O-Antigen Description

Serogroup:O127
Serotype H:
Serotype K:
Strain:
Structure:
->2)aLFuc(1->2)bDGal(1->3)aDGalNAc(1->3)aDGalNAc(1->
Comment:- Structure [1]
- aLFuc is O-Acetylated in position 3 and 4 in non-stoichiometrical amounts (65% and 35% respectively)
GTs
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbiN

aDGalNAc(1->

GT NameDonorAcceptorScore / e-value
O86-WcmA

aDGalNAc(1->

->3)bDGalNAc

655 / 0.0
O86-WbnH


654 / 0.0
O86-WbwH


654 / 0.0
O90-O090_04


650 / 0.0
O142-O142_03


358 / 3e-124
O76-O076_08


282 / 9e-95
O120-O120_15


128 / 1e-35
O87-WfgO


111 / 1e-29
O22-WbhP


105 / 1e-27
O69-O069_12


85 / 1e-20
O55-WbgM


82 / 9e-20
O128-WbsH

aDGal(1->

->3)bDGalNAc

81 / 3e-19
O110-O110_02


81 / 3e-19
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbiP

bDGal(1->

->3)aDGalNAc

GT NameDonorAcceptorScore / e-value
O90-O090_13


503 / 0.0
O86-WbnJ

bDGal(1->

->3)aDGalNAc

305 / 1e-105
O86-WcmC

bDGal(1->

->3)aDGalNAc

305 / 1e-105
O86-WbwJ


304 / 2e-105
O23-O023_06


251 / 1e-84
O126-WbsT


147 / 4e-44
O55-WbgO


137 / 3e-40
O55-WbgO


137 / 3e-40
O167-WfdH


119 / 4e-33
O128-WbsK

bDGalNAc(1->

->4)aDGal

117 / 9e-33
O74-O074_09


96 / 8e-25
O113-WbnA


96 / 6e-25
O65-O065_13


95 / 9e-25
O54-O054_06


95 / 1e-24
O63-WbuP


94 / 2e-24
O5-O05_15


93 / 7e-24
O185-O185_03


92 / 8e-24
O171-O171_13


91 / 4e-23
O160-O160_08


91 / 2e-23
O37-O037_11


88 / 3e-22
O146-WbwW


86 / 1e-21
O33-O033_09


86 / 2e-21
O178-O178_09


85 / 3e-21
O153-O153_06


85 / 3e-21
O104-WbwC

bDGal(1->

->3)bDGalNAc

85 / 4e-21
O125-O125_04


84 / 5e-21
O64-O064_03


82 / 4e-20
O37-O037_13


82 / 4e-20
O7-WbbD

bDGal(1->

->3)aDGlcNAc

81 / 1e-19
O114-WbuP

bDGal(1->

->3)aDGlcNAc

81 / 6e-20
O48-O048_06


80 / 2e-19
O70-O070_13


80 / 1e-19
ncbi CAZy
- Reference [3]
GT nameDonorAcceptorPosition | Link.NumSimilar to
WbiQ

aLFuc(1->

->2)bDGal

GT NameDonorAcceptorScore / e-value
O90-O090_14


620 / 0.0
O86-WbnK

aLFuc(1->

->2)bDGal

293 / 2e-99
O86-WbwK

aLFuc(1->

->2)bDGal

293 / 2e-99
O86-WcmD

aLFuc(1->

->2)bDGal

293 / 2e-99
O55-WbgN


123 / 2e-34
O55-WbgN

aCol(1->

->2)bDGal

123 / 2e-34
O128-WbsJ

aLFuc(1->

->2)bDGal

123 / 2e-34
O126-WbgL


115 / 1e-31
ncbi CAZy
- Reference [3]
NMR
1H-NMR5.34 3.95 3.85 3.69 4.29 1.21 5.24 4.40 3.96 4.14 4.14 3.77 3.77 5.10 4.27 3.97 4.24 3.77 3.77 3.77 4.62 3.75 3.79 3.93 3.67 3.77 3.77 2.05 2.05
Solvent: D2O
Temp: 70C
pD:
Ref: TSP 0.00 ppm
Comment: 1H-NMR [1]
13C-NMR99.17 71.59 70.73 73.27 67.68 16.12 99.47 48.92 74.11 65.54 74.19 62.06 94.03 49.70 75.66 69.73 71.92 61.78 103.15 76.49 74.66 69.95 75.66 61.67 175.50 175.50 23.00 23.00
Solvent: D2O
Temp: 70C
pD:
Ref: Acetone 31.07 ppm
Comment: 13C-NMR [1]
Enzymes
FlippaseMSIISSFKNNIFLRNTMFNYIGMIWMGGGIIVLIPFYIKLLGPHGWGIVSACLSIQAMLL ILDAGCSQIMPRDIAKTNTPENIFFAYFWIYIAVAFVGAVFLTSSAGWLAFRWFNAGHSS RELEFCIRLLAIQFLFQFANNLNIGFWNGTQKQFNTNLSQVCFFSCKHCVALISLMVNPE PIVYIVSFAVVTCIEFIFNFLWITRNNIDLKGLIDNNILIQLIDLVKENYIFSFGVIVGV FVSQLDKILLSHYVETQLYGVYVIVAQLGLSFLQLQYPLMKSVLPALSSQGEKSIFNVLS LKKMIIMLLVLAFPIGLTIIFSSDILYLWTRNHYIANHGGGALALICLSVLINIPYTFIY TNLVRLNAGGIILFSNIISALSMFILFHFMKENNSISIGGMMWIAYASISFSIGGIFLLM RAR
Trembl: Q5J7D0
PolymeraseMMQITIPKSNDFRRFFNPVILILMSIVLNTFFQLYTSEEEWNSFFIFNQKPDYTVFFISL VFYLFFLFGAFFAYLFNYQKHSNHRSHYLYNFSFYNGLLKFVFYSNVISLIVTQYYMHEA NIYNIYISGGVSAQTIESQIASSPLGIHGISVMLGYFGIIMYGISRLIKDNRAIVFISIL VIIIKFISYAKLQSLLYVLLALMLFSRRRISINKGVIIGGVVIFLFSFTRIIRNPEQNLS LSFDFLFRFVGGFYFGSPVVNFNYVIQNNIQDVFYFFNWFLPQKIIPASPISLEFPDVTS PIGFVGSAYVSLGIFSVIYAYLVGFIIQLIYLQRNTSPFAFVFQPFVIMACIFAMMYNNF VNMIFFILPLIFSYLVVIKTICARKEDV
Trembl: Q5J7C8
Literature
  • First Author: Widmalm, G. "Structural studies of the Escherichia coli O127 O-antigen polysaccharide". Carbohydrate Research. 247. (1993), pp 255-262 PubMed: [7693348]
  • Journal of Experimental Medicine. 113. (1961), pp 1077-1093
  • First Author: Yi, W. "Sequence and characterization of Escherichia coli O127 antigen biosynthesis gene cluster (Unpublished)". Not specified. (2003),
  • First Author: Perepelov AV. "The O-antigen of Salmonella enterica O13 and its relation to the O-antigen of Escherichia coli O127". Carbohydrate Research. 345. (2010), pp 1808-11 PubMed: [20594547]