O-Antigen Description

Serogroup:O18 ac
Serotype H:
Serotype K:
Strain:
Structure:
->2)aLRha(1->6)aDGlc(1->4)aDGal(1->3)aDGlcNAc(1->
                             |
                 bDGlcNAc(1->3)
Comment:- Structure [1], revised from [2]
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
wekT

aLRha(1->

->6)aDGlc

GT NameDonorAcceptorScore / e-value
O142-O142_10


365 / 3e-127
O34-O034_10


156 / 2e-46
O107-WclX

aLRha(1->

->4)aDGlcNAc

143 / 2e-41
O117-WbeB

aLRha(1->

->4)aDGlc

142 / 5e-41
O4-WbuG

aLRha(1->

->6)aDGlc

120 / 4e-33
O180-wfcT


105 / 5e-28
O10-O010_11


95 / 4e-24
O51-O051_11


94 / 5e-24
O154-O154_12


90 / 2e-22
O100-O100_10


90 / 1e-22
O97-O097_05


88 / 1e-21
O154-O154_14


88 / 6e-22
O129-RfbG


87 / 1e-21
O135-RfbG


87 / 1e-21
O13-RfbG

aLRha(1->

->3)bDGlcNAc

87 / 1e-21
O38-O038_09


87 / 7e-22
O165-WcnW


83 / 3e-20
O82-O082_09


80 / 2e-19
ncbi CAZy
- Reference [4]
GT nameDonorAcceptorPosition | Link.NumSimilar to
wekU

bDGlcNAc(1->

->3)aDGal

2 |
GT NameDonorAcceptorScore / e-value
O142-O142_11


290 / 3e-99
O3-WclU


259 / 2e-87
O38-O038_06


144 / 1e-42
O23-O023_03


99 / 5e-26
ncbi CAZy
- Reference [4]
GT nameDonorAcceptorPosition | Link.NumSimilar to
wekV

aDGlc(1->

->4)aDGal

3 |
GT NameDonorAcceptorScore / e-value
O103-WbtF


352 / 9e-122
O142-O142_12


343 / 2e-118
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

281 / 6e-94
O116-O116_10


261 / 3e-86
O101-O101_06


195 / 1e-60
O89-O089_06


194 / 3e-60
O22-WbhP


101 / 4e-26
O87-WfgO


100 / 2e-25
O120-O120_15


93 / 2e-23
O121-WbqH


92 / 7e-23
O51-O051_12


88 / 1e-21
O110-O110_02


86 / 5e-21
O113-WbnE


82 / 3e-19
ncbi CAZy
- Reference [4]
GT nameDonorAcceptorPosition | Link.NumSimilar to
wekW

aDGal(1->

->3)aDGlcNAc

GT NameDonorAcceptorScore / e-value
O69-O069_12


278 / 9e-93
O3-WclR


272 / 6e-90
O110-O110_02


268 / 9e-89
O40-WffO


265 / 4e-87
O113-WbnE


263 / 1e-86
O55-WbgM


243 / 3e-79
O55-WbgM

aDGal(1->

->3)bDGalNAc

239 / 1e-77
O128-WbsH

aDGal(1->

->3)bDGalNAc

236 / 1e-76
O87-WfgO


172 / 1e-51
O120-O120_15


150 / 7e-44
O51-O051_12


149 / 3e-43
O148-WbbG

aDGlc(1->

->3)aDGlcNAc

142 / 2e-40
O22-WbhP


139 / 2e-39
O19-O019_11


136 / 2e-38
O116-O116_10


94 / 8e-24
O76-O076_08


94 / 1e-23
O121-WbqH


90 / 2e-22
O142-O142_12


87 / 2e-21
O101-O101_06


87 / 4e-21
O89-O089_06


86 / 6e-21
O111-WbdM

aCol(1->

->3)aDGlc ->6)aDGlc

83 / 6e-20
O138-WfaH

aDGalNAcA(1->

->3)bDGlcNAc

83 / 8e-20
O142-O142_03


82 / 3e-19
O65-O065_12


81 / 4e-19
ncbi CAZy
- Reference [4]
NMR
1H-NMR5.47 3.95 3.81 5.02 4.09 4.01 4.93 3.49 3.70 4.85 4.02 3.92 4.64 3.60 3.58
Solvent: D2O
Temp: 60C
pD:
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1]
13C-NMR175.20 174.70 104.20 99.90 99.70 98.80 96.80 79.30 77.70 76.60 76.60 76.60 75.10 73.70 73.10 72.80 72.80 72.60 71.40 71.40 71.10 70.40 70.00 69.70 68.90 67.40 62.00 61.20 60.60 56.70 52.80 23.10 23.10 17.90
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 31.00 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMSLIKNSFWNLCGYVLPAIVTLPALGIMGRKLGPELFGVFTLALAVVGYASIFDAGLTRA VIREVAIEKDNEENKLKIISSATVVIIYLSLAASLLLFFFSGHIALLLNISETFFHNVSV SLKILAASIPLFLITQIWLSILEGEERFGLLNIYKSITGVILAISPALFILIKPSLMYAI IGLVLARFLCFILAFIICHDKVLKAKLTIDIPTIKRLFMFGGWITVSNIISPVLSYFDRF IVSNQLGAANVAFYTAPSEIISRLSIIPGAFSRALFPRLANANNSAERYKTKRLITISLL IIITPIFCIGVLFSEKIMVLWMGASFFGEPGLVLSILLIGFIFNGLAQVPFASIQSRGHA KITAFVHLLELFPYLLLLFYLIKAHGVVGAGIAWSVRMIVDYIALSLLDGKYINK
Trembl: D7PDW3
PolymeraseMIYILTLTLLLVIAIMFSLLGTKSRITSPLPLHFLPWLLTLIVGISNYDQFYEFNERSFY SLLIWFTVIFIFYFIGELVNYKRENINVYYGLSHIKYECKKYWIIVIPISLYTIFEIYMV GMGGADGFFLNLRLANTLEGYTGKKFILMPAVYPLMMAMFAIVCLTKTSKLNKYSIYFWM FLYCIGTMGKFSILTPILTYLIIYDFKHRLKVKKTIKFTLLIIILALTLHFTRMAENDHS TFLSILGLYIYSPIIALGQLNEVNSSHFGEYTFRFIYAITNKIGLIKELPVNTILDYSYV PVPTNVYTALQPFYQDFGYTGIIFGAVLYGLIYVSLYTAGVRGNNTQALLIYALFSVSSA TAFFAETLVTNLAGNVKLVLCTILLWRFTVICKPAQ
Trembl: D7PDW4
Literature
  • First Author: Jann, B. "The O18 antigens (lipopolysaccharides) of Escherichia coli. Structural characterization of the O18A, O18A1, O18B and O18B1-specific polysaccharides". European Journal of Biochemistry. 210(1). (1992), pp 241-8 PubMed: [1280216]
  • First Author: Oxley, D. "Structure of the O-specific polysaccharide from the lipopolysaccharide of Serratia marcescens O8". European Journal of Biochemistry. 156(3). (1986), pp 597-601 PubMed: [2422032]
  • First Author: Jansson, PE. "Structure of the O-antigen polysaccharide from Escherichia coli O18ac: a revision using computer-assisted structural analysis with the program CASPER". Carbohydrate Research. 193. (1989), pp 322-325 PubMed: [2482127]