O-Antigen Description

Serogroup:O174 ab
Serotype H:
Serotype K:
Strain:
Structure:
bDGlcNAc(1->2)
            |
     ->4)bDGlcA(1->3)bDGal(1->3)bDGalNAc(1->
Comment:- Structure [4]
- Name of O174ab is proposed here until decided otherwise
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbcX
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbcY

GT NameDonorAcceptorScore / e-value
O64-O064_07


244 / 2e-80
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbcZ

bDGal(1->

->3)bDGalNAc

GT NameDonorAcceptorScore / e-value
O178-O178_09


279 / 1e-94
O153-O153_06


279 / 1e-94
O146-WbwW


273 / 3e-92
O155-WclG


267 / 5e-90
O104-WbwC

bDGal(1->

->3)bDGalNAc

266 / 7e-90
O33-O033_09


263 / 3e-88
O37-O037_13


261 / 9e-88
O112-WfeJ


259 / 3e-87
O160-O160_08


258 / 2e-86
O171-O171_13


256 / 1e-85
O71-WdaK

bDGal(1->

->3)aDGalNAc

254 / 4e-85
O5-O05_15


247 / 4e-82
O185-O185_03


235 / 1e-77
O117-WbeD

bDGal(1->

->3)aDGalNAc

230 / 8e-76
O107-WclZ

bDGal(1->

->3)aDGalNAc

229 / 2e-75
O70-O070_13


228 / 1e-74
O131-O131_11


222 / 2e-72
O65-O065_13


178 / 2e-55
O7-WbbD

bDGal(1->

->3)aDGlcNAc

172 / 4e-53
O28-O028ab_02


130 / 2e-37
O63-WbuP


114 / 1e-31
O64-O064_03


110 / 7e-30
O114-WbuP

bDGal(1->

->3)aDGlcNAc

102 / 4e-27
O54-O054_06


101 / 1e-26
O74-O074_09


96 / 9e-25
O167-WfdH


92 / 1e-23
O92-O092_07


81 / 1e-19
ncbi CAZy
- Reference [2]
NMR
1H-NMR4.81 3.67 3.56 3.37 3.47 3.71 3.93 2.04 4.68 3.61 3.65 3.78 3.69 4.53 4.01 3.83 4.17 3.70 3.77 3.80 2.02 4.51 3.68 3.68 4.10 3.64 3.72
Solvent: D2O
Temp: 58C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [4]
13C-NMR102.76 56.86 74.54 71.13 77.01 61.96 23.11 175.54 103.19 81.60 75.30 80.26 77.06 175.15 101.54 51.72 81.10 68.70 75.64 61.94 23.50 175.42 104.71 70.38 84.96 68.52 75.53 61.85
Solvent: D2O
Temp: 58C
pD:
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [4]
Enzymes
FlippaseMLTKYTLIRNSILNLSGYIIPTLVAIPALGYMARELGPELFGVYTLAMALVGYASVFDFGLTRAIIREIAIYRDDIVEKR KIISTSTVFLTVIGFVVTELIFINVNTIVTFINVSKNNFEDANIALKILSLSIPLLLLNQLWVSVFEGEEKFGLINIQKT ISNTCIVALPAVSILIESSLTYAVSGLVVGRVISLILSFLFLRKEIVASGIRFHTIVMKRLIMFGSWMTFSNIISPMMVY FDRFIISNILGAKNVGFYTAPAEIISRMSIIPTSVSRALFPRLSNMSDFKKFKCELLFSYAIMISICVPIVLFLLLFSGG IMYYWLGPQYYLKSSSVFSVLLIGFFFNSLAQLPFSAIQSLGNSKITALLHGFEIIPYLIILYILTSHFGIVGTAYAWTL RVMFDFIALYFLSSWLIKKKH
Trembl: Q3IA96
PolymeraseMLKRYSNLIFSFVIAFSFFSYVMNPSLKFIYKSPVINFIPFLLCCLITIIFLTEKKIKLNYYELFSILYV GFFFILQYTYFIQSTDSIETLLRLMSVNFSFLFGLLLGWCFKRESIEKLFILWVLLLSIANICGVINYSD GVEFNHLNFTLPLGTVVTWLIFKAFMKDTDRYIFTLSVILFLFFNIIFAGSRTAIFLPILVSLFIMVIFR KYVSIKKTVISFIILITITIISLPYILSNLNAYFLSKVQNMADISEDSRYNLYLKCFNMLLEHPLGIGYG NYKYFITEPYPHNILLEIGLNSGVLGCFVFITYVMITTIVIIKKTKSCYNEKNLFVLTIFLYSLFSWMFS NDFASSSVVFFLLGVLGHIASSNNEKEIK
Trembl: Q3IA92
Literature
  • First Author: Scheutz,. "Designation of O174 and O175 to temporary O groups OX3 and OX7, and six new E. coli O groups that include Verocytotoxin-producing E. coli (VTEC): O176, O177, O178, O179, O180 and O181". Acta Pathologica, Microbiologica, et Immunologica Scandinavica. 112(9). (2004), pp 569-84 PubMed: [15601305]
  • First Author: Beutin,. "Development of PCR assays targeting the genes involved in synthesis and assembly of the new Escherichia coli O 174 and O 177 O antigens". Journal of Clinical Microbiology. 43(10). (2005), pp 5143-5149 PubMed: [16207976]
  • First Author: Drummelsmith, J. "Gene products required for surface expression of the capsular form of the group 1 K antigen in Escherichia coli (O9a:K30)". Not listed. 31(5). (1999), pp 1321-1332 PubMed: [10200954]
  • First Author: Fontana C. "Structural studies and biosynthetic aspects of the O-antigen polysaccharide from Escherichia coli O174". Carbohydrate Research. 354. (2012), pp 102-105 PubMed: [22572125]