ECODAB

O-Antigen Description

Serogroup:	O156
Serotype H:
Serotype K:
Strain:	E1585-68
Structure:	->4)aLFuc(1->6)aDGal(1->3)aLFuc(1->3)bDGlcNAc(1-> \| aDGal4,6(R)Pyr(1->3)
Comment:	- Structure [2] - Strain for structure elucidation was G2315

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O156_06

GT Name	Donor	Acceptor	Score / e-value
O41-O041_04			264 / 1e-87
O84-O084_08			114 / 1e-30

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O156_08

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O156_09

GT Name	Donor	Acceptor	Score / e-value
O159-WfgR			112 / 6e-30
O151-WbhW			99 / 1e-25
O118-WbhW			99 / 1e-25

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O156_14

GT Name	Donor	Acceptor	Score / e-value
O168-WfeY			508 / 0.0
O84-O084_13			483 / 0.0
O159-WfgS			482 / 0.0
O41-O041_12			472 / 0.0
O36-O036_15			465 / 0.0
O125-O125_13			262 / 1e-88
O184-O184_15			262 / 7e-89
O11-WdaG			261 / 1e-88
O43-O043_13			257 / 8e-87
O11-WdaD			125 / 6e-36
O43-O043_04			110 / 2e-30
O173-WcmU	aLFuc(->	->4)aDGlc	108 / 1e-29
O168-WfeV			103 / 6e-28
O111-WbdL	aDGlc(1->	->4)aDGal	101 / 1e-26
O36-O036_08			95 / 1e-24
O41-O041_06			84 / 4e-21

ncbi CAZy

Reference [1]

NMR
1H-NMR		4.63 3.84 3.69 3.52 3.39 3.67 3.87 5.02 3.91 3.90 3.95 4.33 1.17 5.19 3.83 3.94 4.05 4.25 3.57 3.85 4.88 4.02 3.96 4.03 4.06 1.24 5.32 3.91 4.02 4.26 3.96 3.90 4.04 2.01 1.50 2.04
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP 0 ppm
Comment:	1H-NMR [2]
13C-NMR		102.1 57.1 81.3 70.0 77.5 62.3 100.7 68.4 79.7 72.9 68.1 16.6 101.8 69.9.0 70.5 70.5 70.7 67.6 99.8 69.3 75.4 78.9 68.3 17.1 26.3 100.9 175.7 23.5 175.7 23.6 175.5
Solvent:	D2O
Temp:	40C
pD:
Ref:	TSP -1.6 ppm
Comment:	13C-NMR [2]

Non-Sugar Components
- Pyruvic acid

Enzymes
Flippase		MQQLAELGLGQTLRLHIAHAFKMDESGWCFNSKKEIKQYYSLSVKWFLVVALCTIIIIGP AGIYYYHSYQGDIIWEGPWVSLVLVTALTIIITPIQITIESLQHQISVFKAQTYASIINS ISLCLLLFSGFGLYAIALSLFISNLTVLIFLWGKIKWLRNNLMPVLVDTNFFVVFKEIWP LFSRVAVVSVFGFLFWNGFNLIAFKVYEPSYAGQVILSITLARAGYGFSESILYGQITYI SNLISNKKYNEAMIVYKKNTYIALTILLIGYVFFLLLMYLFPEFYLFKKIVGSSYLISIF VFFIILLFLTSGNNYVRCFKTEPFVKVSIFHGVMVPTLFYLSSVFYEGYYLYPCSLVLIV SLILSKRISNKYFRMSGIN
Trembl:	A0A0A8J8V2
Polymerase		MENQHVYFYKKSNKANVSIVALIFTYISIIGWSPFFSGYLAVPLFLTYHIPFLLLLAYFM LTQKISSALISLSIIMIILISALTLLTESLINFNRYIFEPLTFLLCYYCTVSRKLAEKIC TYASCVVIIGIVLSVISFCYAYAGGNEILTFHNVDGRLNSLYLTSFSNFKIGNIIRPSFI YDEPGAFSFVICFVVILREVLGRKYNWVYLLGGMITLSLTQFIILSLYIIVKNWKNIKQI LVLCFALFGLIMFTYNNNEFDFFYNRFNTENGTFSGDNRSMQISNYLNVVNENMFLLGDY KCHDNINQRCESHGDISSSIVTPIYKGGILQLLLQIITHAIIFWLALRERKLIFPALVLT ILLLQRPFFDVVGYSVMIYYSIFLMFNFKVFKDDYYHSRKK
Trembl:	A0A0A8J703

Literature
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
First Author: Duan Z. "Structure and gene cluster of the O-antigen of Escherichia coli O156 containing a pyruvic acid acetal". Carbohydrate Research. 430. (2016), pp 24-28 PubMed: [27177202 ]