ECODAB

O-Antigen Description

Serogroup:	O184
Serotype H:
Serotype K:
Strain:	99-4473
Structure:	->2)aDFuc3NBuR3OH(1->3)aDMan6Ac(1->3)aLFuc(1->3)aDGalNAc(1->
Comment:	- Structure [2]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O184_08

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O184_10

GT Name	Donor	Acceptor	Score / e-value
O43-O043_08			320 / 3e-109
O11-WdaF			231 / 8e-75
O36-O036_10			225 / 2e-72
O125-O125_08			219 / 5e-70
O99-WejJ	aDRha(1->	->3)aDRha	89 / 6e-22
O144-O144_05			87 / 2e-21
O64-O064_19			81 / 1e-19

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O184_15

GT Name	Donor	Acceptor	Score / e-value
O125-O125_13			386 / 2e-137
O43-O043_13			385 / 4e-137
O11-WdaG			381 / 1e-135
O41-O041_12			265 / 6e-90
O168-WfeY			263 / 3e-89
O156-O156_14			262 / 7e-89
O84-O084_13			261 / 1e-88
O159-WfgS			260 / 4e-88
O36-O036_15			259 / 1e-87
O11-WdaD			133 / 7e-39
O43-O043_04			123 / 3e-35
O111-WbdL	aDGlc(1->	->4)aDGal	110 / 5e-30
O173-WcmU	aLFuc(->	->4)aDGlc	108 / 2e-29
O36-O036_08			103 / 1e-27
O168-WfeV			102 / 2e-27
O41-O041_06			87 / 5e-22
O157-WbdO			84 / 5e-21

ncbi CAZy

- Reference [1]

NMR
1H-NMR		5.62 3.92 4.49 3.77 4.23 1.23 5.11 4.19 4.07 3.74 3.79 3.71 3.86 4.93 3.84 4.01 3.96 4.10 1.19 5.22 4.45 3.73 4.00 3.85 3.76 3.79 2.45 2.53 4.23 1.25 2.06
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2]
13C-NMR		96.7 69.8 50.2 71.9 68.5 16.5 102.9 71.2 76.0 68.7 74.7 62.1 102.6 68.7 78.4 72.7 68.3 16.7 94.7 49.5 77.3 70.0 72.7 62.1 175.4 46.0 66.2 23.7 23.7 176.2
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP -1.6 ppm
Comment:	13C-NMR [2]

Non-Sugar Components
- Acetate - (R)-3-hydroxybutyryl

Enzymes
Flippase		MKRLLKVSALSGMLTLLKMLMGFIIAKVIAVYTGPAGMAMLGQIQNIINSFNGIVNSPVS NGVVRFTAENNKSGFYKCSPWWRASLEWLIILCVITIPLGFLFSDYLSTWLFETKEFGWL IKLTVIFLPLTAFGTLISSVINGLENFRRYIILIMVSVLISSCLMIVMIIYAGLKGALIA AVFQSSMIGAVMLLTNIKQPWFSLTYWWGNTNSKARKDIAGYILMAVTSAITAPLSLILV RNIIIYHSGWEQAGQWQAVWKISEVYLSIITISLSTYFLPKLASKADVGGIIQEINKTAL IVIPLAIIMAVVVYAGRDIAIYILFTEQFRAARELFAIQLLGDIVKIISWLYAYPMLSRG ATKWYVSTEILFSASFVILSWFLVGEYGNAHGANVAYLINYTFYFLFIIVNFKKIIK
Trembl:	A0A0A8J6L5
Polymerase		MPIAVYFIEFAYVMIVLLFILYYNSRNMPFIEGLFLILIICSPYSISIIPFRQDMYEIYG IYSNNGILNIVNFYKIAGLSILDMMALLIIVININTVMKIPSFFIMFYFLVGCFSIIGYS ISSYLFWSPELDNIFNILSVFKSIIYIISVYCAIDRICKHIGFDKFIKFLANLILCYCII NALILCFLPDWYTWVKYSFNYLFLDQTDQFLVFLYCLLAVWGPLSGIHRYKIYALILCIM LLLSGGKGGVYSLVILAMAWLYKNSNINSAILSICFFLIMILSWMLSYYIGFNVFDLSIY TRYFQVNQLFLNYMNNILTIFFGIGPSKAYFFYSEPAILDPGAYTSSELNSSFKIGFQMP YLTWIKNFGLSGLILLYLSLNYVVKTARHSKSNEAYFSGIVYALGLYFLLVGFMDFPSFG LKTIIPISLYIYIVKMNATNIHCEGRK
Trembl:	A0A0A8J752

Literature
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
First Author: Senchenkova SN. "Structure and genetics of the O-antigens of Escherichia coli O182-O187". Carbohydrate Research. 435. (2016), pp 58-67 PubMed: [27710814]