O-Antigen Description

Serogroup:	O114
Serotype H:	2
Serotype K:
Strain:
Structure:	->4)bDQui3NLSerAc(1->3)bDRibf(1->4)bDGal(1->3)aDGlcNAc(1->
Comment:	- Structure [1] - SerAc=N-Acetyl-Seryl

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbuL

GT Name	Donor	Acceptor	Score / e-value
O63-WbuL-like protein			86 / 5e-21

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbuM

GT Name	Donor	Acceptor	Score / e-value
O5-O05_12			189 / 2e-59
O55-WbgO			90 / 9e-23
O55-WbgO			90 / 9e-23

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbuP

bDGal(1->

->3)aDGlcNAc

3 | 1

GT Name	Donor	Acceptor	Score / e-value
O63-WbuP			317 / 1e-109
O64-O064_03			243 / 7e-81
O54-O054_06			239 / 5e-79
O92-O092_07			184 / 5e-58
O74-O074_09			154 / 4e-46
O160-O160_08			107 / 8e-29
O174-WbcZ			102 / 4e-27
O117-WbeD	bDGal(1->	->3)aDGalNAc	100 / 3e-26
O107-WclZ	bDGal(1->	->3)aDGalNAc	99 / 8e-26
O70-O070_13			99 / 4e-26
O171-O171_13			96 / 8e-25
O7-WbbD	bDGal(1->	->3)aDGlcNAc	95 / 1e-24
O155-WclG			93 / 6e-24
O55-WbgO			92 / 2e-23
O55-WbgO			92 / 2e-23
O178-O178_09			91 / 4e-23
O153-O153_06			91 / 4e-23
O104-WbwC	bDGal(1->	->3)bDGalNAc	89 / 2e-22
O112-WfeJ			88 / 3e-22
O185-O185_03			88 / 4e-22
O71-WdaK	bDGal(1->	->3)aDGalNAc	87 / 5e-22
O37-O037_13			87 / 6e-22
O65-O065_13			86 / 2e-21
O146-WbwW			84 / 5e-21
O167-WfdH			82 / 7e-20
O5-O05_15			81 / 1e-19
O23-O023_06			81 / 6e-20
O127-WbiP			81 / 7e-20
O90-O090_13			81 / 7e-20

ncbi CAZy

- Reference [2] [3]

NMR
1H-NMR		5.31 5.26 4.60 4.53 2.09 2.05 1.39
Solvent:	D2O
Temp:	70C
pD:
Ref:	TSP 0.0 ppm
Comment:	1H-NMR [1]
13C-NMR		110.62 74.94 74.72 81.92 62.70 98.04 105.60 105.30 54.00 57.95 58.95 20.70 176.36 174.00
Solvent:	D2O
Temp:	70C
pD:
Ref:	TSP 0.0 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- Acetate - Serine

Enzymes
Flippase		MKKLLTVTFFTGMLTFFKMISGFIISKIVAIYTGPSGIVLFGQIQSIVSALNGVINSPVG PGIVRFTAENHRKGLEHCYKWWIAGLSWVCIISAIIIPLSITLSSHIAFWTLGSEKYSWV IILTACLLPLTAIGTFISSVINGFQNYKVYVLLGIISAFLSLIIMIALIVFGKINGALLA ISVQNALIGGVMIIYASSQPWFNLRWLYGRVHKTYYKDIQRYIVMAITSAIVVPGSLIIV RNIIIHYVGWTEAGYWQAVWKISETYLAIITIALSTYYLPRLSSLSQKDDIIKEISSSLK IIIPLVMFFSMGIYLSRDFIISLLFTKEFLGARMLFQVQLIGDILKIASWLYAYVMISKG MTQLFVISEVLFSLLFISFSLVFIRCYGLIGVTYAYCLNYFIYLVFSVVVVKRAKYD
Trembl:	Q697E1
Polymerase		MVTLFMEIILFISLSLFLILVYRVIANFSGVLALDFISFWFYTYIVFAFLGSLIIITGFG DDSYFVQPLSAHARNVFFGGSLMVLYAGLGTFSIFLLVLSLFLGKHRYKEKLIEKCTSES YDKITLAMLFIILMLVFFYYLISTFPSPLYLGLINASSEDIALRRIEVTKNLSNIANTYI ISIGMLMSYIVSYAYLALFSIHKKYPIRTFLSVLIASIFILSTGEKGPFLFYILGALICC SYAQGKIRRVSIKLIAITLFFLFLIYFIFVSSELSMITALIFDRIFIAQVISVYLSFDFY SNLGDIGFASLSNVFTKLYDSKSVMPASEELMSVYYPEMLKLGGWNVNGLFISEAWANFG VLGAIISPVIVGIENGILLSILMKIKKSPMQCAFYAYFTVKCVYFLTSFNAYLYHSDWIV CLLVLVLICFLNAIGKHFILKYK
Trembl:	Q697D9

Literature
First Author: Dmitriev, BA. "Cell-wall lipopolysaccharide of Escherichia coli 0114:H2. Structure of the polysaccharide chain". European Journal of Biochemistry. 134. (1983), pp 517-521 PubMed: [6309515]
First Author: Feng, L. "Identification of Escherichia coli O114 O-antigen gene cluster and development of an O114 serogroup-specific PCR assay". Journal of Clinical Microbiology. 42. (2004), pp 3799-3804 PubMed: [15297533]
First Author: Zhou D. "Biochemical characterization of a new β-1,3-galactosyltransferase WbuP from Escherichia coli O114 that catalyzes the second step in O-antigen repeating-unit". Carbohydrate Research. 381. (2013), pp 43-50 PubMed: [24056013]