O-Antigen Description

Serogroup:O25
Serotype H:
Serotype K:
Strain:F171
Structure:
bDGlc(1->6)
         |
  ->4)aDGlc(1->3)aLFucNAc(1->3)bDGlcNAc(1->
         |
aLRha(1->3)
Comment:- Structure [1]
- Strains are F171 for structure and G1111/G1249 for GT data
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WekA

GT NameDonorAcceptorScore / e-value
O68-O068_07


264 / 1e-87
O62-O062_09


264 / 1e-87
O141-WfcB


261 / 3e-86
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WekB

GT NameDonorAcceptorScore / e-value
O158-wcmS


251 / 1e-81
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WekC

aDGlc(1->

GT NameDonorAcceptorScore / e-value
O123-WfbF


278 / 1e-92
O186-O186_12


263 / 1e-86
O172-Orf5


160 / 3e-47
O12-O012_05


135 / 4e-38
O16-WbbK

aDGlc(1->

->3)aLRha

133 / 3e-37
O109-weiM

aDGlc(1->

->3)aL6dTal

115 / 5e-31
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbuB

aLFucNAc(1->

->3)bDGlcNAc

GT NameDonorAcceptorScore / e-value
O177-WbuB


706 / 0.0
O4-WbuB

aLFucNAc(1->

->3)bDGlcNAc

705 / 0.0
O172-WbuB

aLFucNAc(1->

->3)aDGlcNAc

691 / 0.0
O12-O012_09


647 / 0.0
O26-WbuB

aLFucNAc(1->

->3)bDGlcNAc

629 / 0.0
O29-WbuB


556 / 0.0
O15-WbuB


549 / 0.0
O145-WbuB

aLFucNAm(1->

->3)bDGlcNAc

548 / 0.0
O151-WbfG


523 / 0.0
O118-WbfG


523 / 0.0
O108-O108_18


519 / 0.0
O98-WbwW

aLQuiNAc(1->

->3)bDGlcNAc

453 / 2e-161
O181-O181_07


383 / 7e-134
O123-WbwH


378 / 2e-131
O186-O186_16


339 / 2e-116
O163-O163_11


326 / 2e-111
ncbi CAZy
- Reference [3]
NMR
1H-NMR5.22 4.03 3.91 3.49 4.40 1.35 5.01 3.64 3.95 3.85 3.90 3.75 4.16 4.98 4.35 3.95 3.88 4.42 1.17 4.55 3.31 3.52 3.41 3.48 3.75 3.95 4.54 3.89 3.66 3.47 3.45 3.78 3.96
Solvent: D2O
Temp: 35C
pD:
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1]
13C-NMR101.50 71.00 71.00 73.20 69.50 18.00 101.00 73.30 76.70 73.60 71.00 67.70 99.10 49.20 76.70 72.00 67.70 16.40 103.00 73.90 76.80 70.60 76.90 61.70 101.20 56.60 79.90 69.70 77.00 62.20
Solvent: D2O
Temp: 35C
pD:
Ref: Dioxane 67.40 ppm
Comment: 13C-NMR [1]
Slightly different shifts are given in [2]
Enzymes
FlippaseMSLLKHSIWNVAGYFIPTLIAIPAFGLIAREIGVELFGLYTLSMIFIGYASIFDAGLTRA VVREIALLKNRLDDCNTIIVTSIIAVVFLGGIGGGGVFLLKDHIIELLNMSPIYYVDAIK SLILLSSLIPVFLVTQILLAELEGREYFGILNIQKSLGNSLIAGLPALFVLINQTLFSAI IGVAIARVICLWLSYIMSRKRITIDISFFSITVLKRLFRYGGWVTISNIISPILASMDRF ILSHIQGASKISFYTVPNELVTRLGIVPGSLGKAVFPKLSHASSFTASYAEQKKAYILMT VIVLPLVLFIYYYAKFILTLWMGAEYAGISVEILRIMLIGYIFNCYSQISFANIQAFGKA KYTAYIHMMEFIPYLIMLYIISKECGVIGVAWLWTIRVIIDFLMLFYMSYRCNNLMKKG
Trembl: D7P6C4
PolymeraseMSIRIEEPNSTKRIIFLFLLFLVFPDFLFYTLGIDNFSISTIISIILLFVFLRAKNTCKD NLLIIVVLFILLCFNCLLSMLFNIEQVLSFKIVLSMYSILIMAYVSSCYAQTLWLCSEEI LKRSVFYLFAFLCLIGIISILLQKTEIIHDKSMILFPEPSAFALVFIPIFSFCLYYTRGG GVLLLYILSLGIALGIQNLTMLVGIVISVFVMKKITIRQTIVIFLGAWIFSMILSDLDIS YYTSRLDFKNTTNLSVLVYLSGIERAFLNFITSYGLGIGFQQMGVNGEVGVYQQILADLD APMLNIYDGSFISSKLISEFGFIGAIMCIFYLFIFFDFICVSKKIRDIHRSIF
Trembl: D7P6C7
Literature
  • First Author: Fundin J. "NMR analysis of the O-antigen polysaccharide from Escherichia coli strain F171". Magnetic Resonance in Chemistry. 41. (2003), pp 202-205
  • First Author: Kenne L. "Structural Studies of the Escherichia coli O-antigen 25". Carbohydrate Research. 122. (1983), pp 249-256 PubMed: [6200224]
  • First Author: Wang Q. "Development of a DNA Microarray for Detection and Serotyping of Enterotoxigenic Escherichia coli". Journal of Clinical Microbiology. 48(6). (2010), pp 2066-74 PubMed: [20351209]