O-Antigen Description

Serogroup:	O43
Serotype H:
Serotype K:
Strain:	Bi7455-41
Structure:	aLFuc(1->3) \| ->4)bDMan2Ac(1->3)aDMan(1->3)aLFuc(1->3)bDGalNAc(1->
Comment:	- Structure [2] - The first mannose residue is O-acetylated 70% - Strain used for structure elucidation was G1247

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O043_04

bDGalNAc(1->

GT Name	Donor	Acceptor	Score / e-value
O11-WdaD			174 / 8e-55
O43-O043_13			124 / 1e-35
O184-O184_15			123 / 3e-35
O125-O125_13			122 / 1e-34
O11-WdaG			121 / 2e-34
O173-WcmU	aLFuc(->	->4)aDGlc	119 / 9e-34
O84-O084_13			115 / 2e-32
O159-WfgS			114 / 5e-32
O36-O036_15			113 / 2e-31
O168-WfeY			112 / 3e-31
O156-O156_14			110 / 2e-30
O41-O041_12			108 / 1e-29
O168-WfeV			92 / 8e-24
O36-O036_08			88 / 2e-22
O157-WbdO			87 / 5e-22
O111-WbdL	aDGlc(1->	->4)aDGal	83 / 1e-20
O12-O012_O2			82 / 5e-20
O41-O041_06			81 / 5e-20
O128-WbsK	bDGalNAc(1->	->4)aDGal	80 / 2e-19

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O043_06

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O043_08

GT Name	Donor	Acceptor	Score / e-value
O184-O184_10			320 / 3e-109
O11-WdaF			218 / 2e-69
O36-O036_10			201 / 4e-63
O125-O125_08			194 / 1e-60
O99-WejJ	aDRha(1->	->3)aDRha	94 / 7e-24
O175-O175_05			86 / 4e-21
O141-WfcC			86 / 1e-20
O64-O064_19			81 / 4e-19
O187-O187_12			80 / 7e-19

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O043_13

GT Name	Donor	Acceptor	Score / e-value
O125-O125_13			496 / 0.0
O11-WdaG			488 / 0.0
O184-O184_15			385 / 4e-137
O41-O041_12			261 / 3e-88
O159-WfgS			260 / 6e-88
O84-O084_13			259 / 7e-88
O168-WfeY			258 / 3e-87
O36-O036_15			257 / 7e-87
O156-O156_14			257 / 8e-87
O11-WdaD			135 / 8e-40
O43-O043_04			124 / 1e-35
O173-WcmU	aLFuc(->	->4)aDGlc	122 / 2e-34
O36-O036_08			112 / 9e-31
O111-WbdL	aDGlc(1->	->4)aDGal	109 / 1e-29
O168-WfeV			108 / 2e-29
O41-O041_06			95 / 1e-24
O157-WbdO			88 / 2e-22

ncbi CAZy

- Reference [1]

NMR
1H-NMR		5.05 3.82 4.09 3.87 4.77 1.27 4.85 4.26 3.83 4.02 3.46 3.75 3.93 5.12 4.23 4.14 3.80 3.80 3.79 3.89 5.00 3.83 4.06 3.96 4.14 1.20 4.49 4.04 3.76 3.95 3.62 3.74 3.74 2.03
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2]
13C-NMR		96.0 69.4 70.8 73.3 68.0 16.5 98.3 68.5 75.7 71.9 77.0 61.5 103.2 68.5 78.7 66.5 74.4 62.3 102.6 68.8 78.2 72.7 68.3 16.4 102.0 52.9 79.6 69.0 76.1 62.6 176.2 23.4
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP -1.6 ppm
Comment:	13C-NMR [2]

Literature
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
First Author: Perepelov AV. "Structure and gene cluster of the O-antigen of Escherichia coli O43". Carbohydrate Research. 416. (2015), pp 32-36 PubMed: [26342864]