O-Antigen Description

Serogroup:O119
Serotype H:
Serotype K:
Strain:
Structure:
aLRhaNAc3NFo(1->3)
                |
         ->2)bDMan(1->3)aDGal(1->4)aLRha(1->3)aDGlcNAc(1->
Comment:- Structure [1]
- LRhaNAc3NFo=2-acetamido-2,3-dideoxy-3-formamido-L-rhamnose
- Different absolute configurations for aLRhaNAc3NFo have been reported where the L-configuration is concluded in [2] - A Study of the Biosynthesis of E. coli O119 components has been published [3]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WekH

aLRha(1->

->3)aDGlcNAc

1 | 1
GT NameDonorAcceptorScore / e-value
O48-O048_08


251 / 2e-84
O35-WcnZ


222 / 6e-73
O75-wekZ


212 / 4e-69
O39-O039_13


201 / 8e-65
O139-WfaL

aLRha(1->

->3)aDGlcNAc

186 / 3e-59
O132-O132_08


171 / 4e-53
O16-WbbL

aLRha(1->

->3)aDGlcNAc

167 / 9e-52
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WekI

GT NameDonorAcceptorScore / e-value
O107-WclY


148 / 1e-42
O117-WbeC


144 / 3e-41
O48-O048_07


134 / 1e-37
O112-WfeK


98 / 5e-25
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WekJ

aLRhaNAc3Fo(1->

->3)bDMan

3 | 4
GT NameDonorAcceptorScore / e-value
O182-O182_07


174 / 1e-52
O3-WclS


132 / 7e-37
O109-weiN

bLRhaNAc3NAc(1->

->3)aDGlc

114 / 1e-30
ncbi CAZy
- Reference [2]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WekK

bDMan(1->

->3)aDGlcNAc

GT NameDonorAcceptorScore / e-value
O179-O179_08


90 / 3e-22
O106-WbaD


86 / 1e-20
O73-WbaD

bDMan(1->

->3)aDGlcNAc

86 / 1e-20
O77-WbaD

bDMan(1->

->3)aDGlcNAc

86 / 1e-20
O59-WclC


84 / 3e-20
ncbi CAZy
- Reference [2]
NMR
1H-NMR8.05 5.31 5.07 4.98 4.94 4.86 4.53 4.45 3.31 2.02 1.33
Solvent: D2O
Temp: 27C
pD:
Ref: Acetone 2.225 ppm
Comment: 1H-NMR [1]
13C-NMR175.54 174.15 168.23 165.16 102.16 101.85 101.64 100.55 97.50 53.94 53.04 52.15 22.87 22.56 17.79 17.64
Solvent: D2O
Temp: 27C
pD:
Ref: Acetone 31.07 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMVISNKANIASNYLSKLWGVLSIYIFIPFYISILGEENYALIAFQSVILSFVFIADGGLG AAFSRECAKEKNRDCLKALLFSVEKVMFIILLIIASIFYFCSNLIASKWLKIENIDFLSS AVISLKLMAICIIPQVMLTLYYGGIMGLEKQVLANIINICYTFCRSGLVLVPIFFYKDVV LYFGWYLIVSFLFVFIFRWCLLRQLNLVKKPAIVDNADAIDGFSILKGLFSFSFGMFCLS IVSAVNNQLDKLYVSYEFSLNDFTFYNISAILGQSAFLLVLPLAVTVLPALTKLVGGEGD AGLRDYTYERFSYIVSALSSICVFSLIYYLNDIIKIWLGDVYLNSSIEDLVPLLSIGTLF LCLQLIPFQLSIANGYNKLSVRLGFFSLAIYPLLLVYFCKVLGVTGATIPWVVINALNFL VLTFFVNTRFYTGCKKWMLLMCLLPVTISFILISLGCLLANILQLDYVLHFSMGVIFGVV AILI
Trembl: D2X3E0
PolymeraseMIQRSLGARTCLYIFLFTAFVSYLNALLGGKFNGDFSGVNINLDSIQLLGVFILTCAPFL FLWYVYSLFNRIKFKELDAGQLGFRVSVFFKLFVFWSFFVTINYGVGIMARSEYNVPAAI SYIIYFTNRIDVFYLGVLFILLYQGRVLFFWIFILCVLGVIRGGIGVFLYVSIALILRYN ITLGKFFFRRPLLCFLALIISPFVVDSLFYIRELLRGDYINEQFTFYQLIIGKLIGRFSS FSNLGMIFQNEGYFLQNIFIMDQFYFVKHFFSAFIGQSIIPDVIPERLLINIFGGDLFDK SYMVGLSGNMYISSMISPTIMMINLILIFISVICTFIVAGLIGFKYSREYAFALLLYPCM SGSAQEFAKLLLSMLFIMLILASCNIKLKIKRGFARGERV
Trembl: D2X3E2
Literature
  • First Author: Anderson, AN. "Structure of the O-antigen of Escherichia coli 0119 lipopolysaccharide". Carbohydrate Research. 237. (1992), pp 249-262 PubMed: [1284112]
  • First Author: Liu B. "Development of a serogroup-specific DNA microarray for identification of Escherichia coli strains associated with bovine septicemia and diarrhea". Veterinary Microbiology. 142. (2010), pp 373-378 PubMed: [19932572]
  • First Author: T Jia. "Biosynthesis of UDP-2-acetamido-4-formamido-2,4,6-trideoxy-hexose by WekG, WekE, WekF, and WekD: Enzymes in the Wek pathway responsible for O-antigen Assembly in Escherichia coli O119". Carbohydrate Research. 507. (2021), pp 108388