O-Antigen Description

Serogroup:O186
Serotype H:
Serotype K:
Strain:182-02
Structure:
->3)bDQui4NLAlaBuS3OH(1->6)aDGlc(1->3)aLQuiNAc(1->3)aDGlcNAc6Ac(1->
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
O186_10

GT NameDonorAcceptorScore / e-value
O123-WfbE


662 / 0.0
O116-O116_07


157 / 2e-46
O121-WbqE


97 / 5e-25
GT nameDonorAcceptorPosition | Link.NumSimilar to
O186_12

aDGlc(1->

GT NameDonorAcceptorScore / e-value
O123-WfbF


645 / 0.0
O25-WekC


263 / 9e-87
O172-Orf5


176 / 3e-53
O16-WbbK

aDGlc(1->

->3)aLRha

127 / 1e-35
O12-O012_05


124 / 4e-34
O109-weiM

aDGlc(1->

->3)aL6dTal

96 / 2e-24
GT nameDonorAcceptorPosition | Link.NumSimilar to
O186_16

aLQuiNAc(1->

->3)aDGlcNAc

GT NameDonorAcceptorScore / e-value
O123-WbwH


649 / 0.0
O163-O163_11


414 / 3e-146
O26-WbuB

aLFucNAc(1->

->3)bDGlcNAc

347 / 4e-120
O172-WbuB

aLFucNAc(1->

->3)aDGlcNAc

342 / 1e-117
O177-WbuB


341 / 3e-117
O108-O108_18


340 / 4e-117
O25-WbuB


339 / 2e-116
O151-WbfG


338 / 1e-116
O118-WbfG


338 / 1e-116
O4-WbuB

aLFucNAc(1->

->3)bDGlcNAc

338 / 4e-116
O15-WbuB


337 / 7e-116
O98-WbwW

aLQuiNAc(1->

->3)bDGlcNAc

337 / 6e-116
O181-O181_07


335 / 2e-115
O12-O012_09


335 / 1e-115
O145-WbuB

aLFucNAm(1->

->3)bDGlcNAc

333 / 4e-114
O29-WbuB


329 / 1e-112
NMR
1H-NMR4.44 3.43 3.78 3.79 3.54 1.16 4.99 3.46 3.65 3.55 4.09 3.86 4.04 4.94 4.08 3.71 3.30 4.20 1.25 4.99 4.04 3.83 3.59 4.22 3.81 4.20 1.26 2.41 4.17 1.22 1.95 2.00
Solvent: D2O
Temp: 30C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [2]
13C-NMR104.0 73.8 77.4 58.2 72.5 17.7 101.2 72.7 74.3 70.3 72.0 69.5 98.4 54.7 80.1 75.2 69.4 17.8 97.8 54.6 76.8 69.2 73.0 61.5 175.6 51.2 17.5 174.7 45.8 66.5 23.3 23.7 23.8 175.1 175.8
Solvent: D2O
Temp: 30C
pD:
Ref: TSP -1.6 ppm
Comment: 13C-NMR [2]
Non-Sugar Components
- Acetate - (S)-3-hydroxybutyryl - L-Alanine
Enzymes
FlippaseMLLRRYMNSNVKKNISAVNGLKWSAIERICSQGIQLLLMIVLARQLGPGAFGLIGMLTIF ITIGQVFIDSGFSAALIRKNERTESDYATVFYFNMTVAILFYAVLFFCAPFIAEFYKRNE LIELTRVLGLTIIISAFIIVQRIQLSVILDFKTQAISSLSSVIISGGCALLMAYNGFGVW SLVIQTITMGLVNLVILNIYNPWLPKRSFSKKSFHGFFSFGSRLLISSLIDSIYTNIYLV VIGKSFSASTLGQFTQANLLSNTPAMTLTTVVQRVTYPLLSNVNNAKGNIDEIYLRILRL TAAAVFPVMFLLAIIAKPFVVLFLGQQWEPVAELMSILCIGYCLYPVHAINLNLLQVKGR TDLFLKLEIIKKTLITVILIVTIPFGVKIICIGIFAQYYISLLINTYYTGKLSSLSAIAQ IKALLPIWLMASISSAISWFLIPREIFSGLYQIIGILITNISLYGIGMYLFQKDIYEMVK FLFI
Trembl: A0A0A8J772
PolymeraseMYLTTNYCDFLSRTRIVRRIFLLVYLISNVYAYIVFTETGVLIGDYQGVLIIYKDQVVYL LMLTIMSYYIVCGPVFNCLSKVKIRLRTFKGLNSFAYILFFFQILFAVFNISTGSNAAGT DFQTGGVIRLLWLFLPVDYLFYIYYFVGREKKVSKIYLANVVIFILSMLSRGWLGWTLVL LYAELCFFFYSQKKIKIKYLILLFFLLIVAPLAFSLKIQLRADLYSSGIGGVISTLSNID YIQSYNNFIAGFLSRIQQLSNIVFFYDHQQELYKFVSSDIVSNYAWEGLPQQTVAKLLGL DPGVDMHIFLYSHYISSTSEAVTTLQVGFISWLFLGTLSSVFYPLFVFAIICISLFLSKK LGGEKLCALTWIMIFLSIMCGWYNAYLVYMQALITFYFIMGFLNLITLEKTKINHT
Trembl: A3E2C7
Literature
  • First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
  • First Author: Senchenkova SN. "Structure and genetics of the O-antigens of Escherichia coli O182-O187". Carbohydrate Research. 435. (2016), pp 58-67 PubMed: [27710814]