O-Antigen Description

Serogroup:O123
Serotype H:
Serotype K:
Strain:
Structure:
->3)bDQui4NLAlaBuS3OH(1->6)aDGlcNAc(1->3)aLQuiNAc(1->3)aDGlcNAc(1->
Comment:- Structure [3]
- Another structure has been proposed [1]
- The first aDGlcNAc is O-acetylated in position 6, 30%
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfbE

GT NameDonorAcceptorScore / e-value
O186-O186_10


662 / 0.0
O116-O116_07


157 / 3e-46
O121-WbqE


98 / 3e-25
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WfbF

GT NameDonorAcceptorScore / e-value
O186-O186_12


645 / 0.0
O25-WekC


278 / 1e-92
O172-Orf5


192 / 3e-59
O12-O012_05


128 / 2e-35
O16-WbbK

aDGlc(1->

->3)aLRha

127 / 5e-35
O109-weiM

aDGlc(1->

->3)aL6dTal

100 / 6e-26
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbwH

aLQuiNAc(1->

->3)aDGlcNAc

GT NameDonorAcceptorScore / e-value
O186-O186_16


649 / 0.0
O163-O163_11


422 / 2e-149
O172-WbuB

aLFucNAc(1->

->3)aDGlcNAc

383 / 2e-133
O177-WbuB


382 / 5e-133
O25-WbuB


378 / 2e-131
O4-WbuB

aLFucNAc(1->

->3)bDGlcNAc

378 / 2e-131
O15-WbuB


375 / 3e-130
O145-WbuB

aLFucNAm(1->

->3)bDGlcNAc

370 / 2e-128
O29-WbuB


366 / 7e-127
O151-WbfG


352 / 2e-121
O118-WbfG


352 / 2e-121
O26-WbuB

aLFucNAc(1->

->3)bDGlcNAc

350 / 8e-121
O108-O108_18


347 / 1e-119
O181-O181_07


345 / 6e-119
O12-O012_09


343 / 3e-118
O98-WbwW

aLQuiNAc(1->

->3)bDGlcNAc

330 / 7e-113
O177-WbdZ


86 / 1e-20
ncbi CAZy
- Reference [2]
NMR
1H-NMR5.03 3.91 3.71 4.24 3.70 4.08 3.90 4.99 4.04 3.87 3.59 4.25 3.82 3.80 4.95 4.07 3.79 3.28 4.26 1.25 4.46 3.46 3.79 3.80 3.55 1.18 2.42 4.18 1.23 4.22 1.26
Solvent: D2O
Temp:
pD:
Ref: Acetone 2.23 ppm
Comment: 1H-NMR [1]
13C-NMR97.98 54.37 71.82 71.28 69.89 68.52 97.29 54.06 75.44 68.64 72.35 60.98 97.29 54.50 76.33 74.48 68.46 17.32 103.35 73.24 76.81 57.63 72.01 17.34 174.50 45.22 65.89 22.80 175.21 50.56 16.88
Solvent: D2O
Temp:
pD:
Ref: Acetone 31.07 ppm
Comment: 13C-NMR [1]
Non-Sugar Components
- Acetate - (S)-3-hydroxybutyryl - L-Alanine
Enzymes
FlippaseMNNKLARQGLLWSTIERLGTQAIQLLLMLYLGRVLGPSSFGYVGMLTLFLSLAQVLIDSGFSAALIRKSE RTEKDYSTVFIFNICLSILIYIVLYCLSSYIALFYQNTSAGIFYLIFSINRYSKWSNFNPQDSTHVDVNF KIQAKSSLIAITSSSVIAITLAALGYGVWTLVFQSLSYSVINCIVLNIYNPWYPKEKFCYETFKKLFSFS SNLLIAGVLEAFYSNIYQLIIGKFFTPQLVGQFTQANQISSVPAMTLTNIIQRVTYPLFCNIYNGKGKID DAYLNTLKIAGVVVFPILLGIGLISKPLVSVLLGSEWKFTSDILFLLCIAFMIYPIHAININILQVHGRS DLFLRLEILKKTLMTIILVITMQININAMVIGLVLHSYLSWFLNGLFSQKVSTITIRKQLKELLPIWCLC LISGLGSNYIINEISMEPLLNIIFTILLNAIIYITLIRIMYKEIFMTIISLIKNKD
Trembl: A3E2C0
PolymeraseMINLNPVAILTLLYLSINFLSMLIGCSSGEIQVETSIFRVSEESLIYSFLLQAICLIFLYYIYKYFTNRI SYPPLTFKAKWGRALLIIQIAFIIFNTQMGVNTAGSVERIEGQSLSNYLFIILQPDILVAVISVCLNSGF LFWTNILVYLLSMFLRGWMGGTFVILFLILSRYQNLRISLKTFLVSLCSLLLLFSILPALIEAKWAMRTG ISLSVFISNMSSYVTPENYYAGINYLLNRFQHVGHLALIYENADDLFKKYNAGYFSSYYMDGIPQYLLVK MYNLDMYKLSFYLVQYFFDITEPTWNINTGVVGWLYILRYESILFAFYIMLLLLVPYYVVSRFAGKRMLS VLACFSIIYLFHGWLGAYVNLAFYACIISLLANIRLYRTVYIPCEK
Trembl: A3E2C7
Literature
  • First Author: Clark, CG. "Escherichia coli O123 O antigen genes and polysaccharide structure are conserved in some Salmonella enterica serogroups". Journal of Medical Microbiology. 58(Pt . (2009), pp 884-894 PubMed: [19502376]
  • First Author: Beutin, L. "Relationship between O-antigen subtypes, bacterial surface structures and O-antigen gene clusters in Escherichia coli O123 strains carrying genes for Shiga toxins and intimin". Journal of Medical Microbiology. 56. (2007), pp 177-84 PubMed: [17244797]
  • First Author: Perepelov AV. "Relatedness of the O-polysaccharide structures of Escherichia coli O123 and Salmonella enterica O58, both containing 4,6-dideoxy-4-{N-[(S)-3-hydroxybutanoyl]- D-alanyl}amino-D-glucose; revision of t". Carbohydrate Research. 345. (2010), pp 825-829 PubMed: [20181329]