O-Antigen Description

Serogroup:O5 ac
Serotype H:
Serotype K:
Strain:
Structure:
->2)bDQui3NAc(1->3)bDRibf(1->4)bDGal(1->3)aDGalNAc(1->
Comment:- Structure [1]
- Differs from other O5 structure in the position of the linkage between GalNAc and Qui3NAc [1,2]
NMR
1H-NMR5.38 4.41 3.74 4.21 3.86 3.79 3.79 5.26 4.30 4.27 4.09 3.62 3.72 4.76 3.59 3.95 3.29 3.59 1.34 4.44 3.53 3.75 4.03 3.73 3.74 3.77 2.06 2.07
Solvent: D2O
Temp: 45
pD:
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1]
13C-NMR96.30 48.60 78.40 69.50 71.80 61.60 109.30 75.30 78.90 81.70 63.10 103.50 73.60 56.30 73.60 74.10 17.80 105.90 71.50 73.60 76.60 75.30 61.90 23.10 174.80 23.30 175.30
Solvent: D2O
Temp: 45
pD:
Ref: Dioxane 67.4 ppm
Comment: 13C-NMR [1]
Literature
  • First Author: MacLean, LL. "Structural characterization of the serotype O:5 O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli O:5.". Biochemistry and Cell Biology. 75(3). (1997), pp 199-205 PubMed: [9404639]
  • First Author: Urbina, F. "Structural elucidation of the O-antigenic polysaccharide from the enteroaggregative Escherichia coli strain 180/C3 and its immunochemical relationship with E. coli O5 and O65". Carbohydrate Research. 340(4). (2005), pp 645-650 PubMed: [15721335]
  • First Author: Sarkar A. "Conformational preferences of the O-antigen polysaccharides of Escherichia coli O5ac and O5ab using NMR spectroscopy and molecular modeling". Biomacromolecules. 14(7). (2013), pp 2215-24 PubMed: [23721050]