRha(1->

->6)aDGlc

84 / 5e-21
O66-WfaC


83 / 1e-20
O18-wekT


83 / 2e-20
O185-O185_07


82 / 3e-20
O55-WbgO


81 / 8e-20
O55-WbgO


81 / 8e-20
O32-WfeA


80 / 2e-19
O27-WejW


80 / 1e-19
NMR
1H-NMR4.98 4.05 3.88 3.58 4.19 3.81 3.85 1.99 4.55 3.33 3.63 3.64 3.66 3.82 3.96 4.43 3.56 3.64 3.78 3.80 3.49 3.61 1.62 2.15 4.19 4.29 3.91 4.33 3.91 1.12 2.00 2.40 2.45 4.19 1.23
Solvent: D2O
Temp: 60C
pD:
Ref: TSP 0 ppm
Comment: - Reference [2]
13C-NMR53.6 81.8 69.8 72.7 61.8 175.3 23.4 73.9 75.9 80.4 75.6 61.4 72.0 73.7 71.1 74.4 64.2 36.0 66.1 49.5 70.2 54.3 73.1 13.2 174.4 23.3 175.7 46.0 66.2 23.2
Solvent: D2O
Temp: 60C
pD:
Ref: TSP -1.6 ppm
Comment: - Reference [2]
Non-Sugar Components
- Acetate - (R)-3-hydroxybutyryl
Enzymes
FlippaseMSFQNIVDFSFRALTLGGRFVLLIAIAKYLSPDDVGIYGILCSLISYVLYFLGIDFYVYSTRKIICGGVN KIWGMLYNQYVFYLFGYVLLIIVSQQVFTYSGISDYAVMGVAIALFEHMSQEMYRVLIVIKKIRAANIQL FIRSGSWCYITSLLLCIKNITDLKSVMFIWMLFSFISVCYALFVIYVEVHPKREDFILDLSWIIEGVKVS SFFFIGTICLRGIFYFDKIFLKDIVNLNIVGVYVFYFGVASAIQSFVDILVVSKYYPELIRQVNEDIDRG NVSLSKKIVREFLNKILATAFFLCIVSIPACYFVVKFLNKSDYLAYFPVYISLLVANVVFLIAMPYHYFL YALKRDSFLVRINIFTFGLFLLLSYVLIRLFSDLTVYCIIVPIIVSYSSALFIKFYGYNSFVRGRS
Trembl: E2DNQ3
PolymeraseMNKNSNFFLFLLVIDVFFTIILCCASYYFHENYTLRCITLFLFIALLLIILKINFLFFYLSLLLIYGYSG QILSLFFIEQGGSYLNELQNQSHSNGAMSLLILIVTFAIMINYIFFTLVFRNLTKVRLWFANGNNTVRLI NVLFIFAYISIIFLYGNAYGSAGGNRVIYRHDYLPSWAGIFIDLQWIPVLVLGLLYINNKKKEFFIFLIL HLFFRVLFGEKFTMLVQLLYISFIPYVIFFHQEINVNRFIKIVLFFGVLLGTSIFLNYFFIENAGFERIE QRIAQQGQLWWYVVNNYLEKGGSISVINEFFSYNKGDCQQGMDLLMCKSMSPSLYQLYIEKQYVLTSGYP SILLLYFGPYGIILFLFASPLFLLPLVMLYVSLKNKKILASIISFRVFFSFFSFYAIGNMHDLLNLKTLI YFLLWLGVYSIPKIVFVNRKACNYD
Trembl: E2DNQ5
Literature
  • First Author: Wang Q. "Development of a serogroup-specific multiplex PCR assay to detect a set of Escherichia coli serogroups based on the identification of their O-antigen gene clusters". Molecular and Cellular Probes. 24(5). (2010), pp 286-90 PubMed: [20561581]
  • First Author: Senchenkova S. "Structure and gene cluster of the O-antigen of Escherichia coli O165 containing 5-N-acetyl-7-N-[(R)-3-hydroxybutanoyl]pseudaminic acid". Glycobiology. 26. (2016), pp 335-342 PubMed: [26582605]