O-Antigen Description

Serogroup:	O112 ac
Serotype H:
Serotype K:
Strain:
Structure:	bDGlcNAc4,6(S)Pyr(1->3) \| ->4)aDGalNAc(1->4)aDGlc(1->4)bDGal(1->3)bDGalNAc(1->
Comment:	- Structure [2] - O-acetylation in bDGlcNAc4,6(S)-Pyr is 70%~ in position 3

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeJ

bDGal(1->

->3)bDGalNAc

GT Name	Donor	Acceptor	Score / e-value
O178-O178_09			272 / 5e-92
O153-O153_06			272 / 5e-92
O5-O05_15			270 / 3e-91
O71-WdaK	bDGal(1->	->3)aDGalNAc	262 / 2e-88
O174-WbcZ			259 / 3e-87
O146-WbwW			257 / 3e-86
O185-O185_03			251 / 5e-84
O171-O171_13			248 / 1e-82
O37-O037_13			244 / 3e-81
O155-WclG			236 / 4e-78
O160-O160_08			236 / 3e-78
O104-WbwC	bDGal(1->	->3)bDGalNAc	231 / 5e-76
O33-O033_09			229 / 4e-75
O70-O070_13			211 / 3e-68
O107-WclZ	bDGal(1->	->3)aDGalNAc	206 / 2e-66
O117-WbeD	bDGal(1->	->3)aDGalNAc	206 / 3e-66
O131-O131_11			191 / 8e-61
O7-WbbD	bDGal(1->	->3)aDGlcNAc	172 / 2e-53
O65-O065_13			156 / 3e-47
O64-O064_03			118 / 7e-33
O28-O028ab_02			116 / 3e-32
O63-WbuP			108 / 3e-29
O74-O074_09			106 / 1e-28
O54-O054_06			103 / 1e-27
O23-O023_06			89 / 1e-22
O114-WbuP	bDGal(1->	->3)aDGlcNAc	88 / 3e-22
O167-WfdH			84 / 1e-20
O22-WbhQ			82 / 5e-20
O92-O092_07			80 / 1e-19

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeK

GT Name	Donor	Acceptor	Score / e-value
O107-WclY			149 / 7e-43
O117-WbeC			145 / 1e-41
O48-O048_07			120 / 7e-33
O119-WekI			98 / 5e-25

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeL

ncbi CAZy

- Reference [1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WfeM

GT Name	Donor	Acceptor	Score / e-value
O124-WfeS			101 / 2e-26
O164-WfeS			101 / 2e-26
O171-O171_11			87 / 1e-21
O144-O144_10			87 / 1e-21
O117-WbeA			84 / 8e-21
O107-WclW			83 / 3e-20
O185-O185_09			82 / 6e-20
O160-O160_05			82 / 6e-20
O112-WfeF			80 / 4e-19

ncbi CAZy

- Reference [1]

NMR
1H-NMR		4.55 3.80 3.55 3.42 3.43 3.78 3.93 5.34 4.20 3.97 4.45 4.05 3.72 3.80 4.89 3.51 3.92 3.64 4.20 3.68 3.88 4.59 3.56 3.73 4.01 3.68 4.96 4.14 3.88 4.14 3.74
Solvent:	D2O
Temp:	55C
pD:
Ref:	Acetone 2.225 ppm
Comment:	1H-NMR [2]
13C-NMR		104.10 57.20 75.10 71.60 77.30 62.50 99.70 50.10 77.50 75.70 72.70 61.50 101.40 73.40 74.90 76.80 72.00 62.20 106.50 72.10 73.50 79.00 76.40 102.40 52.50 82.10 69.80 76.50
Solvent:	D2O
Temp:	55C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [2]

Enzymes
Flippase		MSMLLNLIIPIISFGIVSIMGLIMTPFFINNFGYEGLGLISNSNALIQSLFFAGGAFTTVYSREYSNAVN NIEKSVLLRELNNILTAIFFIAVLLSIVFYIFYIFKDNFSYNYYALVFLASAIIIISQSFNVFPFAANRL YINSVIDCARTLLRNILCMALFYLSIDNVLSNGWAMILSAVITLLVLKLVFKENKVFYVGRMSNNYNIKN SFWVCINQGGAYIFSFADVFLINLMLGHYIGGSYAVLIQIPVLLKSVSVVAIGSISSFTVKVHQRFEKDR TIIKKIINKVVVFFSFFISLAFILVDLYKESFFSIWLPTAVTSSMLKQFNYICIIMMLLSISSLVNIFLV AWGHFIIPAKATVFSAVVSVLVVVLLYFFNYKLSEMIIFSVIYLMLILKNLICYLYFFMQSGLSLIHFIF SLILSFCVITINFLVAKSIRYYIESDFLSIVILFFVMMTISLPLLWDFKKLREYI
Trembl:	B5L3T4
Polymerase		MIKKNNIYRFYILVCVFSGFYIFGFFPAPYYLCLILGFVFLSLSKIKHVDKNILFLLCIVIIYMVFMLFN SRINVWSYYFIGLLTYFIIILLRNNLDYYQKIAIVRKTVLISLLLVILDTIYRFYYPREEYLLAIEQQGK IDYLFYGYKHSFLFQDSNFVGLYILSVFFLFRDNMSIFKWKKSILLTIIILIILSLSRAAIVALILTECI RMLCGMKVDVRLKIVSLIFMAIPTIYLLFVLVANIDDTSFESKFMLLNKFLTTFQERNFTELLFGWGLDN TREHWGIAAHSLFNTLLLEMGLVGFIIITISIVYCWCLNTKTLYHFMGLSIAFLSFGLIFTPFFIPLGLN IIMRDVSNEKL
Trembl:	B5L3T0

Literature
First Author: Liu, B. "Structure and genetics of Shigella O antigens". FEMS Microbiology. 32. (2008), pp 627-653 PubMed: [18422615]
First Author: Perepelov AV. "The O-polysaccharide of Escherichia coli O112ac has the same structure as that of Shigella dysenteriae type 2 but is devoid of O-acetylation: a revision of the S. dysenteriae type 2 O-polysaccharide s". Carbohydrate Research. 343. (2008), pp 977-981 PubMed: [18308298]