O-Antigen Description

Serogroup:	O16
Serotype H:	48
Serotype K:
Strain:	K-12
Structure:	aDGlc(1->6) \| ->2)bDGalf(1->6)aDGlc(1->3)aLRha2Ac(1->3)aDGlcNAc(1->
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbbI

bDGalf(1->

->6)aDGlc

1 | 3

ncbi CAZy

[1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbbK

aDGlc(1->

->3)aLRha

2 | 2

GT Name	Donor	Acceptor	Score / e-value
O109-weiM	aDGlc(1->	->3)aL6dTal	256 / 5e-84
O25-WekC			133 / 3e-37
O123-WfbF			127 / 4e-35
O186-O186_12			127 / 1e-35
O12-O012_05			102 / 9e-27
O172-Orf5			87 / 2e-21

ncbi CAZy

[1]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbbL

aLRha(1->

->3)aDGlcNAc

3 | 1

GT Name	Donor	Acceptor	Score / e-value
O132-O132_08			220 / 4e-72
O48-O048_08			191 / 1e-60
O35-WcnZ			171 / 4e-53
O119-WekH	aLRha(1->	->3)aDGlcNAc	167 / 9e-52
O39-O039_13			165 / 7e-51
O75-wekZ			152 / 5e-46
O139-WfaL	aLRha(1->	->3)aDGlcNAc	133 / 9e-39

ncbi CAZy

[1],[2]

NMR
1H-NMR		5.09 4.18 4.29 4.05 3.91 3.71 4.97 3.56 3.72 3.56 4.02 3.95 4.93 5.17 3.96 3.62 4.13 1.30 4.99 4.20 3.78 3.72 4.02 3.84 5.02 3.59 3.78 3.45 3.78 3.90
Solvent:	D2O
Temp:	30C
pD:
Ref:	Acetone 2.230 ppm
Comment:	1H-NMR [1]
13C-NMR		107.20 87.50 76.80 83.40 71.40 64.20 98.10 72.10 74.10 70.20 71.40 66.70 99.70 69.70 74.50 71.70 69.90 17.70 96.50 54.00 80.80 69.60 72.60 67.40 99.30 72.70 74.30 70.80 73.10 61.80
Solvent:	D2O
Temp:	30C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [1]

Enzymes
Flippase		MNTNKLSLRRNVIYLAVVQGSNYLLPLLTFPYLVRTLGPENFGIFGFCQATMLYMIMFVE YGFNLTATQSIAKAADSKDKVTSIFWAVIFSKIVLIVITLIFLTSMTLLVPEYNKHAVII WSFVPALVGNLIYPIWLFQGKEKMKWLTLSSILSRLAIIPLTFIFVNTKSDIAIAGFIQS SANLVAGIIALAIVVHEGWIGKVTLSLHNVRRSLADGFHVFISTSAISLYSTGIVIILGF ISGPTSVGNFNAANTIRNALQGLLNPITQAIYPRISSTLVLNRVKGVILIKKSLTCLSLI GGAFSLILLLGASILVKISIGPGYDNAVIVLMIISPLPFLISLSNVYGIQVMLTHNYKKE FSKILIAAGLLSLLLIFPLTTLFKEIGAAITLLATECLVTSLMLMFVRNNKLLVC
Trembl:	P37746
Polymerase		MIYLVISVFLITAFICLYLKKDIFYPAVCVNIIFALVLLGYEITSDIYAFQLNDATLIFL LCNVLTFTLSCLLTESVLDLNIRKVNNAIYSIPSKKVHNVGLLVISFSMIYICMRLSNYQ FGTSLLSYMNLIRDADVEDTSRNFSAYMQPIILTTFALFIWSKKFTNTKVSKTFTLLVFI VFIFAIILNTGKQIVFMVIISYAFIVGVNRVKHYVYLITAVGVLFSLYMLFLRGLPGGMA YYLSMYLVSPIIAFQEFYFQQVSNSASSHVFWFFERLMGLLTGGVSMSLHKEFVWVGLPT NVYTAFSDYVYISAELSYLMMVIHGCISGVLWRLSRNYISVKIFYSYFIYTFSFIFYHES FMTNISSWIQITLCIIVFSQFLKAQKIK
Trembl:	P37748

Literature
First Author: Stevenson, G. "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster". Journal of Bacteriology. 176(13. (1994), pp 4144-56 PubMed: [7517391]
First Author: Iguchi A. "A complete view of the genetic diversity of the Escherichia coli O-antigen biosynthesis gene cluster". DNA Research. 22. (2015), pp 101-107 PubMed: [25428893]