O-Antigen Description

Serogroup:	O22
Serotype H:
Serotype K:	13
Strain:
Structure:	->6)aDGlc(1->4)bDGlcA(1->4)bDGalNAc3Ac(1->3)aDGal(1->3)bDGalNAc(1->
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbhP

aDGal(1->

->3)bDGalNAc

GT Name	Donor	Acceptor	Score / e-value
O120-O120_15			273 / 3e-91
O87-WfgO			248 / 7e-81
O18-wekW			137 / 6e-39
O110-O110_02			135 / 2e-38
O113-WbnE			116 / 3e-31
O128-WbsH	aDGal(1->	->3)bDGalNAc	114 / 2e-30
O55-WbgM			108 / 1e-28
O55-WbgM	aDGal(1->	->3)bDGalNAc	107 / 2e-28
O142-O142_03			107 / 2e-28
O69-O069_12			107 / 2e-28
O86-WbnH			105 / 1e-27
O86-WbwH			105 / 1e-27
O86-WcmA	aDGalNAc(1->	->3)bDGalNAc	105 / 1e-27
O127-WbiN			105 / 1e-27
O90-O090_04			105 / 1e-27
O51-O051_12			104 / 2e-27
O76-O076_08			103 / 5e-27
O116-O116_10			99 / 2e-25
O3-WclR			95 / 4e-24
O89-O089_06			92 / 6e-23
O101-O101_06			91 / 9e-23
O142-O142_12			90 / 2e-22
O18-wekV	aDGlc(1->	->4)aDGal	89 / 6e-22
O148-WbbG	aDGlc(1->	->3)aDGlcNAc	85 / 2e-20
O40-WffO			84 / 4e-20
O19-O019_11			84 / 2e-20
O111-WbdM	aCol(1->	->3)aDGlc ->6)aDGlc	81 / 2e-19

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbhQ

bDGalNAc(1->

GT Name	Donor	Acceptor	Score / e-value
O125-O125_04			105 / 4e-28
O5-O05_15			91 / 5e-23
O128-WbsK	bDGalNAc(1->	->4)aDGal	89 / 2e-22
O167-WfdH			84 / 8e-21
O112-WfeJ			82 / 5e-20
O64-O064_03			82 / 2e-20

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbhR

GT Name	Donor	Acceptor	Score / e-value
O76-O076_07			108 / 6e-29

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbhT

GT Name	Donor	Acceptor	Score / e-value
O111-WbdM	aCol(1->	->3)aDGlc ->6)aDGlc	81 / 2e-19

ncbi CAZy

- Reference [2]

NMR
1H-NMR		5.39 3.43 3.64 3.37 3.76 4.02 3.82 4.43 3.42 3.70 3.75 3.78 4.75 4.17 5.03 4.21 3.71 3.75 3.75 5.06 3.87 3.78 4.15 3.82 3.72 3.72 4.53 4.03 3.80 4.12 3.63 3.70 3.70
Solvent:	D2O
Temp:	80C
pD:
Ref:	Acetone 2.225 ppm
Comment:	1H-NMR [1]
13C-NMR		99.30 73.00 74.10 70.50 72.00 69.10 104.40 74.70 77.40 78.00 77.60 174.30 103.90 52.40 74.20 74.30 75.50 61.90 96.60 68.30 80.90 70.20 72.20 62.30 102.90 52.10 76.80 65.40 76.00 62.10
Solvent:	D2O
Temp:	80C
pD:
Ref:	Acetone 31.45 ppm
Comment:	13C-NMR [1]

Non-Sugar Components
- Acetate

Enzymes
Flippase		MNNNIIDTAGRLKLIAFNFMIFVFLVRVPGFPFIILSIISITILSLFLIKVKVALNNKLIPLYCFVIYMI LRCVIGFVFNGGYEERYFMVLLLMLFVLIVFTVELKDVRLEQCYNGISAAVIVTLLIVLYEIISGNHMST SRYSDINNEAFLWSVSKPTAFYYNENDMLSFVVCFLPFTFVLKNTMLRVAIFLLAAASAIYIGSKAAIIT ILIYGLFTKIISKKAILLLVVTGVVLVNLFATSLIENTSSFISGSSERLLGFWDTILSTRGGDESTSERL SIYKANLDWLGNHPLSATFGNGRFGDYESELMKVYGLRMGEFHNLHLEMITIFGLLFYLLFFSYYFYLWI CLFLKKNKNTACYPLLISSAMYPLIISFGPSSSLKYPFLFILCLLMILGFRNDEKHAKKNI
Trembl:	A7J0Q0
Polymerase		MKINDLLKKYTLFSLSPLLSAFIQLASIFLLGHSLSGPELGIAGIYNTIVFILVFISDGGSSSYFIYRKF LPKYDVNLINIFNVAISAFAIIIIGYYVPEKSDLLGLIVVSLTVTLPLYNYARLLVEKKYNIIAIVELKS KLLFFITLYIAINEFDYQGSAVVMSWAVSYFLRYCLFWWFSKKIELYKNDNLPQNKPAAILKSWWMYIHN QILSQVLNYFTITFDIFIISHFGGLEKVGVYSLCKDATLKISAVLSPVISKLLISHVVNIEENKISPMYK KVYKATIIISISVFGIWALCGSSIIHFIRPQLDQGILTFIIGWSICGILRMMINPIVSIFQATGKTRNEL YVNITSAVSFIFFMGMLSIYFDNIADAVILALICMYSISFVYATVLLRKYFSKIY
Trembl:	A7J0Q3

Literature
First Author: Bartelt, M. "Structure of the O-specific polysaccharide of the O22-antigen (LPS) from Escherichia coli O22:K13". Carbohydrate Research. 254. (1994), pp 203-212 PubMed: [7514095]
First Author: Liu, Y. "Identifying Escherichia coli O22 and O91 serogroups by multiplex PCR of wzx and wzy gene of O-antigen gene cluster". Not specified. (2006),