O-Antigen Description

Serogroup:	O35
Serotype H:	10
Serotype K:
Strain:
Structure:	->3)aLRha(1->2)aLRha(1->3)aLRha(1->2)aLRha(1->3)bDGlcNAc(1-> \| aDGalNAcAN(1->2)
Comment:	- Structure [1]

GTs

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WbpS

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WcnX

GT Name	Donor	Acceptor	Score / e-value
O105-WbsV			126 / 3e-36
O54-O054_07			126 / 3e-36
O120-O120_13			122 / 1e-34

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WcnY

aLRha(1->

GT Name	Donor	Acceptor	Score / e-value
O120-O120_14			145 / 1e-42
O10-O010_09			142 / 4e-41
O146-WbwV			140 / 1e-40
O129-RfbF			140 / 1e-40
O135-RfbF			140 / 8e-41
O13-RfbF			140 / 1e-40
O19-O019_08			139 / 4e-40
O69-O069_11			138 / 8e-40
O19-O019_09			136 / 3e-39
O148-WbbQ			135 / 8e-39
O147-WfbY			135 / 6e-39
O54-O054_10			125 / 3e-35
O150-WfgI			122 / 4e-34
O133-O133_07	aLRha(1->	->2)aLRha ->4)aDGalA	114 / 3e-31
O71-WdaM	aLRha(1->	->4)bDGal	99 / 1e-25
O139-WfaI			93 / 1e-23
O177-WbdY			91 / 4e-23

ncbi CAZy

- Reference [2]

GT name

Donor

Acceptor

Position | Link.Num

Similar to

WcnZ

aLRha(1->

GT Name	Donor	Acceptor	Score / e-value
O119-WekH	aLRha(1->	->3)aDGlcNAc	222 / 6e-73
O48-O048_08			220 / 3e-72
O139-WfaL	aLRha(1->	->3)aDGlcNAc	181 / 6e-57
O75-wekZ			180 / 1e-56
O16-WbbL	aLRha(1->	->3)aDGlcNAc	171 / 4e-53
O39-O039_13			156 / 2e-47
O132-O132_08			135 / 2e-39

ncbi CAZy

- Reference [2]

NMR
1H-NMR		4.69 3.71 3.59 3.32 3.41 3.66 3.90 2.06 4.95 4.35 3.91 3.54 3.71 1.27 5.15 4.05 3.94 3.44 3.81 1.29 4.86 4.11 3.82 3.54 3.76 1.27 5.01 3.78 3.87 3.45 3.99 1.24 5.12 4.16 4.00 4.35 4.82 2.03
Solvent:	D2O
Temp:	50C
pD:	3.2
Ref:	Not Stated NULL ppm
Comment:	1H-NMR [1], pD=3.2. Amide protons at 8.08, 7.80, 7.27 and 7.58
13C-NMR		103.40 56.70 82.30 70.00 76.40 62.00 23.40 175.10 99.50 75.30 79.20 72.20 70.30 17.50 101.70 78.40 70.60 72.90 69.70 17.50 103.10 70.60 78.40 72.10 69.70 17.50 100.60 80.60 70.60 73.10 69.70 17.50 95.60 50.00 67.80 69.50 72.00 174.60 22.80 175.40
Solvent:	D2O
Temp:	50C
pD:
Ref:	Not Stated NULL ppm
Comment:	13C-NMR [1]

Enzymes
Flippase		MYLKILIDNISLVIQYFFGGIAVFYVTPLIVKSVGIHTYGNLAIMFAIVTYISVVIQYSF NLIGPKLIAEGNCKKTFNTIISAKIVLFLLSLIFFLLYFYFFQNKHDDVFLLFLVFPLSW VFNSAWYLQSKGYFVISSVCSIIGSLITFVIAYFFINTTTGLFVPILCLVLSSFINGLLT FFFAVKNNGHIEIVNPLPMLKEGMPLFVSQIISSLYTMSGVFIITYFYGVASAGTYAIVE RFMNLLISLGVLTHVAAYPKLARLFNKDRLEYRKTLLFVIALYTLFSCCVAIVVFNFHQN IVTYMFGSESVNAKELIYSACIFIFVSIYGPVVTGYFTLKVKGRMIITINIIIALLSLLL GVGMLKIVGSSGWLIGLSLAQLLYIAIFFKIFIWGRKECAV
Trembl:	D6BV45
Polymerase		MNGVAKPIIISWFVLCTLVVFFLCSFEQFPDYYSYLNWYELSVSATLNNNWVFFKDPGFY LLSVISNNFDFGIIGAIFFLFIISLSCKIFFCIKLLDWEVLFWVLLLYLSRLFIIHDLVQ YRAGASIGLSALFVYFYLEKKRIKSFFFLSLALSIHLSSLLMVSVIPIAWFLNRNVGGNI IRQIAILLLILSLSLFFDPYVNLLRLVSQFPLMHERIAPYLDGSYLVSNTSMFNSFVIIK IISYVIFFVWICKNKNVGLTNENYLIYLCFFISIVGLFLFWCFRSNDSLSIRFSDFFALY DIVFFALLLNVFDVFGKFIYRYCLLVVVIVFFISSMKLIN
Trembl:	D6BV49

Literature
First Author: Rundlöf, T. "Structural determination of the O-antigenic polysaccharide from Escherichia coli O35 and cross-reactivity to Salmonella arizonae O62". European Journal of Biochemistry. 258(1). (1998), pp 139-143 PubMed: [9851702]
First Author: Liu B. "Development of a serogroup-specific DNA microarray for identification of Escherichia coli strains associated with bovine septicemia and diarrhea". Veterinary Microbiology. 142. (2010), pp 373-378 PubMed: [19932572]