O-Antigen Description

Serogroup:	O28 ab
Serotype H:
Serotype K:
Strain:	K1a
Structure:	->3)bDGlc(1->3)DGro(1-P-4)bDGlcNAc(1->3)aDGlcNAc(1->
Comment:	- Structure [2] - Strain is given for the GT study

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O028ab_02

->3)aDGlcNAc

GT Name	Donor	Acceptor	Score / e-value
O178-O178_09			137 / 8e-40
O153-O153_06			137 / 8e-40
O5-O05_15			135 / 5e-39
O171-O171_13			135 / 2e-39
O174-WbcZ			130 / 2e-37
O117-WbeD	bDGal(1->	->3)aDGalNAc	128 / 2e-36
O107-WclZ	bDGal(1->	->3)aDGalNAc	127 / 3e-36
O104-WbwC	bDGal(1->	->3)bDGalNAc	127 / 5e-36
O65-O065_13			123 / 1e-34
O71-WdaK	bDGal(1->	->3)aDGalNAc	122 / 3e-34
O185-O185_03			120 / 2e-33
O33-O033_09			120 / 1e-33
O63-WbuP			116 / 4e-32
O112-WfeJ			116 / 3e-32
O160-O160_08			116 / 3e-32
O37-O037_13			116 / 5e-32
O146-WbwW			115 / 6e-32
O7-WbbD	bDGal(1->	->3)aDGlcNAc	112 / 2e-30
O70-O070_13			111 / 2e-30
O155-WclG			108 / 4e-29
O131-O131_11			101 / 6e-27
O54-O054_06			89 / 2e-22
O64-O064_03			88 / 5e-22
O167-WfdH			83 / 3e-20

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

O028ab_05

GT Name	Donor	Acceptor	Score / e-value
O12-O012_O2			103 / 5e-27
O160-O160_04			91 / 6e-23
O124-WfeS			90 / 2e-22
O164-WfeS			90 / 2e-22
O112-WfeI			89 / 2e-22
O117-WbeA			82 / 5e-20
O171-O171_11			81 / 3e-19

ncbi CAZy

- Reference [1]

NMR
1H-NMR		5.16 3.99 3.91 3.60 4.05 3.80 3.83 4.59 3.73 3.78 3.97 3.55 3.81 3.90 3.95 3.99 4.05 3.71 4.01 4.48 3.38 3.57 3.59 3.43 3.72 3.90
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP 0 ppm
Comment:	- Reference [2]
13C-NMR		99.2 54.2 80.3 69.5 72.7 61.5 102.5 56.9 73.8 75.5 76.2 61.8 67.8 70.7 72.1 104.2 73.3 83.6 71.4 77.1 61.8
Solvent:	D2O
Temp:	30C
pD:
Ref:	TSP -1.6 ppm
Comment:	- Reference [2]

Non-Sugar Components
- Phosphate

Enzymes
Flippase		MIGLKKNIIFMLIYQIVNYLVPLALTPYLVRILGPSSYGELNYSISIVLYICLFVDFGFT LSATKRITKYIGNSDKISKLFYTVTVAKTFLLITGITILILSISTLDTINSVKTIIVISA IQIIATVISPLWLFNGLQLTAVFAVFNIIFKVASIPLVFFLVKTPTDVGYAAFFQTAPLF FSSILALIYLYKKKILIYTNYTPRFKLIIRTLNSSLFYYIGTMSVSLYTMSTPIILGLVS TSEQVGFYSASDKIRAAVVGVYVVIGNVIYPRVQRFYHEDDNLGFQYVKKIIIAVIPLCT IASIMLFYISPFITKYFLGEEFGESELILKIMSPMMFLIPLSIILSNYILLGMGHKKIFS RIPMITATIHIIVATILSLKFGSIGASIAILISEIVSFILLFITCCKLGYIKKLCVLKI
Trembl:	A0A0B0V6F4
Polymerase		MSIGILVLVFYVIVNFVMGIVFFNDGFLGGDFYGEKIDNKDLFLWVLGGELLLLITFYAI AVLIVKFTFNKRYPEDYYTVSELALKRFNIFLIVLQTSFILFIFATGFGRISLLMDENSS AANINSPIKYIFVILLPDFLFIVYFLVQKNNKKTIVNLSLYLISNIVRGWVSGPVLLIMA IFLVKKFSFKKIKIKTIISLVTCFIIFVPLVTSLKFGVRFQNDEISAVDLVQDSIENNVS YYLRTLNRFQHISEAYSFMDSMTFIRKDYADNRIALPFFTNDFINITSKIFSFEPQNLLT YGSKVLLDRPFGNIQSGILPWLSIDYFFSISYILTFFIFTILLSRFCKMLTLGYPVSKNI VIWFTIYYYAHGWLFSYMNLLIAMMIFIIFSKVFGGKILNKDNNYA
Trembl:	A0A0A8J443

Literature
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]
First Author: Shashkov A. "Structures and genetics of biosynthesis of glycerol 1-phosphate-containing O-polysaccharides of Escherichia coli O28ab, O37 and O100". Carbohydrate Research. (2016), PubMed: [27058293]