O-Antigen Description

Serogroup:O145
Serotype H:
Serotype K:
Strain:
Structure:
->4)aNeu5Ac(2->3)aLFucNAm(1->3)bDGlcNAc(1->
Comment:- Structure [1]
- LFucNAm=2-acetamidoylamino-2,6-dideoxy-L-galactose
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbuW

aNeu5Ac(2->

->3)aLFucNAm

1 | 2
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbuB

aLFucNAm(1->

->3)bDGlcNAc

2 | 1
GT NameDonorAcceptorScore / e-value
O15-WbuB


701 / 0.0
O151-WbfG


665 / 0.0
O118-WbfG


665 / 0.0
O29-WbuB


629 / 0.0
O108-O108_18


590 / 0.0
O4-WbuB

aLFucNAc(1->

->3)bDGlcNAc

550 / 0.0
O177-WbuB


548 / 0.0
O25-WbuB


548 / 0.0
O172-WbuB

aLFucNAc(1->

->3)aDGlcNAc

536 / 0.0
O12-O012_09


509 / 0.0
O26-WbuB

aLFucNAc(1->

->3)bDGlcNAc

494 / 0.0
O98-WbwW

aLQuiNAc(1->

->3)bDGlcNAc

443 / 2e-157
O181-O181_07


380 / 1e-132
O123-WbwH


370 / 2e-128
O186-O186_16


333 / 5e-114
O163-O163_11


317 / 5e-108
NMR
1H-NMR4.56 5.17 1.89 2.41 1.19 1.98 2.06 2.30 3.72 4.10
Solvent: D2O
Temp: 50C
pD:
Ref: Acetone 2.225 ppm
Comment: Partial NMR data from [1]
13C-NMR97.30 97.80 103.00 177.60 40.60 16.80 62.00 64.60 68.40 51.70 54.30 57.00 23.70 174.70 175.20 20.50 167.70
Solvent: D2O
Temp: 50C
pD:
Ref: Acetone 31.45 ppm
Comment: Partial NMR data from [1]
Enzymes
FlippaseMFNTMLKYYSSVGLRGITLLTKFIFIVLLARLLPSTDLGVYGLINAAVGYGIFVVGFEFY TYSTREIINSQKNRLFFILKNQALFTVISYILCIPAFIFLLYLEILPSGSEYWFILLLFF EHLSQEINRVLITIESQSIASFILFVRQGVWCWLAIAVMLVYPNLRNITVVFIFWFGGTV SASVLGVAYILNKKKQSDITNWDWTWIKKGIKLSVPMLIAALALRGFFTFDRFAVEKISG LEVLGGYTLFVSMTSAIQSFLDTILISFSFPKLALLYSGKKYIKFKSELRKFTYKLILLL SFLSICCFFTGIILVKWLDKRDYIQLFPVFILLIAATYIYCISLIPHIALYAMREDRYIL VSQLISFLSFLLFVFFSVYQSDIYYLLIGMIASFVLLLILKMIPLYKILKKV
Trembl: Q5ISX6
PolymeraseMNIKKDKFINGVIFFWLIISSLYYLNAIFSGVDTLKYNEDLTQKIIKYIVCLVISLSILF IYKKFNYFFVLFFFLFLSVASALFSGAVTIYATTMLIIATMISFCLIIPLFSYNMVKVNR VLLWTGVIVGTISVLELTVFYNYMVSYWAATDGIRSISSLLNPTNSGAYSAIIILIALVT NIKSLFKRALFLIMPMITLISSGSRTAWLSLGMTLLLTVVLRDSASIRLRKKIFTLASIG TVCGALYAIFYMGSISGIESQYRGLNTYTASIRVENFLTYLNLVDLNMLLPDFLDKNINL ISDNFYLVMFNYAGLIGFFIVLLILLLLIFWNIQFKIFNELMAEDIAIWRVVFIYFLISG LSNSFINSFPVNQLFFISCGYYIYKYKLVKSSIGR
Trembl: Q5ISX5
Literature
  • First Author: Feng, L. "Structural and genetic characterization of enterohemorrhagic Escherichia coli O145 O antigen and development of an O145 serogroup-specific PCR assay". Journal of Bacteriology. 187(2). (2005), pp 758-64 PubMed: [15629947]