O-Antigen Description

Serogroup:O103
Serotype H:2
Serotype K:
Strain:
Structure:
->2)bDGlc(1->2)bDFuc3NBuR3OH(1->6)aDGlcNAc(1->4)aDGalNAc(1->3)bDGlcNAc(1->
Comment:- Structure [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbtD
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbtE

GT NameDonorAcceptorScore / e-value
O70-O070_09


112 / 2e-30
O74-O074_08


102 / 6e-27
O65-O065_08


80 / 3e-19
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbtF

->4)aDGal

GT NameDonorAcceptorScore / e-value
O142-O142_12


362 / 1e-125
O18-wekV

aDGlc(1->

->4)aDGal

357 / 2e-123
O116-O116_10


264 / 1e-87
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

263 / 2e-87
O89-O089_06


212 / 2e-67
O101-O101_06


205 / 1e-64
O121-WbqH


106 / 5e-28
O120-O120_15


95 / 4e-24
O87-WfgO


92 / 5e-23
O51-O051_12


91 / 1e-22
O128-WbsH

aDGal(1->

->3)bDGalNAc

86 / 6e-21
O34-O034_11


86 / 6e-21
O110-O110_02


85 / 9e-21
O65-O065_12


83 / 6e-20
O18-wekW


82 / 1e-19
O22-WbhP


81 / 2e-19
ncbi CAZy
- Reference [1]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbtG

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O101-O101_07


293 / 5e-99
O89-O089_07


288 / 3e-97
O111-WbdH

aDGal(1->

->3)bDGlcNAc

208 / 3e-66
O121-WbqI


167 / 3e-50
O138-WfaH

aDGalNAcA(1->

->3)bDGlcNAc

164 / 6e-49
O10-O010_12


162 / 2e-48
O158-wcmT


152 / 1e-44
O100-O100_11


150 / 6e-44
O133-O133_08

aDGalA(1->

->3)aDGlcNAc

149 / 2e-43
O116-O116_11


140 / 4e-40
O52-Orf16


102 / 1e-26
ncbi CAZy
- Reference [1]
NMR
1H-NMR4.71 3.46 3.49 3.40 3.38 3.75 3.92 4.55 3.90 4.19 3.65 3.85 1.25 2.50 4.25 1.28 4.95 3.96 3.87 3.86 4.21 3.93 4.03 2.09 5.49 4.27 3.91 4.06 3.92 3.71 3.75 2.07 4.82 3.76 3.75 3.69 3.40 3.78 3.93 2.05
Solvent: D2O
Temp: 67C
pD:
Ref: TSP 0 ppm
Comment: 1H-NMR [2]
13C-NMR101.10 82.30 77.20 71.10 76.80 62.20 103.50 74.20 55.60 71.30 72.60 16.20 174.30 45.80 65.80 23.20 99.20 54.90 71.30 70.10 72.10 68.80 22.80 175.00 98.00 50.70 68.00 77.70 72.60 60.90 22.90 175.30 102.20 55.50 79.40 71.70 76.60 61.80 23.40 174.90
Solvent: D2O
Temp: 67C
pD:
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [2]
Non-Sugar Components
- (R)-3-hydroxybutyryl
Enzymes
FlippaseMEKLNFAKVTILSGVVTCFRLICGLLISKVVAIYTGPIGIANLGQLQNFVAFINGFISSQ VSQGVNRYSAENQGDYENACFYWRASIKLSFIACCFIISTGVLFSSKISEYLFYQSDLYW LIIIALCALPLNIVNNIFLGVLNGLNEYNRFFLANVLAISSSLLSMVGLVYFFGLKGALV SASLNNAVAGVWLITIIIKRPWFKFKYWVGHTPRHNITQMKNYFYMGVIGALTGPISMIV VRTILTNNFSLEDAGYWQAVNRISEAYLAVLTTALTVYYFPKTAAARRYSEYITLLKTGA CIVVPLALSMALTIYGLKDFIISILFTADFIRARELFLFQNIGDFLRICSWLFATILLAK GYFKINAILEVMFSIIFPVLVKFLIINFGLNAVSIAYSITYALYFIVVCIIYCWHLTKKA
Trembl: Q6QNC5
PolymeraseMYILSMAIPLFFISLCWHRFSHQTQVAFRLLFIVFFSLSFFSYYTNGVDWSVYYLRFIED EGLFTSFEFGFVIILKLLLIVSDDNFGLAILLYYFLCFVLLSLILKKYKVNEPLFLGCLL LLFGYNLFLEQLRQLLACIIVFYAMLLYNQNKNLIELCILVITASTFHVSAVIILPTIFL ISFRNVTTFIIITVSSITSIVVFMIALYSAISSLAAMSFVFAKIDYYLHQYPVVLNFGWL NILDALFILFYMRYRNAIDRQININILTRIIFVGSVIHLFSGSITFLARVCFYFYFVGVY IYCYSLNDKTPRVFAIKNFNTLVLAAFVNVVLLLNFASYFRNEQAPVGFYNMNFQFFNTF NDNYLRTNAYDKYFKGMQGSVD
Trembl: Q6QNC2
Literature
  • First Author: Fratamico, PM. "DNA sequence of the Escherichia coli O103 O antigen gene cluster and detection of enterohemorrhagic E. coli O103 by PCR amplification of the wzx and wzy genes". Canadian Journal of Microbiology. 51(6). (2005), pp 515-22 PubMed: [16121232]
  • First Author: Liu B. "Genetic and structural relationships of Salmonella O55 and Escherichia coli O103 O-antigens and identification of a 3-hydroxybutanoyltransferase gene involved in the synthesis of a Fuc3N derivative". Glycobiology. 20(6). (2010), pp 679-688
  • First Author: Vinogradov E. "Structural determination of the O-antigenic polysaccharide of enteropathogenic Escherichia coli O103:H2". Canadian Journal of Microbiology. 56(5). (2010), pp 66-72 PubMed: [20555398]